Thermo Scientific ISQ, User Manual

Page: 66 / 152

4
Creating a Method
Creating a Method for the GC
44 ISQ User Guide Thermo Scientific
Figure 32. Setting the GC Oven Parameters on the TRACE 1300 or TRACE 1310
a. You can select a maximum of 32 temperature ramps, each with their own ramp rates,
final temperatures and hold times. A typical program will have one or two ramps.
The GC temperature profile is the primary method for separating your analytes from
each other, the solvent, and the matrix. Your temperature profile will have to be
optimized for your analysis needs.
b. You can also select the maximum allowed oven temperature, which should be set to
the maximum temperature allowed by your method, not the maximum allowed by
your column. The maximum temperature allowed by the GC is 450 °C. This will
prevent you from accidentally using a temperature that will damage your column.
The prep-run timeout is the maximum amount of time that the GC will wait before
it gives up on an injection. As an example, with the default value of 10 minutes, if the
GC is ready to receive an injection, but does not receive it after ten minutes, the GC
will stop waiting. This usually occurs in case of an error. The equilibration time is a
delay between when the GC is at temperature and when the GC reports as being
ready. This delay is typically set to 0.5 minutes.
2. The TRACE 1300 and 1310 also have the option to enable the use of cryogenics to cool
the oven. If this option is selected, then the minimum allowed temperature in a
temperature ramp will fall from 0 °C to -99 °C. The GC also allows the use of a post run
column cleaning. This is not typically used because the material that is purged from the
column in this step would go into the ISQ, which can lead to contamination. If you want
CAUTION - INSTRUMENT DAMAGE. Be sure not to overheat the GC column or it may
contaminate the ISQ.