PerkinElmer Wallac 1420, User Manual

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Counting parameters
These determine the time factors involved in the measurement process, all times are in
microseconds:
Counting delay - the time after the excitation pulse at which counting of the emission
signal begins
Counting window - the time period within which counting occurs
Counting cycle - the time between excitation pulses within a measurement.
The second or additional counting window is fully independent of the first one and it useful
when optimizing time-resolved parameters or for calculations of the decay time of the label.
Factory-set values for the main counting window
Label
Delay 1 Delay 2 Window 1 Window 2 Cycle
High count 615 50 0 100 0 1000
High count 665 50 0 100 0 1000
Long decay 615 50 0 100 0 3000
Long decay 665 50 0 100 0 3000
545 50 0 100 0 3000
615 50 0 100 0 1000
665 50 0 100 0 1000
572 50 0 100 0 3000
TruPoint 615 50 200 100 100 1000
TruPoint 545 50 200 100 100 1000
Beam size
This parameter can be selected to be Normal or Narrow provided the Adjustable beam size
option has been installed. It enables measurements on plates with very small well sizes by
focusing the excitation light beam to a smaller size.
Second measurement parameters
In the case where you want to make measurements with two filters, you can use this
parameter to select the type of the second emission filter. The options are the same as for the
first measurement emission filter. Two measurements are made, first with the normal filter
and then with the second, which the system automatically moves into place.