Tissue-Tek VIP 6 AI Operating Manual Download Page 4

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The brand names of products that have been registered or trademarked by

and are owned by Sakura Finetek USA, Inc., Sakura Finetek Japan Co., Ltd.,

and Sakura Finetek Europe B.V. appear here:

Tissue-Tek VIP

Sakura
Tissue-Tek

Tissue-Tek AutoTEC

Uni-Cassette

Tissue-Tek Paraform

Other trademarks referenced in this operating manual:
Tissue Fix

is a registered trademark of Gene Linx International, Inc

Omnifix

is a registered trademark of An-Con Genetics, Inc..

Pen-Fix

is a registered trademark of Richard-Allan Scientific Company

Pro-Par

is a registered trademark of Anatech, Ltd. Corporation

Histosol

is a registered trademark of National Diagnostics

Clear-Rite 3

is a registered trademark of Richard-Allan Scientific Company

Hemo-De

is a registered trademark of Scientific Safety Solvents

Para-Plast

is a registered trademark of Leica Biosystems Richmond, Inc.

Tissue-Prep

is a registered trademark of Thermo Fisher Scientific Inc.

Ameraffin

is a registered trademark of Allegiance Corporation

Printed in U.S.A.

Revised 12 May 2016

0007852-01 Rev. A

Manufactured for:

Sakura Finetek USA, Inc., Torrance, CA 90501 U.S.A.

Sakura Finetek Japan Co., Ltd., Tokyo, 103-0023, Japan

Sakura Finetek Europe B.V., 2408 AV Alphen aan den Rijn, The Netherlands

Summary of Contents for VIP 6 AI

Page 1: ...Operating Manual Tissue TekVIP 6 AI Vacuum Infiltration Processor...

Page 2: ...O p e r a t i n g M a n u a l Vacuum Infiltration Processor Tissue Tek VIP 6 AI...

Page 3: ...1...

Page 4: ...egistered trademark of Anatech Ltd Corporation Histosol is a registered trademark of National Diagnostics Clear Rite 3 is a registered trademark of Richard Allan Scientific Company Hemo De is a regist...

Page 5: ...ing 13 2 4 Positioning the Instrument 14 2 5 Precautions 18 3 CUSTOMIZATION OF SETTINGS 3 1 General Information 19 3 2 Accessing the Menu 19 3 3 Configuring System Utilities 20 3 4 Customizing System...

Page 6: ...1 7 2 Monitored Errors 81 7 3 Retort Fill Error 81 8 SERVICE AND REPLACEMENT PARTS 8 1 Service Information 91 8 2 Replacement Accessory Items 91 8 3 Replacement Parts 91 8 4 Tissue Tek VIP AI Preservi...

Page 7: ...and operate the instrument and monitor the instrument during processing 2 Retort An enclosed stationary chamber that holds the tissue specimens and in which all processing occurs 3 Fume Filter Access...

Page 8: ...vices installed in this instrument by the manufacturer only establish the basis for accident prevention Primary responsibility for accident free operation lies above all with the institution that owns...

Page 9: ...procedures such as continuous mixing The instrument will stop processing and an alarm will sound whenever there is an error condition The instrument can also be connected to an external alarm This al...

Page 10: ...roper solution levels during pump in Figure 1 D 1 6 4 Paraffin Oven The paraffin oven Figure 1 E maintains a set temperature to hold molten paraffin at the proper temperature for use in the infiltrati...

Page 11: ...tch The power switch Figure 1 H is located on the right side of the control panel below the USB port The power switch also acts as an emergency stop switch 1 7 2 USB Port The USB port Figure 1 H is lo...

Page 12: ...er outage signal low battery signal External Signal Output External Alarm Output LAN Terminal Figure 1 I 1 7 4 Power Cord The power cord input Figure 1 J is located on the left rear of the control pan...

Page 13: ...rvoir 1 Paraffin Waste Reservoir 1 Bulk Reagent Reservoirs 2 Capacity up to 300 Uni Cassettes P V Cycle Pressure ambient and vacuum cycles are alternated in the retort during processing 30 180 Seconds...

Page 14: ...lear Rite 3 Hemo De Tissue Tek VIP Paraffin Para Plast Tissue Prep Ameraffin Partial off line fixation rinse Zenkers Bouin s B 5 Incompatible reagents Bleach Per fix Helly s Acetone Tissue Dry 5 or gr...

Page 15: ...ent operating humidity range is 30 85 relative humidity 2 3 Unpacking Unpack the instrument in accordance with the instructions provided with the instrument Unpacking Check List Confirm that all acces...

Page 16: ...2 Pull out the fume filter drawer 3 Remove the plastic wrapping from both carbon filters 4 Place the carbon cartridges side by side in a vertical position in the appropriate location Figure 2 A Figur...

Page 17: ...the bottles manually Be sure the bottles are placed on a level surface Remove the bottle cap by turning it counterclockwise Fill the bottle with the appropriate reagent to the desired solution level R...

Page 18: ...exit the screen If desired the drain fill port can be rinsed using the Rinse function 14 Exchange Confirmation Touch Yes if connection is secure Touch No if connection is not secure and then connect t...

Page 19: ...ir Figure 2 J Figure 2 J 6 Fold the edge of the liner along the edge of the waste paraffin reservoir Figure 2 K Figure 2 K 7 Fold both the front and back edges of the stiffened portion of the liner Fi...

Page 20: ...N PARAFFIN IS VERY HOT AND CAN CAUSE SEVERE BURNS NOTE When the instrument is first powered on it requires 24 hours to completely melt solid paraffin 2 5 Precautions There are several precautions that...

Page 21: ...enu NOTE Login as an Administrator in order to modify system settings and operating parameters To access the Menu 1 From the main system screen touch the Log On button The Enter Password dialog box di...

Page 22: ...to set alarms and signals Manual Operations allows the user to manually do the following pump in pump out and move rotary valve Error Log allows the user to view the error log Part Status allows user...

Page 23: ...of cassettes to be run Available choices are 300 cassettes or 150 cassettes The factory default setting is 300 cassettes Solution Level Safeguard sets the solution level safeguard in use or not in us...

Page 24: ...ng changes Setting User Names and Passwords The system allows for four Administrators and twenty Users From the System Setup screen touch the Password Input button The Password Setup screen displays F...

Page 25: ...s permissions will be changed then touch the Screen Access button The Password Setup Screen Access screen displays Figure 3 K This screen displays three tabbed pages Process Utility and Edit Each tabb...

Page 26: ...be enabled or disabled To use fume filter management Toggle the button at the top left of the screen until it reads In Use To disable fume filter management Toggle the button until it reads Not in Us...

Page 27: ...haracters long Touch Save to save changes and return to the system setup screen or Touch Cancel to return without saving changes Alarm Sound Selection 1 From the System Setup Screen touch the External...

Page 28: ...retort lid heating as automatic heating always on heating or no heat The factory default setting is Always ON Heating Oven Temperature sets the oven temperature for stations 11 14 NOTE The oven temper...

Page 29: ...reen displays Figure 3 W Figure 3 W The Processing Mode Selection screen allows users to adjust the start confirmation start notice bottle check experiment number cleaning cycle defaults cassette coun...

Page 30: ...tem to require a user to log on at the end of a processing run Touch Modify to toggle between On or Off Cleaning Cycle Default after solution exchange Selects a default Cleaning Program Touch Modify t...

Page 31: ...Exit button to return to the Export Data screen To export or view cleaning reports 1 From the Export Data screen touch the Cleaning Report button A Report List displays Select the report to be viewed...

Page 32: ...to return to the Export Data screen To export a system setup data From the Export Data screen touch the System Setup button An export confirmation dialog box displays touch the Exit button to return t...

Page 33: ...rnal Alarm Trigger List configured in the External Alarm setup screen If an event in the External Alarm Trigger Lists is triggered or if the power is turned off the relay will switch to T5 immediately...

Page 34: ...e station number displayed pumping the reagent out of the retort in the station displayed or moving the rotary valve to the next station The System Status Area is located on the right side of the scre...

Page 35: ...ss the Edit Menu 1 Log on 2 From the Main Menu touch the Menu button and then touch the Edit Menu tab to display the Edit Menu Figure 3 II Figure 3 II The following options are available from the Edit...

Page 36: ...LL Figure 3 LL 5 Use the touch keyboard to enter an abbreviated name for the new solution up to eight characters long This name is used for on screen display 6 Touch Edit Abbreviated Solution to save...

Page 37: ...uch keyboard to modify the solution name using up to twenty two characters 4 Touch Edit Solution to save the solution name and return to the Edit Menu or Touch Cancel to return without saving the new...

Page 38: ...gure 3 QQ Figure 3 QQ 3 Use the touch keyboard to modify the solution name using up to twenty two characters 4 Touch Edit Solution Group to save the solution name and return to the Solution Configurat...

Page 39: ...T Figure 3 TT 3 Set the following parameters for the solution configuration Solution Name Select a solution name from the solution name list Touch the Edit Name button to edit the solution name Touch...

Page 40: ...ervoir Bulk Tank 19 Bulk Tank 20 None NOTE When the bulk solution reservoir is in use the solution level for that reservoir is displayed in the same color selected for the solution group Station Color...

Page 41: ...program name using up to twenty two characters Touch Edit Program to save the program name and return to the Edit Menu or Touch Cancel to return without saving the new name 4 Create program steps see...

Page 42: ...correct step Figure 3 AAA 2 Touch the Modify button The Input Time dialog box displays Figure 3 BBB Figure 3 BBB 3 Use the touch keypad to select hours and minutes and adjust accordingly Touch Enter...

Page 43: ...an be selected as Standard 70kPa Middle 37kPa and Pump in 27kPa Processing with a solvent of low boiling point methanol ethanol etc for long hours at high concentration using high P V may cause the so...

Page 44: ...the bottom of the retort to a level sensor providing continuous mixing for defatting at 12 minute intervals NOTE To set Cont1 Cont2 or Cont3 set Solution Manager to SM1 or SM2 If Solution Manager has...

Page 45: ...gram end time is scheduled to end today Touch the Tomorrow button if the program end time is scheduled to end tomorrow Touch the Day After Tomorrow button if the program end time is scheduled for the...

Page 46: ...From the Program List select to highlight the program to be copied and then touch the Copy button The Copy Program screen displays 3 Select to highlight the program to copy the information to or touc...

Page 47: ...le or is unable to pump in during the pump in phase during processing the solution from the previous station of the same group is substituted If there is not a previous station the solution in the nex...

Page 48: ...leted or touch the All Data button Touch Delete to delete all programs A Confirmation dialog box displays 3 Touch Yes to confirm deletion or No to cancel the delete procedure To delete cleaning report...

Page 49: ...bing of the instrument by rinsing with Xylene and alcohol Warm Water Flush Removes precipitates created by the combination of alcohol and NBF by flushing the tubing rotary and gate valves with warm wa...

Page 50: ...UUU Touch Enter to save the new setting and return to the Clean Retort screen or Touch Cancel to return without saving new settings 9 Touch the Number of Cycles field under alcohol and touch Modify T...

Page 51: ...be the first used reagent of the particular group The reagent bottle which held the discarded reagent should be cleaned and or rinsed with clean reagent Refill the reagent bottle with fresh reagent a...

Page 52: ...ange External Drain External Fill Fill from Bulk Reservoir and Drain Fill from Bulk Reservoir NOTE Fill from Bulk Reservoir and Drain Fill from Bulk Reservoir are not available for the solution groups...

Page 53: ...he station in progress is displayed Station color indicates the station color selected for that station Station number indicates the station number of that station Solution name indicates the solution...

Page 54: ...tion reservoir the automatic transfer is completed after emptying the solution bottle at the last station of that solution group In this case the last station needs to be filled with a new solution ma...

Page 55: ...containing a defatting solution are not subject to automatic transfer NOTE A mixed solution is not automatically prepared and supplied for automatic transfer normally performed for solution groups onl...

Page 56: ...the station Figure 3 EEEE 1 Touch Menu to display the Process Menu Screen 1 Touch Detailed Monitor to view the Defatting Solution Preparation Detailed Monitor Screen Figure 3 FFFF Figure 3 FFFF Syste...

Page 57: ...tations To select all stations touch All Stations 3 Once the station s have been selected touch Clear to reset the solution usage A confirmation dialog box appears Touch Yes to reset the solution usag...

Page 58: ...lutions to be exchanged for that day is displayed on the right The Calendar can display the past 3 months of exchange history and the upcoming 12 months of scheduled exchanges Touch the Export Data bu...

Page 59: ...an only be added to station 14 The melting process requires 9 hours to complete Molten Paraffin Notification Procedure The paraffin melt process can be stopped by a user if the paraffin becomes molten...

Page 60: ...displays notifying users of the predicted end time Touch Cancel to return to the Process Menu NOTE When the VIP 6 AI is in a melting state a paraffin icon displays on the paraffin station that is in...

Page 61: ...Administrator in order to modify system settings and operating parameters To Log On 1 From the main Process Monitor screen touch the Log On button The Enter Password dialog box displays 2 Use the tou...

Page 62: ...load for a processing run Touch Modify to choose either 150 Cassettes or 300 Cassettes If the number of cassettes is changed from 150 to 300 a confirmation window appears to request whether or not to...

Page 63: ...h Clear to erase the settings Touch Cancel to exit without saving To adjust the experiment number The Experiment Number field is used to display a reference number associated with the tissue samples N...

Page 64: ...er is activated the solution group designated for automatic transfer is discontinued Solution and paraffin rotations occur differently when using Automatic transfer Solution rotation automatic Transfe...

Page 65: ...Retort Lid Unlocked has disappeared from the display 4 Tissue Tek Paraform Sectionable Cassettes can be processed in the VIP using Tissue Tek AutoTEC magazines product code 7005 The retort can accommo...

Page 66: ...nu button to view the Process Menu screen Touch Log Off to log off of the system Touch the Detailed Monitor button to view the Tissue Processing Delayed Start Detailed screen Figure 4 J Figure 4 J The...

Page 67: ...dify button to select either Add Cassettes Update Cassettes or Remove Cassettes 2 Use the keypad to enter the amount of cassettes being added updated or removed 3 Touch the Enter button to save 4 Touc...

Page 68: ...the Clean Retort Tab information pertaining to the clean cycle is displayed Touch the Edit Clean Programs button to edit the current cleaning program Touch the Modify button to change the Dechlorofor...

Page 69: ...Detailed Monitor to switch the display to the Clean Retort Detailed Monitor Screen Figure 4 P Figure 4 P The Clean Retort Detailed Monitor displays the following information System status area The cur...

Page 70: ...tion chosen to perform the warm water flush The lamp icon will illuminate 2 Touch the Drying Cycle button to enable or disable the drying cycle If the drying cycle is enabled the lamp icon is illumina...

Page 71: ...cleaning status remaining warm up time and expected end time for a warm water flush 1 Touch Menu to display the Process Menu Screen 2 Touch Detailed Monitor to switch the display to the Warm Water Fl...

Page 72: ...ger icon displays 2 Touch Drain Fill Port Rinse if using alcohol from an external container to rinse the retort and lines after a solution exchange 3 Touch Drying Cycle to perform a drying process fol...

Page 73: ...is area Cleaning status area The current status of the cleaning in progress is displayed Station color indicates the station color selected for that station Station number indicates the station number...

Page 74: ...72...

Page 75: ...Cassette Baskets with Dividers and Handles 7005 AutoTEC 32 Cassette Magazines Product Code Description 7093 AutoTEC a120 20 Cassette Magazines Product Code Description F60 500 00 Reagent Bottle A MO7...

Page 76: ...ries If located within the United States contact the Order Management Department of Sakura Finetek USA Inc by calling toll free 1 800 725 8723 Alternately or in countries other than the United States...

Page 77: ...To disinfect the touch panel screen spray 70 80 alcohol onto a soft cloth and gently wipe the screen Do not spray any liquid directly onto the screen The acrylic doors may be cleaned with a glass clea...

Page 78: ...ean cycle These reagents should never become sludgy or extremely viscous that would pose an internal clogging risk If a xylene substitute is used or additional items are added to the Clean Cycle proce...

Page 79: ...in the photo below NOTE The level sensors are located on the front wall of the retort Figure 6 E Insert the brush into the sensor hole Figure 6 F 4 Turn the brush 90 degrees to the right or left to p...

Page 80: ...ce of the groove Move the brush to the right and left in small motions about 10 times NOTE If you turn the brush vigorously it may slide out of the sensor hole In that case insert the brush tip into t...

Page 81: ...Replacement WARNING AN ACTIVATED CARBON FILTER MUST BE INSTALLED IN THE INSTRUMENT AS ALL TIMES TO ENSURE PROPER FILTERING OF FUMES GENERATED DURING PROCESSING The fume control system serves to reduc...

Page 82: ...a connection problem will be a failure of the bottle connection check Replace bottles that are cracked or deteriorated see Section 8 Service and Replacement Parts Disinfection The instrument can be di...

Page 83: ...m press the EXIT button on the warning message or the Alarm Off button The Alarm Off stops the alarm from sounding but leaves the error message window open Each error that results in the display of an...

Page 84: ...low in the message box When only the troubled station shown in red is displayed the processing run is interrupted with the solution of the troubled station remaining in the retort Follow the instructi...

Page 85: ...screen Figure 7 C 2 The password request window is displayed Enter an appropriate password to abort the processing run Figure 7 D 3 Once the password is accepted the processing run is aborted and the...

Page 86: ...ayed Touch the Menu button Figure 7 G 6 The Process Menu screen is displayed Touch the Utility Menu tab Figure 7 H 7 Touch the Manual Operations icon in the Utility Menu Figure 7 I 8 Touch the Drain R...

Page 87: ...s filled with the appropriate amount of solution normally filled up to the specified solution level that assures the solution level during the processing Add the solution as necessary NOTE When the re...

Page 88: ...the solution bottle Properly close the retort lid Connect the hose to the fill drain port Contact Sakura Technical Support Low solution was detected during pump in The solution bottle or paraffin con...

Page 89: ...nutes The solution bottle is detached Reconnect the solution bottle Line is clogged Contact Sakura Technical Support Hose was not connected to the drain fill port when an attempt was made to supply fr...

Page 90: ...Pump in failure during or after second pump in cycle Check the cause and action for Error 11 17 Solution did not reach the level sensor at the assurance position after pump in Solution did not reach t...

Page 91: ...her substance heated to 80 C or above in station 11 132 Paraffin station 12 exceeded 80 C Paraffin or other substance heated to 80 C or above was placed in station 12 Wait for the station 12 temperatu...

Page 92: ...to the drain fill port used for supplying to the right bulk reservoir station 20 Highly viscous solution was used If any solution not specified in Usable Solutions is to be used consult the dealer Ot...

Page 93: ...ura Finetek USA Inc by calling toll free 800 725 8723 Option 2 In countries other than the United States contact the nearest authorized Sakura instrument distributor or representative for service info...

Page 94: ...No If so have they all been corrected and cleared Yes No If unable to clear a warning what does it say ________ Are any errors listed in the Error Information screen If so please list the information...

Page 95: ...run and the other for a rush or biopsy run Example reagents and concentrates are shown also The programs are intended to be used as a guide only modify them to satisfy the individual needs of your la...

Page 96: ...V Slow 13 VIP Paraffin 0 30 58 P V Slow 14 VIP Paraffin 0 30 58 P V Slow Total Processing Time 12 7 Hours ROUTINE OVERNIGHT RUN Station No Solution Conc Time hr min Set Temp P V Mix 1 Neutral Buffered...

Page 97: ...LY 95 PROGRAM Station No Solution Conc Time hr min Set Temp P V Mix 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Total Processing Time PROGRAM Station No Solution Conc Time hr min Set Temp P V Mix 1 2 3 4 5 6 7 8...

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