Sigma 3-30K Operating Manual Download

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Version 12/2008, Rev. 1.11 of 27/01/2012

Translation of the original operating manual

Operating Manual

Refrigerated Centrifuge

3-30K

from serial no. 128496

Please retain for later use!

Summary of Contents for 3-30K

Page 1: ...Version 12 2008 Rev 1 11 of 27 01 2012 Translation of the original operating manual Operating Manual Refrigerated Centrifuge 3 30K from serial no 128496 Please retain for later use...

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Page 3: ...by Sigma Laborzentrifugen GmbH An der Unteren S se 50 37520 Osterode am Harz Germany Phone 49 0 55 22 50 07 0 Fax 49 0 55 22 50 07 12 E mail info sigma zentrifugen de For service please use our servic...

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Page 5: ...or 15 3 4 Operating Personnel 15 3 5 Informal Safety Instructions 15 3 6 Safety Instructions 16 3 6 1 Electrical Safety 16 3 6 2 Mechanical Safety 16 3 6 3 Fire Prevention 17 3 6 4 Chemical and Biolog...

Page 6: ...ly Sealed Lid 25 6 2 2 3 Installation of Accessories 25 6 2 2 4 Adapters 26 6 2 2 5 Tubes 26 6 3 Spincontrol Comfort 27 6 3 1 Operating Panel 27 6 3 2 Centrifuge Display 27 6 3 3 Manual Mode 28 6 3 3...

Page 7: ...ugation Run 56 6 4 4 Program Mode 57 6 4 4 1 Loading a Program 57 6 4 4 2 Saving a Program 58 6 4 4 3 Deleting a Program 58 6 4 4 4 Automatic Program Rotation 59 6 4 4 5 Options for Data Input and Out...

Page 8: ...le Accessories 73 11 1 1 Maximum speed for tubes 78 11 2 Grafical Representation of the Rotors 79 11 3 Acceleration and Deceleration Curves 80 11 3 1 Linear Curves 80 11 3 2 Quadratic Curves 81 11 4 F...

Page 9: ...driven machines that separate liquids from solid matter liquid mixtures or solid mixtures by centrifugal force They are solely intended for this purpose Any other use beyond this area of application...

Page 10: ...ny othe way neither in full nor in parts Non compliance may be prosecuted under criminal law 1 5 Standards and Regulations EC Declaration of Conformity page 89 1 6 Scope of Supply The centrifuge compr...

Page 11: ...ing manual 2 Design of the Centrifuge 2 1 Overview 1 Mains power switch 2 Stop key 3 Lid key 4 Lid 5 Display 6 Start key 7 Function knob Fig 2 1 Total view of the centrifuge 8 Name plate see page 12 9...

Page 12: ...rer and registered office 2 Type name 3 Serial number 4 Max speed 5 Kinetic energy 6 Max density 7 Nominal voltage 8 Input fuse 9 Symbol for special disposal see chapter 9 10 CE mark in accordance wit...

Page 13: ...Ein Netzverbindung On Power Marche mise sous tension O Aus Netzverbindung Off Power Arr t mise hors tension Schutzleiteranschluss Protective earth ground Liaison la terre Erde Earth ground Terre Netzs...

Page 14: ...endangering injuries This symbol stands for a direct hazard to the life and health of persons due to electrical voltage Non observance of these symbols causes serious health problems up to life endan...

Page 15: ...s intended see chapter 1 2 Intended Use page 9 of this Operating Manual take measures for the safe opening of centrifuges 3 4 Operating Personnel Persons operating the unit must be familiar with the f...

Page 16: ...when the rotor is in motion Do not use the centrifuge if it was installed incorrectly Do not use the centrifuge without panels Do not use the centrifuge if the rotors and inserts show signs of corros...

Page 17: ...practices are adhered to accordingly Spin infectious material in sealed rotors and buckets only in order to prevent the material from leaking into the centrifuge Infectious toxic pathogenic and radio...

Page 18: ...entrifuge if the rotor is loaded asymmetrically Do not use the centrifuge with tubes that are excessively long 3 6 6 Resistance of Plastics Chemical influences have a strong effect on the polymeric ch...

Page 19: ...drive is completely separated from the mains power supply i e the centrifuge cannot be started see 7 1 1 Emergency Lid Release 3 7 2 Standstill Monitoring Opening of the centrifuge lid is only possibl...

Page 20: ...he Event of Hazards and Accidents If an emergency arises switch off the centrifuge immediately If in doubt call the emergency doctor 3 9 Remaining Hazards The unit was built state of the art and accor...

Page 21: ...centrifuge only in dry rooms The storage temperature must be above 20 C If you would like to store it for more than one year or if you intend to ship it overseas please contact the manufacturer 4 3 No...

Page 22: ...rox 100 kg Retain the packaging for any possible future transport of the centrifuge 5 2 Transport Safety Device The transport safety device consists of two plastic screws which are located at the bott...

Page 23: ...even surface Attention During transport from cold to warmer places condensational water will collect inside the centrifuge It is important to allow sufficient time for drying min 24 h before the centr...

Page 24: ...ot hold your fingers between the lid and the housing when closing the lid Risk of crushing 6 2 2 Installation of Rotors and Accessories 6 2 2 1 Installation of the Rotor Open the centrifuge lid by pre...

Page 25: ...d lid after loosening the rotor tie down screw Follow the safety instructions and hazard warnings in chapter 3 1 Rotor tie down screw 2 Lid 3 Lid seals Fig 6 1 Angel rotor with a hermetically sealed l...

Page 26: ...Loading the edges of a bucket only is not permissible 6 2 2 5 Tubes Load the tubes outside of the centrifuge Liquids in the buckets or multiple carriers cause corrosion Fill the tubes carefully and a...

Page 27: ...Panel The centrifuge is started directly via the operating panel When the centrifuge is switched on all of the operating keys and displays will be illuminated for a short time It is now ready for oper...

Page 28: ...before starting a new centrifugation run 6 3 3 3 Interrupting a Deceleration Process Press the start key during a deceleration process in order to interrupt it and to restart the centrifuge 6 3 3 4 S...

Page 29: ...ing the speed Relative Centrifugal Force RCF The relative centrifugal force is the acceleration that the sample is subjected to The set value of this parameter is displayed in the upper section of thi...

Page 30: ...r clockwise or Starting with the time setting 09 59 turn the knob clockwise to the next setting HOLD will be displayed After the start of the centrifuge the elapsed time will be displayed Fig 6 10 Dis...

Page 31: ...st Rotor Selection list Automatical Rotor Identification This field shows the rotor that is currently being used Fig 6 13 Calling up the rotor selection list Selection and change of the rotor number o...

Page 32: ...ited and if there are no rotor bucket combinations a message will be displayed This prevents the maximum permissible speed from being exceeded 6 3 3 6 Parameter Menu For information on how to set the...

Page 33: ...or temperature will not fall below 0 C if it is at a standstill Rapid cooling program RAPID TEMP Precooling at a standstill may distort the measurement results and subsequently cause increased wear of...

Page 34: ...ly when the rotor is at a standstill When the lid is open the cooling is not active Samples may warm up 6 3 3 7 Configuration Menu In the configuration mode the default settings can be customised and...

Page 35: ...e standard size Speed enlarged zoomed The RCF display is eliminated RCF enlarged zoomed The speed display is eliminated Select the Display field in the configuration overview and confirm the selection...

Page 36: ...EPROM version number of cycles the total runtime as well as software version and date In the menus Cycles Info and Sensor the values can neither be entered nor changed Reset This function resets the...

Page 37: ...nfirm the selection The curve that is created will be displayed together with the maximum curve speed Fig 6 19 Time value input into interval no 2 Activate the time display of the second interval and...

Page 38: ...to the set speed for the centrifugation run The sum of the intervals corresponds to the set time of the centri fugation run The last speed checkpoint of an interval equals 0 6 3 3 9 Modification of t...

Page 39: ...4 Program Mode A program includes all of the data that are required for a centrifugation run Attention Temperature profiles cannot be created With a program certain sedimentation results can be repea...

Page 40: ...next to the program selection list and confirm the selection An overview of all of the programs that have already been saved will be displayed The current setting will be displayed under Select a pro...

Page 41: ...ng a program Select the double arrow next to the program selection list and confirm the selection An overview with all of the programs that have already been saved will be displayed Select the program...

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Page 43: ...displays will be illuminated for a short time It is now ready for operation 1 Start key 2 Centrifuge display 3 Function knob 4 Stop key 5 Lid key Fig 6 27 Operating Panel Spincontrol Professional 6 4...

Page 44: ...must be opened before a new centrifugation run can be started A fast stop can also be triggered during a normal deceleration e g in order to speed up the deceleration When a fast stop is triggered FA...

Page 45: ...centrifugal force is the acceleration that the sample is subjected to The set value of this parameter is displayed in the upper section of this field with the actual value shown below The values are...

Page 46: ...lashes when it is activated Select the time 0 00 00 and confirm the selection Infinite will be displayed After the start of the centrifuge the elapsed time will be displayed Deactivate the continuous...

Page 47: ...are different rotor bucket combinations for this rotor the system will automatically select the rotor with the lowest speed The system will display a corresponding message so that the rotor can be sel...

Page 48: ...o a standstill Deceleration curves are inverted images of the acceleration curves and are labelled with identical numbers Deceleration curve no 0 represents a brakeless deceleration 6 4 3 6 Profile Me...

Page 49: ...d causes the compressor to freeze overs At temperatures below 0 C aqueous liquids will freeze making sedimentation impossible Ensure that the rotor temperature will not fall below 0 C if it is at a st...

Page 50: ...cooling phase in order to prevent the system from reheating An entry will interrupt the rapid cooling program There will be no message when the set temperature is reached If no new runtime is entered...

Page 51: ...tarted at any time Standstill cooling and precooling are also possible at any time Lock In order to prevent any unauthorised use of the centrifuge the following functions can be blocked Saving of prog...

Page 52: ...nterdependent For the first interval a linear or quadratic rise can be selected under QUAD LIN All of the other intervals are linear The field Total shows the total runtime of the process The maximum...

Page 53: ...n is active the drive will be disconnected if the actual speed is below the set speed As a result the rotor decelerates and stops in a brakeless manner A brakeless deceleration in particular with heav...

Page 54: ...although the option is selected Start delay If the start delay function is active the centrifuge will not start until the preset time has elapsed The symbol will be displayed in the time field Displa...

Page 55: ...ilable if the centrifuge is equipped with the option for the input and output of data external signal floating switch see 6 4 4 5 System The following system data are displayed Cycles Info Sensor In t...

Page 56: ...box for changing the contrast 6 4 3 11 Modification of Parameter Values during the Centrifugation Run see 6 4 3 4 Selection Display and Modification of Data Modification possible Modification impossib...

Page 57: ...ms can be stored under the numbers 1 60 The rapid cooling program RAPID_TEMP does not occupy any storage location and cannot be delted It is used to cool the centrifuge without vessels means that the...

Page 58: ...acters can be entered when the cursor flashes in the text field Turn the function knob in order to select a character and press it to confirm the selection Then press the knob again in order to enter...

Page 59: ...he next program will be loaded and started automatically The rotation ends with the first empty location and restarts from the beginning Example 1 Loading of sample 1 Rotation sample 1 sample 2 sample...

Page 60: ...operated again Type of error Possible reason Correction No power in the mains supply Check fuse in the mains supply Power cord is not plugged in Plug in power cord correctly Fuses have tripped Reactiv...

Page 61: ...1 Possible error causes 7 1 1 Emergency Lid Release In the event of a power failure it is possible to manually open the centrifuge lid Switch off the mains power switch and disconnect the power cord...

Page 62: ...on With error 34 35 36 the centrifuge will stop with error 37 38 error message only 40 45 Temperature error only for refrigerated centrifuges allow to slow down power off on allow to cool down provide...

Page 63: ...t SIGMA Laborzentrifugen GmbH An der Unteren S se 50 37520 Osterode Germany Tel 49 0 55 22 50 07 84 25 Fax 49 0 55 22 50 07 94 25 E mail service sigma zentrifugen de outside Germany contact our agency...

Page 64: ...agents with abrasive particles Do not expose the centrifuge and rotors to intensive UV radiation or thermal stress e g by heat generators 8 1 Maintenance 8 1 1 Centrifuge Disconnect the power cord fr...

Page 65: ...ic radioactive or pathogenic contamination Dry the accessories with a soft cloth or in a drying chamber at approx 50 C 8 1 2 1 Plastic Accessories The chemical resistance of plastic decreases with ris...

Page 66: ...all glass particles e g with a vacuum cleaner Replace the rubber cushions since even thorough cleaning will not remove all glass particles Glass particles will damage the surface coating e g anodizin...

Page 67: ...ce see 7 3 Service Contact 8 2 Sterilization and Disinfection of the Rotor Chamber and Accessories Use commercially available disinfectants such as for example Sagrotan Buraton or Terralin available a...

Page 68: ...s may deform during autoclaving Accessories max temp C min time max time max cycles Glass tubes 134 138 3 40 Polycarbonate tubes 115 118 30 40 20 Polypropylene copolymer tubes 115 121 30 40 30 Teflon...

Page 69: ...to ageing This is why we recommend having the centrifuge checked by the manufacturer during an inspection once per year in the operating state and once every three years in the dismantled state Rubbe...

Page 70: ...If no such declaration is enclosed in the return package we will perform the decontamination at your expense 2 Form for the return of defective parts This form is for the product related data They fac...

Page 71: ...t permissible to dispose of the unit among household trash You can return these centrifuges free of cost to SIGMA Laborzentrifugen GmbH Ensure that the unit is decontaminated Fill in a declaration of...

Page 72: ...0 sec 9 h 59 min 10 sec 99 h 59 min 20 to 40 C 20 to 60 C 50 60 Physical data Height mm Height with opened lid mm Width mm Depth mm Weight kg EMC as per EN 61326 Noise level dBA 460 890 570 616 100 Cl...

Page 73: ...ers no 13012 and 16 PS tubes 15 ml no 15020 and rubber cushions no 16015 max radius 13 4 cm min Radius 5 1 cm 5 000 3 745 13009 Multiple carrier for 5 tubes 5 ml round and flat bottom incl rubber cush...

Page 74: ...set 2 pcs max radius 14 3 cm min radius 3 3 cm 5 000 3 996 11136 Drum rotor for 6 cassettes no 14000 14002 14020 14021 for reaction vials max 60 192 pcs incl lid no 17826 max radius 7 0 cm min radius...

Page 75: ...x 9 3 cm radius min 3 1 cm angle 25 14 000 20 379 12150 Angle rotor 6 x 50 ml for tubes e g no 13055 15051 15052 incl hermetic aluminium lid no 17837 max 28 8 mm radius max 8 4 cm radius min 2 1 cm an...

Page 76: ...6 1 x 81 1 mm incl screw cap suitable for 12111 12157 15010 Polycarbonate tube 10 ml 16 1 x 81 1 mm incl screw cap suitable for 12168 13084 17355 17655 18010 15039 Polypropylene Co Polymer PPCO tube 1...

Page 77: ...4 000 x g 16 x 100 mm graduated 0 1 ml up to 10 ml in 0 1 ml increments suitable for 13012 14030 17215 15085 Centrifuge glass tube 15 ml incl screw cap with PTFE faced rubber liner 17 5 23 5 x 106 mm...

Page 78: ...1 Rubber adapter for special glass tube 30 ml no 15033 15086 for glass tube 25 ml no 15025 suitable for 12155 12159 1 set 2 pcs Further accessories available upon request 11 1 1 Maximum speed for tube...

Page 79: ...epresentation of the Rotors The graphical representation of the rotors shows the maximum and minimum radii of the accessories used If necessary the values must be manually calculated see 11 4 1 RCF pa...

Page 80: ...ration curves and are assigned the same numbers An exception is curve 0 It decelerates brakeless In general the runtime until the set speed is reached depends on the moment of inertia of the rotor Lin...

Page 81: ...are inverted images of the acceleration curves and are assigned the same numbers Quadratic slope curves 10 19 Curve 19 is a special case compared to the other curves The centrifuge accelerates with ma...

Page 82: ...pecified independently They are interrelated via the following formula RCF 11 18 x 10 6 x r x n2 If two values are given the third value is determined by the equation If then the speed or the rotation...

Page 83: ...3 30K Appendix Version 12 2008 Rev 1 11 of 27 01 2012 83 Translation of the original operating manual Fig 11 7 Speed Grravitational Field Diagram...

Page 84: ...15P 1 15PK 12124 5 years 20x 1 15 1 15K 1 15P 1 15PK 12126 5 years 20x 1 15 1 15K 1 15P 1 15PK 9100 15 000 4 15C 4K15C 4 16 4 16K 6 15 6K15 6 16 6 16K without engraving only spincontrol professional...

Page 85: ...100 3 4 4 1 4 4 2 1 1 Antimony trichloride SbCl3 90 1 4 1 4 1 1 1 4 Benzaldehyde C7H6O 100 1 3 4 1 1 3 4 4 1 1 Benzene C6H6 100 3 2 4 1 3 4 4 1 1 Boric acid H3BO3 aqueous 1 3 1 2 1 1 1 Butyl acrylate...

Page 86: ...ogen chloride HCl 5 1 4 1 4 1 1 1 1 4 Hydrogen chloride HCl concentrat ed 1 4 4 4 1 1 2 3 1 4 Hydrogen peroxide H2O2 3 1 3 1 1 1 1 1 1 3 Hydrogen peroxide H2O2 30 1 4 1 4 1 1 1 1 3 Hydrogen sulfide H2...

Page 87: ...ate AgNO3 100 1 1 1 1 1 1 1 1 1 4 Sodium bisulfite NaHSO3 10 1 1 2 4 1 1 1 Sodium carbonate Na2CO3 10 1 1 1 1 1 1 3 Sodium chloride NaCl 30 1 1 1 1 1 1 1 1 1 3 Sodium hydroxide NaOH 30 1 1 4 1 1 1 1 1...

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Page 89: ...EC Declaration of Conformity...

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Page 91: ...d 53 Brief mains power failure 60 Bucket 16 26 65 66 Buzzer 36 55 Calling up the program list 31 Calling up the rotor selection list 31 Carrier 26 66 68 Centrifugation curves 38 Centrifugation with di...

Page 92: ...10 Declaration of decontamination 70 71 Deformation of tubes 68 Deleting a program 41 58 Density 12 17 33 39 50 56 82 85 Design of the centrifuge 11 Dialog box 20 37 38 40 Dialog box for changing the...

Page 93: ...et rotors 32 47 Imbalance 17 25 26 66 Imbalance dialog box 38 Imbalance error 62 Imbalance message 36 55 Imbalance monitoring 20 38 Importance of the operating manual 9 Important information 14 Improp...

Page 94: ...peed for glass tubes 26 78 Maximum speed for tubes 78 Measures in the event of hazards and accidents 20 Mechanical safety 16 Menu bar 43 55 Modification of existing curves 38 Modification of parameter...

Page 95: ...substances 17 64 65 Radius 32 39 50 56 79 82 Rapid cooling program RAPID_TEMP 31 33 39 47 49 57 RCF 29 32 39 45 50 56 79 82 RCF field 27 43 Relative centrifugal force 29 32 45 82 Remaining hazards 20...

Page 96: ...ors and accessories 18 19 84 Service life of the accessories 68 Service request 3 63 69 Service work 69 Set 29 Set function 29 Setting the rcf value 29 Setting the runtime 29 Setting the speed 29 Sett...

Page 97: ...erature 30 39 45 56 Temperature control 51 Temperature display 51 Temperature error 62 Temperature field 27 43 Temperature fuses 23 Temperature profiles 39 57 Temperature range 72 Temperature unit 54...

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Page 99: ...knob Total view of the centrifuge 8 Name plate 9 Mains power input 10 Equipotential bonding screw Rear view of the centrifuge 1 Before the initial start up please ensure that your centrifuge is proper...

Page 100: ...e displayed one after the other Select a program and confirm the selection A dialog box will be displayed Select LOAD and confirm the selection Manual Mode Turn the function knob in order to select a...

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