Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516
3
www.promega.com
TM320 · Revised 4/15
1.
Introduction
1.A. Maxwell
®
16 MDx Purification Procedure
The Maxwell
®
16 MDx Instrument
(a)
provides automated nucleic acid purification methods for multiple analytes.
The methods use sample lysis and binding to paramagnetic particles as the primary separation principle. It has
two modes for di
ff
erent elution volumes. The Standard Elution Volume (SEV) mode allows elution in a volume of
up to 400µl. The Low Elution Volume (LEV) mode allows elution in as little as 25µl. Up to 16 samples can be
prepared in a single run.
The automated steps performed by the Maxwell
®
16 MDx Instrument include:
•
Sample lysis in the presence of a chaotropic agent, detergent and/or alcohol
•
Binding of nucleic acids or proteins to paramagnetic particles
•
Washing of the target molecules bound to the particles away from other cellular components
•
Elution of the product
The instrument contains a touch screen for navigating, programming and running the instrument. It has a UV
lamp to aid with decontamination. The Maxwell
®
16 MDx Instrument, in association with the Maxwell
®
Sample
Track Software, has the ability to record and provide run data. It has a USB port that can be used to attach a bar
code reader, allowing sample and reagent information to be entered using bar codes. The Maxwell
®
16 MDx
Instrument can report the data gathered for each run, and the report can be sent to a serial printer or to a
computer.
The user selects the protocol to be run, collects the selected bar code information (optional) and places the
samples into the reagent cartridges. The cartridges are placed into the machine and the door shut to start the run,
which automatically performs the protocol.
The temperature of the samples is regulated by a heating system that is controlled by the protocol.
Maxwell
®
Sample Track is a communications program that allows downloading and printing of the run data
(see Technical Manual #TM314).
