PerkinElmer Wallac 1420, User Manual

Page: 118 / 171

Tools menu
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The window that appears allows you to define the centre point of each of the four corner
wells.
Each tab shows one of the four wells with a plot of the intensity of the light from the well.
This is colour coded with red showing the highest intensity. Position the mouse cursor
where you think the centre of the well is and click the left mouse button. A black cross will
appeared centred at the point of the cursor. If you are not satisfied with the position of the
cross move the mouse cursor and click the left button again.
Repeat this for each of the four wells.
When the centre of all four wells has been marked, the Next button will be enabled. Click
this to continue.
Numerical values are shown in the final window. If you approve these, click Finish to save
the values and end the wizard. If you feel the deviations are too large you can click Back to
reposition the well centres. You can alternatively click Cancel to end the wizard without
saving the values.
Note: for even better adjustment it is recommended that you repeat the scan with a smaller
area selected, see the lower figure on page 112, e.g. for a 1536-well plate this could be 1
mm per well.