PerkinElmer Wallac 1420, User Manual

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CW-Lamp energy
Using this parameter you are able to change the excitation energy supplied by the CW-
Lamp.
CW-Lamp Control Mode
There are two ways of ensuring that the light incident on the plate from the CW-Lamp
remains constant – stabilized energy mode or constant voltage mode. In the stabilized
energy mode a photodiode close to the light path has part of the incident light directed onto
it. This sends feedback to the lamp controller which alters the lamp voltage to adjust the
intensity of the light produced so that it remains constant. In the constant voltage mode the
lamp voltage is not changed but the software corrects for changes in the lamp output. You
can select the control mode you want from the option buttons. The default mode is
stabilized energy. The use of the constant voltage mode allows the CW-Lamp lamp to be
run continuously at maximum power and it is faster than the stabilized energy mode. This
enables optimum performance in cases where the light intensity is critical, as in
fluorescence polarization or where speed is critical as in fast kinetics.
CW-Lamp filters
You can select the CW-lamp excitation filter from the drop-down list of available filters.
See the chapter on Filters for more information about how to define filters.
Factory pre-set CW-lamp filter names begin with a letter indicating the technology for
which they are to be used:
F is for fluorometry. After the letter comes the wavelength.
Excitation aperture (Wallac 1420-040/041/042/043 only)
There are three sizes of excitation aperture controlling the size of the incident light beam.
You can select with the option buttons Small, Normal or Large. Normal is the default.
Emission filters
You can select the emission filter from the drop-down list of available filters. See the
chapter on Filters for more information about how to define filters.
Factory preset emission filter names begin with a letter indicating the technology for which
they are to be used:
F is for fluorometry. After the letter comes the wavelength.