52
Promega Maxwell
®
CSC 48 Instrument | TM623 | Revised 6/20
6.2 Starting a Method (continued)
Important:
The plungers must be placed in the well closest to the elution tubes. If the
instrument goes through a run with the manetic rods unprotected, the magnetic rod assembly
must be thoroughly cleaned (see Section 7.2, Cleaning the Hardware), and the cartridges
containing samples discarded. The samples will be lost.
Figure 41. Placing the deck tray in the instrument.
6. If the Vision system is active on your system, when the deck tray is retracted after pressing
Start
, the Vision system will scan the deck trays to confirm deck tray setup. The Vision
system will check that cartridges are present at all active cartridge positions, plungers are
present at well #8 in the cartridges, elution tubes are present and open for every cartridge
position. If there are any discrepancies in deck tray setup, a 'Machine Vision Error' screen is
displayed indicating that errors need to be resolved before the extraction run can proceed.
Press the
Door Open
button to extend the deck tray and return to the 'Cartridge Setup'
screen, or press
Cancel
to return to the 'Cartridge Setup' screen without extending the
deck tray. On the 'Cartridge Setup' screen, any positions of concern will be noted with an
exclamation mark in a red circle. The
Front
and
Back
buttons on the screen will also
display the exclamation mark in a red circle to indicate if there are issues on either deck tray.
Touch the exclamation mark in the red circle at any cartridge position to display a message
indicating the issues that were observed by the Vision system.
Resolve all issues with the cartridge setup on the deck trays. After all issues have been resolved,
touch the
Proceed
button to rescan the deck trays and start the run.
7. While the extraction method runs, you will see the ‘Running’ screen (Figure 42). The title bar
of the ‘Running’ screen indicates the method currently being run. This screen displays:
• The name of the user who started the method run.
• An estimate of the time remaining until the end of the run.
• A description of the current step being performed.
• A progress bar showing the percent completion of the method.
It is possible to perform some instrument functions (e.g., viewing reports) during an
extraction run by touching the
Home
button and selecting a function. Touch the rotating
timer icon in the title bar from any other screen to return to the ‘Running’ screen while a
method is running. If you wish to abort the current run, touch the
Abort
button in the lower
right corner of the screen.
Operating the Maxwell® CSC 48 Instrument