46
Note
►
Make sure that a Chamber Calibration has been performed (see paragraph 8.2.5.1).
A distinction should be made between the
Chamber Control
mode and the
Sample Control
mode.
To start the Obj. Heater calibration in
Chamber Control
mode, follow the instructions below:
•
Make sure that T Sensor is connected to UNO-CONTROLLER (see paragraph 7.4).
•
Secure with commercial adhesive tape the T Sensor to the bottom of the sample holder you use during
standard operation (Petri dish, Chambered Glass Slide, etc.) as shown in Figure 66. Do not cover the
tip of the T-Sensor with the adhesive tape.
Figure 66. T Sensor attached on the bottom of the Petri dish (a -b).
Tip
►
Use the same dish that you use in regular practice.
•
Fill the sample holder with water or oil to the level used during standard operation. Make sure the tip
of the T Sensor is immersed in the liquid. Cover the sample holder with the lid or leave it open,
according to the protocol followed during standard operation.
•
Close the incubating chamber with its lid.
•
Make sure that the microscope objective heater is equipped with the OBJ-COLLAR. Make sure that the
Objective Heater touches the sample in both oil immersion and water dipping imaging.
•
Press
Calibrate
button (see pointer 5 in Figure 62 b) to start the Obj. Heater calibration procedure
(see Figure 56 b). A pop-up message will appear warning you that the Chamber calibration must be
performed before the Obj. Heater one. Make sure that the Chamber calibration has been already
performed and click
OK
to procced. Click on
Abort
button to interrupt the Obj. Heater calibration
procedure (see Figure 56 b).
Tip
►
If you need to perform the Chamber calibration, you can keep the Objective Heater enabled. Make sure
that the Objective Heater does not touch the sample during the Chamber Calibration procedure.
