5
ChromQuest Diagnostics for the PDA Detector
Verifying Operational Performance
72
Surveyor PDA Plus Detector Hardware Manual
Thermo Scientific
Verifying Operational Performance
If you move your PDA detector, replace lamps, or install a new flow cell, the system
performance might change. Verify that the detector is running as desired by performing the
following procedures:
•
Verifying the Performance of the Detector
•
Recording the Performance of the Lamps
Verifying the Performance of the Detector
To verify the proper operation of the detector from ChromQuest
1. Open the online Instrument window for your instrument.
2. Create and download a method that pumps 100% HPLC-grade methanol or
HPLC-grade water at a constant flow rate of 1 mL/min.
3. Check the status of the lamps:
a. On the menu bar, choose
Control > Instrument Status
to display the Instrument
Status window.
b. Click the
Surveyor PDA Plus
tab to display the Surveyor PDA Plus Instrument
Status page.
c.
Click
Diagnostics
to open the PDA Plus Diagnostics dialog box.
d. Click the
Lamps
tab to display the lamps page. Note the status and usage for each
lamp.
e.
If they are not already on, turn on the deuterium (D
2
) and tungsten (W) lamps. Wait
for 1 hour for both lamps to equilibrate.
4. Perform a dark current calibration, and then perform a wavelength calibration. Use the
wavelength calibration file appropriate for your normal operation. Do not forget to apply
the results by clicking
Apply.
5. View an intensity scan of the deuterium (D
2
) lamp:
a. Click the
Lamps
tab to display the Lamps page.
b. If the tungsten lamp is not off, turn it off.
c.
Click the
Control
tab to display the Control page.
d. In the Mode
area, select the
Intensity
option to set the units to intensity.
e.
Click
Default
.
f.
In the Discrete Channels area, set Channels A, B, and C to monitor diodes 35, 52,
and 77 respectively (see
