PerkinElmer Wallac 1420, User Manual

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Appendix 3: Dispenser operation
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Appendix 3: Dispenser operation
Introduction
The optional dispenser allows you to make measurements that require e.g. the addition of
reagent to start the process, as in the case of flash luminescence, or start and stop an enzyme
reaction. It can also be used for dispensing Enhancement Solution for a DELFIA time-
resolved fluorometry measurement. It can be used with any of the technologies supported
by Wallac 1420.
The dispenser can have up to four pumps fitted. This allows you to have separate pumps
dedicated for specific purposes. All the needles are directed to the same well allowing
dispensing of more than one reagent to the same well. The number of pumps fitted and the
total volume of each pump are set during installation. This information then appears when
dispenser maintenance is selected. The default setting for the dispensed volume can be
between 5 to 350 microlitres.
Note: dispensing to 384-well plates is possible if just one pump is installed.
The optional dispenser is a separate unit from Wallac 1420 itself making for convenient
maintenance and flexibility in syringe selection.
The information about installing the dispenser unit will be found in the Instrument manual
as part of the installation instructions.
The following instructions tell how to use the dispenser.