4
Xcalibur Diagnostics for the PDA Detector
Calibrating the PDA Detector
Thermo Scientific
Surveyor PDA Plus Detector Hardware Manual
33
b. In the
Wavelength Calibration
area, click
Calibrate
to start the Wavelength
Calibration wizard.
The preconditions page appears (see
).
Figure 26.
Preconditions page of the Wavelength Calibration wizard
c.
Read the preconditions and verify they have been met:
• If the preconditions have been met, click
Next
to proceed with the calibration.
• If the preconditions have not been met, click
Cancel
to exit the wizard and
prepare the PDA detector for calibration.
4. On page two of the Wavelength Calibration wizard (see
), do the following:
a. Choose a peak set from the list.
The peak set should span the wavelengths you use under normal operation
conditions. In
, the Holmium Oxide 5 peak set is selected. Using this
wavelength list, Xcalibur calibrates the detector at each of the five wavelengths
shown.
Note
You can press the Cancel buttons on the pages of the Wavelength
Calibration Wizard at any time a calibration is in process to stop it.
Note
Xcalibur has four calibration files to choose from. For example, the
HolmiumOxide UV file contains five wavelengths in the UV region while the
other files use sets of wavelengths from both the UV and Visible wavelength
regions. The holmium oxide absorbance maxima are selected from a spectrum
published in “Holmium Oxide Solution Wavelength Standard from 240 to
640 nm - SRM 2034 (NIST Special Publication 260-54)”
.
