4
Xcalibur Diagnostics for the PDA Detector
Adjusting the Attenuators
Thermo Scientific
Surveyor PDA Plus Detector Hardware Manual
59
5. Make sure that the lamps are on as follows:
a. Choose
Surveyor PDA Plus > Direct Control
.
The Surveyor PDA Plus Direct
Control dialog box appears.
b. Click the
Configuration
tab to display the Configuration page.
c.
Verify that both lamps are on. If they are not on, click
Turn On
for both lamps.
6. Load the display method to the detector as follows:
a. In the Surveyor PDA Plus Direct Control dialog box, click the
Display
tab to open
the Display page.
b. In the Control area, click
Load Method
and choose your attenuator method file
from the list.
c.
Click
OK
.
7. Start the data stream and adjust the attenuators as follows:
a. In the Control area, click
Start
. The spectrum of light intensities appears in the top
window (see
).
For the UV region, the diode of maximum intensity is between diode 30 and
diode 50. For the Visible region, the diode of maximum intensity is between
diode 400 and diode 500. Ignore the spike at approximately diode number 380. This
spike is an emission line of the deuterium lamp.
b. Adjust the attenuator with the left tab on the PDA (UV attenuation) to achieve a
maximum value from 750000 to 775000 intensity counts in the region between
diode number 30 and diode number 50.
c.
Adjust the attenuator with the right tab (Visible attenuation) to achieve a maximum
value from 750000 to 775000 intensity counts in the region between diode
number 400 and diode number 500.
8. After you finish adjusting the attenuators, replace the flow cell access cover and close the
front doors of the detector.
