Thermo Scientific ISQ, User Manual

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4
Creating a Method
Creating a Method for the ISQ Mass Spectrometer
Thermo Scientific ISQ User Guide 37
To use the hot needle technique, select the standard injection depth and use a
pre-injection dwell time of at least 2 seconds. To use the cold needle technique,
select a minimum injection depth and do not use a pre-injection dwell time. The
post injection dwell time can be used to bake out the needle and ensure that all
semi-volatile compounds are cleaned out of the system before the next injection
is made.
Creating a Method for the ISQ Mass Spectrometer
Click the ISQ icon in the left pane to create a method for the ISQ mass spectrometer.
1. To create a acquisition method from the Method Type pull-down menu, select
Acquisition-General . An Acquisition method is used to collect data.
Figure 29. Creating a General Acquisition Method for the ISQ Mass Spectrometer
2. Set the MS Transfer Line Temperature . This field represents the temperature of the
transfer line, which is the tube that contains the column as it leaves the GC oven and
enters the ISQ mass spectrometer. The maximum allowable temperature is 400 °C.
However, you will damage the column and contaminate the ISQ instrument if you set the
transfer line temperature above the maximum allowed temperature of the column.
Note
The maintenance method is used to bake out or cool down the ISQ instrument
during a sequence. This can be useful if you know you want to perform these tasks and
you want to do them in an automated way as part of your data acquisition.