2
Changing the Column
Connecting the Column to the Transfer Line
20
ISQ User Guide
Thermo Scientific
16. Loosen the transfer line nut from the column measuring tool.
17. Remove the column, transfer line nut, and ferrule from the column measuring tool,
making sure not to move the septum from its location on the column.
18. Insert the column into the transfer line.
Figure 17.
Inserting the Column into the Transfer Line
19. Tighten the spring loaded transfer line nut until it is just secure enough so that you
cannot move it.
20. Loosen the nut by turning it exactly 1 flat backward.
21. Position the column in the transfer line using the cut septum to measure the correct
length you should insert the column.
Note
The ferrule should still be able to move on the column. Use the septum to mark the
correct location where the column should exit the nut.
Transfer Line
Column
Spring Loaded Transfer
Line Nut
Septum
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