1
2
3
1
Figure 5 Tube types and thickness
Cell isolation kit (left) and Bead removal kit (right)
1
PVC: 0.094 in ID 0.157 in OD; 2.4 mm ID 4.0 mm OD
2
Silicone: 0.125 in ID 0.25 in OD; 3.2 mm ID 6.4 mm OD
3
Bioprene: 0.125 in ID 0.25 in OD; 3.2 mm ID 6.4 mm OD
The dead volume is calculated based on tube diameter and length, using the following formula:
V = A h
Since the area of a circle = π r
2
, then the formula for the volume of a cylinder is:
V = π r
2
h
Note:
The volume in mL for each tube is indicated in Figure 6. This enables you to adjust for
modifications to the input/output line, by e.g. welding particularly short or long tube lines. While
it is recommended to keep the welding distance from the Single-Use Kits to cell/bead/buffer bags
consistent between runs using the same protocol, note that the dead volume per inch/cm is relatively
low. Millimeter precision is therefore not necessary when welding bags onto the Single-Use Kits from
run to run.
Chapter 1
Product information
System overview
1
CTS
™
DynaCellect
™
Magnetic Separation System User Guide
19
Summary of Contents for A55867
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