Thermo Scientific ISQ, User Manual

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8
Troubleshooting
Investigating Baseline Issues
108 ISQ User Guide Thermo Scientific
Investigating Baseline Issues
Table 2. Troubleshooting Baseline Issues in Your Data (Sheet 1 of 2)
Behavior Characteristic Cause Remedy
Drifting Baseline General Stationary phase has
accumulated in column Replace the column or cut off the end of
the column.
Chromatographic baseline
is high Replace the column or cut off the end of
the column.
Carrier gas pressure is too
low Check for leaks in injector or flow path.
Replace the carrier gas cylinder if it is
empty or low. Increase the pressure if
maximum injector pressure in method is
greater than carrier line pressure set by
regulator. Make sure the vacuum
compensation is on.
Carrier gas flow is drifting Check for leaks in injector or flow path.
Check the carrier gas tank.
Impurities have
accumulated in column Run solvent blank to remove impieties.
If impurities persistent after multiple
solvent blanks: Inject solvent from a
different source. Change syringe, liner
and septum. Clean injector. Check
impurity levels in your gas. Use the
correct gas purity and filter.
Falling Carrier gas leak in the
system Perform a leak test and tighten the
connections to the carrier gas line if leak
is found.
Column is baking out Wait for the column to stabilize.
Rising Impurities have
accumulated in column Check impurity levels in the gas source.
Use correct gas purity.
Abnormal rise in
baseline when oven
temperature is high Impurities have
accumulated in column Recondition or replace the column.