51
Promega Maxwell
®
CSC 48 Instrument | TM623 | Revised 6/20
After performing each step touch the
Confirm
button next to that step to indicate that it has
been performed. Only after all of the checklist items have been confirmed will the
Start
button
become active (Figure 40). Touch the
Start
button to begin the purification or touch the
Cancel
button to return to the ‘Cartridge Setup’ screen.
16980T
A
Figure 40. Completed Extraction Checklist.
When all checklist items have been confirmed,
the
Start
button will become active. Touch the
Start
button to begin the processing run or the
Cancel
button to return to the sample information entry screen.
• Completely remove seals from all cartridges.
• Place elution tubes at the selected positions in the deck trays.
• Add the appropriate volume of elution buffer to each elution tube (see the Maxwell
®
CSC
reagent kit Technical Manual for the correct volume to use). Leave the elution tube lids open.
• Add preprocessed sample to well #1 (largest well) of the cartridge.
• Ensure no plungers from the previous run(s) are present on the plunger bar inside the
instrument. If there are plungers present, go to Clean Up in Section 5.2, User Interface
Settings, for instructions for plunger removal.
• Place a plunger in the last well (closest to the elution tube) of each cartridge.
• Place the deck trays in the instrument with the
Back
tray on the back deck position and
Front
tray on the front deck position as shown in Figure 41. The deck trays for the
instrument are keyed so that they will only fit in their appropriate deck position within the
instrument. Hold the deck tray by the sides to avoid dislodging cartridges from the deck
tray. Ensure that the deck tray is placed in the Maxwell
®
Instrument with the elution tubes
closest to the door. Angle the back of the deck tray downward and place into the instrument
so that the back of the deck tray is against the back of the instrument platform. Press down
on the front of the deck tray to firmly seat the deck tray on the instrument platform. If you
have difficulty fitting the deck tray on the platform, check that the deck tray is in the correct
orientation. Ensure the deck tray is level on the instrument platform and fully seated. After
all of these steps have been performed, touch the
Start
button to begin the purification, or
touch the
Cancel
button to return to the previous screen.
Important:
The
reagent cartridges
are designed to be
used with potentially
infectious substances.
Wear the appropriate
protection (i.e.,
gloves, goggles,
etc.) when handling
infectious substances,
and adhere to your
institutional guidelines
for the handling
and disposal of all
infectious substances
used with this system.
Operating the Maxwell® CSC 48 Instrument