PerkinElmer Wallac 1420, User Manual

Page: 48 / 171

Protocol editing
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the number of replicates (from 1 to 3)
the injection mode . You can either aspirate and then dispense the same amount
(aspVol=dispVol) or you can fill the whole syringe and then dispense several times
(aspVol=syringeVol). The former is more accurate but the latter is faster.
At the bottom of the dialogue is a check box “Perform on first plate repeat only”. If you
only want dispensing for the first of the plate repeats, check this box. If you want
dispensing for every repeat then leave it unchecked. Clicking the
OK button inserts a
dispense operation in the measurement operations list.
Delay button
Clicking this causes a dialogue to appear that lets you specify the duration of the delay
between the completion of the preceding operation and the start of the one succeeding the
delay operation in the operation list. You use a slide bar to do this. Clicking the
OK button
inserts the delay operation into the measurement operations list. A single delay can be from
0.1s to 3600 s.
At the bottom of the dialogue is a check box “Perform on first plate repeat only”. If you
only want delay for the first of the plate repeats, check this box. If you want delay for every
repeat then leave it unchecked.
Shake button
Clicking this causes a dialogue to appear that lets you specify the parameters for a shake
operation. You can specify the duration of the operation, the speed (slow, normal or fast),
the extent the shaker moves and the path that it follows - straight line, elliptical or figure of
eight (linear, orbital or double orbital respectively). Clicking the
OK button inserts the
shake operation into the measurement operations list.
At the bottom of the dialogue is a check box “Perform on first plate repeat only”. If you
only want shaking for the first of the plate repeats, check this box. If you want shaking for
every repeat then leave it unchecked.
Label button
Opens the Label Selector to let you specify a label to be inserted in the measurement
operations list. There can be up to 10 labels in the list. Note: for LANCE measurements two
labels must be selected. If only one is selected then the measurement will be treated as
normal time-resolved fluorescence.
When using well or strips mode, the label button will be disabled when there is already a
label in the measurement sequence. However, copying and pasting of the same label is
possible. For detailed information about labels see Labels on page 67.