PerkinElmer Wallac 1420, User Manual

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Filter properties
The filters used in the instrument have the properties shown in the table below.
The following abbreviations are used:
CWL = Centre wavelength, BW = Bandwidth (Full Width Half Maximum), Tpk =
Maximum transmittance at the peak wavelength (%)
Filter type Name Centre
Wavelength
(CWL) Bandwidth
(BW) (Full
Width Half
Maximum) Maximum transmittance
at peak wavelength (Tpk)
(%)
Tr-fluorescence excitation D340 340 nm, ca 50 nm ca 60%
Tr-fluorescence excitation D320 320 nm, ca 80 nm ca 70%
Europium chelate emission
(LANCE option installed) D615 615 nm, ca 8.5 nm ca 80%
Europium chelate emission
(LANCE option installed) D665 665 nm ca 7.5 nm ca 80%
Samarium chelate emission D642 642 nm, ca 6.5 nm ca 80%
Terbium chelate emission D545 545 nm, ca 7.5 nm ca 70%
Dysprosium chelate emission D572 572 nm, ca 7.5 nm ca 70%
Colorimetric label emission P405 405 nm, ca 10 nm ca 45%
Colorimetric label emission P450 450 nm, ca 6 nm ca 30%
Colorimetric label emission P490 490 nm, ca 6 nm ca 30%
Fluorescein excitation F485 485 nm ca 15 nm ca 60%
Fluorescein emission F535 535 nm ca 25 nm ca 50%
Umbelliferone excitation F355 355 nm ca 40 nm ca 35%
Umbelliferone emission F460 460 nm ca 25 nm ca 50%
Note: the time-resolved filters are available only if this option is included with the
instrument.