4
Promega Maxwell
®
CSC 48 Instrument | TM623 | Revised 6/20
1.1 Maxwell
®
CSC 48 Instrument Purification Procedure
The Maxwell
®
Clinical Sample Concentrator (CSC) Instruments provide automated nucleic acid
purification for a range of sample types. The Maxwell
®
CSC 48 Instrument is intended for use by
laboratory professionals. The purification methods use sample lysis and binding to paramagnetic
particles as the primary separation principle. Up to 48 samples can be prepared in a single run.
The automated steps performed by the Maxwell
®
CSC 48 Instrument include:
• Sample lysis in the presence of a specially formulated Lysis Buffer
• Binding of nucleic acids to paramagnetic particles
• Washing of the bound target molecules away from other cellular components
• Elution of the product
The instrument is controlled through a graphical user interface running on a Tablet PC. The
Maxwell
®
CSC 48 Instrument has the ability to record and report sample tracking and method
run data. A bar code reader is supplied with the Maxwell
®
CSC 48 Instrument and is used
for selecting methods and capturing bar code information for samples and reagents. The
Maxwell
®
CSC 48 Instrument provides reports of the data gathered for instrument operations;
reports can be printed and exported to a storage location or a USB drive for transfer to a
separate computer. To start a run, the user scans the method bar code of the reagent kit to be
processed. After entering the sample tracking information, follow the recommended protocol
for the Maxwell
®
CSC reagent kit and prepare the deck trays of the instrument as instructed.
Place the deck trays containing prepared cartridges into the instrument, and the method will
run automatically. Using the instrument does not require any special training; however, training
is available as part of Operational Qualification (OQ; see Section 10.2).
Introduction
1
