Okolab UNO-T Manual Download Page 37

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8.2.2.1

Compatible Okolab H301 incubating chamber List

Table 4 reports all the available Okolab H301 incubating chambers for UNO-STAGE-TOP-INCUBATOR.

Chambers:

H301-K-FRAME
H301-NIKON-TI-S-ER

H301-OLYMPUS-IX3-SVR
H301-PRIOR-H117

Detail:

Fits standard XY stages with k-frame insert (160x110mm).

Chambers:

H301-LUDL96A602
H301-MCL-Z100/500
H301-NIKON-NZ100/200/500-N
H301-PI-736-160x110

Detail:

Fits Z-piezo stages models listed.

Chambers:

H301-MINI

Detail:

Fits in any MW plate holder.

Chambers:

H301-NIKON-TI-SR
H301-OLYMPUS-IX-SUSP

Detail:

Fits rectangular stages with circular opening.

Chambers:

H301-EC-LG-BL

Detail:

Fits Leica Super Z Galvo Stage (SP5/SP8).

Chambers:

H301-PRIOR-NZ500

Detail:

Fits Prior Z-piezo stages models NZ250/400/500.

Summary of Contents for UNO-T

Page 1: ...UNO STAGE TOP INCUBATOR UNO T UNO T H PREMIXED UNO T H CO2 Manual IST872_REV07 SV 1 6 1 0 ...

Page 2: ...This page was left Blank ...

Page 3: ...OPTIONAL 20 MINI USB PORT 21 7 6 1 Connection of MINI USB OTG cable 21 7 6 2 Connection of MINI USB cable 21 RS232 SERIAL PORT 22 8 USER INTERFACE 24 HOME PAGE 24 8 1 1 How to enter the Setpoint 25 8 1 2 Graph view 25 8 1 3 Controller Status colours led and meaning 26 SETTINGS 27 8 2 1 Control Mode 27 8 2 1 1 Chamber Control mode 28 8 2 1 2 Sample Control mode 29 8 2 2 Chamber Insert 31 8 2 2 1 Co...

Page 4: ...2 8 9 Auto on off 57 STATUS OVERVIEW 59 ICON AND GLANCE MODE VIEW 59 INFO PAGE 59 9 TOUCH SCREEN CALIBRATION 61 10 CLEANING MAINTENANCE 62 11 SUPPORT 63 12 TECHNICAL SPECIFICATIONS 64 13 TROUBLESHOOTING 66 14 FIGURE LIST 67 15 TABLE LIST 68 16 MANUAL REVISION TABLE 68 ...

Page 5: ...sired output gas flow rate in the range 0 1 0 4 l min by regulating manually the pressure o GF MIXER HM the Humidity Module Dry premixed gas is humidified by flowing over warm water heated up by the Humidifier The Humidifier is composed of a glass bottle Humidifier Bottle and its heater A heated tube delivers the humid premixed gas to the stage top incubator preventing moisture condensation UNO T ...

Page 6: ...actices that can affect the functionality of the instrument Tip Supplies you with helpful suggestions Note Supplies you with important information to successfully use the instrument Symbols on the product label CE MARKING this symbol indicates product compliance with EU legislation PRODUCT DISPOSAL this symbol indicates that this product must not be disposed as urban solid waste This symbol indica...

Page 7: ...ream whose composition is different from that of ambient air Prevent throttling and kinking of tubing Check tubing time to time for possible material usage Check that all tubing ends are well inserted into the connectors so they cannot slip off This device is not designed for use for medical applications Install safety valves and adequate pressure regulators on gas lines before the unit input conn...

Page 8: ...here it can fall or be pushed into water International caution symbol marks this device It is important to read the Safety Notes before installing using and commissioning this device as the notes contain important information relating to safety and EMC Not following these instructions can result in damage or breakdown of the device and its accessories We reserve the right to make technical variati...

Page 9: ... extension x1 1 4 3 5 2 6 7 Figure 1 UNO T Equipment Overview UNO T H PREMIXED Figure 2 illustrates the equipment overview for UNO T H PREMIXED configuration 1 UNO CONTROLLER x1 temperature controller 2 Y CABLE 2 x1 To connect the Humidifier and the Heated tube to the temperature controller 3 Y CABLE 1 x1 To connect chamber s base and lid to the temperature controller 4 24V DC Power Adapter x1 5 M...

Page 10: ...incubating chamber 12 Okolab H301 incubating chamber required to be ordered separately 13 OBJ COLLAR optional 1 4 6 2 5 8 9 12 13 3 To premixed gas supply 11 7 10 Figure 2 UNO T H PREMIXED Equipment Overview UNO T H CO2 Figure 3 illustrates the equipment overview for UNO T H CO2 configuration 1 UNO CONTROLLER x1 temperature controller 2 Y CABLE 2 x1 To connect Humidifier and the Heated tube to the...

Page 11: ...ressure gauge to the CO2 input port on the rear panel of 2GF MIXER 12 TUBE C x1 Blue rigid tube 6mm O D connected to a 4mm I D silicon transparent with a PTFE filter in the middle Use TUBE C to connect the gas output of the GR REGULATOR to the gas input connector of Humidifier 13 Humidifier x1 It is composed of a glass bottle Humidifier Bottle with TUBE E 6 mm OD silicon tube with a glass bubbler ...

Page 12: ...th heated lid and base chamber riser with one or more plate adapters Refer to paragraph 8 2 2 1 for the complete list of compatible H301 incubating chamber OBJ COLLAR series optional recommended for immersion objectives DATA LOG optional Software used to log view and store data from a PC It may be used to change parameters of the controllers via PC Data can be exported in xlsx format Figure 4 DATA...

Page 13: ...LLER Left Panel Overview Figure 6 illustrated UNO CONTROLLER rear panel 3 Power input 4 Base Lid connector To provide power to the Chamber Base and Chamber Lid 5 Humidifier Heated Tube connector To provide power to Humidifier and Heated Tube 6 Obj Heater connector To provide power to OBJ COLLAR 7 T Sensor connector 3 4 5 6 7 Figure 6 UNO CONTROLLER Rear Panel Overview Figure 7 illustrates UNO CONT...

Page 14: ...10 8 9 Figure 7 UNO CONTROLLER Front Panel Overview ...

Page 15: ...d Chamber Base UNO CONTROLLER Y CABLE 1 WP CABLE WP CABLE Figure 8 Chamber Base and Chamber Lid Connection Tip Connecting cables are equipped with identification tags to guide you through the installation process Note If the Base cable or the Lid cable is equipped with a DIN connector attach it to its corresponding connector of Y CABLE 1 Connection of GF MIXER HM Humidity Module This section descr...

Page 16: ...nnector Figure 9 Humidifier Assembly Connect the rigid end of Heated Tube to the Gas Outlet connector of Humidifier Connect the silicon end of Heated Tube to the gas input connector located on a corner of Okolab H301 incubating chamber see Figure 10 Tip Make sure to push the tubes all the way into the connectors thus avoiding any gas leak ...

Page 17: ... UNO CONTROLLER 2 Connect the GREEN labeled Humidifier Heater cable of the Humidifier to its corresponding connector of Y CABLE 2 3 Connect the Heated Tube cable to its corresponding connector of WP CABLE Connect WP CABLE to the GREY labeled connector of Y CABLE 2 Humidifier Heater Heated Tube UNO CONTROLLER Y CABLE 2 WP CABLE Figure 11 GF MIXER HM Connection Tip Connecting cables are equipped wit...

Page 18: ...e Figure 12 a b Figure 12 GF REGULATOR front and rear view a b The premixed gas tank must be equipped with a pressure regulator to decrease the pressure from the pressure tank to 4 barg 58 psig Do not exceed 10 barg 145 psig for GF REGULATOR inlet gas pressure This may damage the GF REGULATOR 2 Connect the blue rigid end of TUBE C to the output connector of GF REGULATOR Connect the silicon end of ...

Page 19: ... between pressure and the gas flow rate for the GF Regulator Pressure barg Gas Flow Rate l min 0 2 0 17 0 4 0 22 0 6 0 27 0 8 0 32 1 0 0 37 1 2 0 41 Table 1 GF REGULATOR Pressure vs Gas Flow Rate Tip If the desired gas flow rate is 0 27 l min the pressure of the GF REGULATOR has to be set at 0 6 barg 8 7 psig Tip Okolab suggests setting the gas flow rate in the range 0 3 0 4 l min If the total con...

Page 20: ...of the arrow on the rear of the pressure gauge for the correct gas in out orientation The CO2 tank must be equipped with a pressure regulator to decrease the pressure from the pressure tank to 2 barg 29 psig Do not exceed 10 barg 145 psig for the pressure gauge inlet gas pressure This may damage the pressure gauge 2 Use TUBE A to connect the output connector of the pressure gauge to the CO2 In pus...

Page 21: ...nd High Flow in the range 0 5l min 1 0 l min Slide to the right the manual switch placed on the bottom panel of OKO AP to select the High Flow mode see image A in Figure 16 Slide to the left the manual switch to select the Low Flow mode see image B in Figure 16 A B Figure 16 OKO AP Low Flow and High Flow Note To switch OKO AP on use the Power switch on the rear panel see Figure 17 Figure 17 OKO AP...

Page 22: ...F MIXER generates a CO2 air mixture with an adjustable CO2 concentration in the range 0 15 The desired CO2 concentration is obtained by regulating the air and CO2 flow rate in the range 0 1 1 l min and 0 013 0 13 l min respectively using the two floating ball flow meters Do not use the 2GF MIXER as closing opening valves Once regulated the flow rate at inlet CO2 pressure of 1barg use the 2GF MIXER...

Page 23: ...dium evaporation normally it decreases if the outlet total gas flow rate decreases If the desired outlet total gas flow rate is 0 84 l min the air flow rate has to be set at 0 80 l min and the CO2 flow rate at 0 04 l min 3 Flow rate regulation means turning the knob until the floating ball is placed in correspondence of the desired value see Figure 19 Notice that the correct position of the floati...

Page 24: ...gs UNO CONTROLLER s green connector Insert the widest connector pin of T Sensor into the bottom opening of UNO CONTROLLER s green connector Connection of the OBJ COLLAR optional To connect the OBJ COLLAR to UNO CONTROLL follow the instructions below 1 Assembly the OBJ COLLAR around the objective heater see Figure 21 Objective Band Heater 1 2 Objective Band Heater Velcro Tape Figure 21 OBJ COLLAR A...

Page 25: ...I USB provides the connection for the supplied MINI USB OTG cable or a standard MINI USB cable 7 6 1 Connection of MINI USB OTG cable Use the supplied MINI USB OTG cable to connect a USB pen drive not supplied to UNO CONTROLLER as shown in Figure 23 USB Pen Drive MINI USB OTG cable Figure 23 MINI USB OTG cable Connection 7 6 2 Connection of MINI USB cable Use a standard MINI USB cable to connect U...

Page 26: ...d RS232 serial cable to connect UNO CONTROLLER to a PC as shown in Figure 25 This option is advisable when the PC is at a distance greater than 10 meters from UNO CONTROLLER You can interface with UNO CONTROLLER via PC by using DATA LOG software The software allows to log and analyze the data provided by UNO CONTROLLER via PC Refer to DATA LOG user manual for more info SDK OKOLAB API for integrati...

Page 27: ... to install the Serial Communication cable to your PC you need to install a USB to serial converter following the manufacturer instructions If you want to log you have to use the COM port number that Windows assigned to the drive ...

Page 28: ...en UNO CONTROLLER is connected to a PC using a standard MINI USB cable 8 Open Close Incubator icon Open incubator feature is available for Sample Control mode see paragraph 8 2 1 2 9 Support To open Support page 10 Control mode indicator It reports Sample Control mode or Chamber Control mode see paragraph 8 2 1 11 Status indicator see paragraph 8 1 3 12 Temperature Set point See paragraph 8 1 1 to...

Page 29: ...change the Temperature Setpoint a b Tip After any change in the Setpoint value UNO CONTROLLER enters into a transient regime the Status Indicator and the TOP LED see Figure 29 turn yellow see paragraph 8 1 3 During the transient regime UNO CONTROLLER will not trigger on alarm The maximum duration of the transient regime is set by the operator as indicated in paragraph 8 2 7 8 1 2 Graph view Press ...

Page 30: ...hin the maximum time defined by the operator see paragraph 8 2 7 the Status changes to ALARM and the color turns ORANGE Controller Status TRANSIENT The ORANGE color indicates that the current temperature value is not correct and its value is out of the set tolerance see section ALARMS in paragraph 8 2 7 Most commonly this is related to gas leaks or gas source s running low Verify that all cables a...

Page 31: ...ettings menu a b Press the arrow in the bottom right corner of the page see Figure 31 a to open the second page of the Settings menu see Figure 31 b a b Figure 31 Settings menu a b 8 2 1 Control Mode Press on Control Mode icon to enter the Control Mode menu see Figure 32 a b Figure 32 How to enter the Control Mode menu a b ...

Page 32: ...perature is maintained at the desired set point value Note The Chamber Control mode affords a quick experiment start No further actions are required for multi user applications Tip In Chamber Control mode there is no need to place the T Sensor the fine gauge thermocouple into a reference well during experiment After selecting Chamber Control mode the screen in Figure 34 b will appear a b 1 2 Figur...

Page 33: ...res that the temperature sample differs by not more than 0 1 C from the temperature set point regardless from the Room Temperature s variations The disadvantage of this control is that it requires manual operation for the correct placement of the T sensor in a reference well In Sample Control mode while running the experiment the T Sensor needs to be placed into a dish Petri dish Chambered Glass S...

Page 34: ...SENSOR LID CODES SENSOR LID 35 Temperature sensor lid To be used in specimen feedback in a 35 mm Petri Dish SENSOR LID 60 Temperature sensor lid To be used in specimen feedback in a 60 mm Petri Dish SENSOR LID GS Temperature sensor lid To be used in specimen feedback in chamber slides and chambered cover glass Table 3 Sensors lid codes The Sample Control mode provides the Open Incubator feature Du...

Page 35: ...rt icon to enter the Chamber Insert menu see Figure 39 a b Figure 39 How to enter the Chamber Insert menu a b Here you have to set the model of Okolab H301 incubating chamber that you have purchased and the type of sample adapter being inserted into it Depending on these selections UNO CONTROLLER will automatically change the control settings To set the configuration press on the Chamber icon see ...

Page 36: ... a b 1 2 3 Figure 41 Settings Chamber Insert Insert selection a b Tip Chambers equipped with MW OIL insert have been factory calibrated at a Room Temperature of 23 C 1 C for a temperature set point of 37 C The offset of the Chamber Base and the Chamber Lid has been optimized to guarantee a uniform temperature for all the wells Okolab suggests operating using factory offset values To view factory o...

Page 37: ...with k frame insert 160x110mm Chambers H301 LUDL96A602 H301 MCL Z100 500 H301 NIKON NZ100 200 500 N H301 PI 736 160x110 Detail Fits Z piezo stages models listed Chambers H301 MINI Detail Fits in any MW plate holder Chambers H301 NIKON TI SR H301 OLYMPUS IX SUSP Detail Fits rectangular stages with circular opening Chambers H301 EC LG BL Detail Fits Leica Super Z Galvo Stage SP5 SP8 Chambers H301 PR...

Page 38: ...s the usage of dipping lenses Chambers H301 DUAL GAS Detail Chamber with two independent gas compartments Chambers H301 ALPHA3 SMALL Detail Incubating chamber for PhaseView Alpha3 system small version Chambers H301 ALPHA3 LARGE Detail Incubating chamber for PhaseView Alpha3 system large version Chambers H301 MIZAR TILT Detail Incubating chamber for Mizar Tilt modular Light Sheet system Chambers CU...

Page 39: ...g rate in C min for the Objective Heater and press Save to confirm a b 1 2 Figure 44 Settings Obj Heater Ramp rate a b Tip It is particularly indicated to use a low heating rate in case of Objective Heater acting on a very sensitive objective Check with the objective manufacturer Note The ramp rate can be defined in the range 0 1 1 5 C min Above 1 5 C UNO CONTROLLER attributes the value MAX to the...

Page 40: ...lly see paragraph 8 2 5 3 4 By pressing Calibrate button see pointer 4 in Figure 43 b UNO CONTROLLER will perform a calibration routine for your specific environment see paragraph 8 2 5 3 Note To disable the Objective Heater press on the Disabled icon see pointer 1 in Figure 46 a and press OK to confirm see pointer 1 in Figure 46 b a b 1 2 Figure 46 Settings Obj Heater Disabled a b 8 2 4 Humidity ...

Page 41: ... act on the Humidity Module Offset if the desired humidity level cannot be achieved The humidity level of 90 of Relative Humidity may not be guaranteed if the Room Temperature differs by more than 1 C from 23 C and the GF MIXER HM Humidity Module is installed near heating and air conditioning vents or near windows and doors that are frequently open By pressing the Offset button see pointer 1 in Fi...

Page 42: ...ntrol enabled see pointer 1 in Figure 50 and press OK to confirm see pointer 2 in Figure 50 1 2 Figure 50 Settings Humidity Disabled 8 2 5 Calibrations Press on Calibration icon to enter the Calibrations menu see Figure 51 a b Figure 51 How to enter the Calibrations menu a b The Calibrations menu shows three options ...

Page 43: ...Sensor see paragraph 8 2 5 2 3 Obj see pointer 3 in Figure 52 Here you can perform the calibration of the Objective Heater see paragraph 8 2 5 3 8 2 5 1 Chamber Press on Chamber icon to enter the Chamber calibration submenu see Figure 53 a b 1 2 3 Figure 53 Settings Calibrations Chamber a b At this point you can choose 1 By pressing Log button see pointer 1 in Figure 53 b you can select parameters...

Page 44: ...r a temperature set point of 37 C Use factory calibration parameters if your Room Temperature is 23 C 1 C 3 By pressing Calibrate see pointer 3 in Figure 53 b UNO CONTROLLER will start a routine to calibrate its parameters using the T Sensor Calibrate UNO CONTROLLER if you Room Temperature differs by more than 1 C from 23 C To start the chamber calibration follow the instructions below Make sure t...

Page 45: ...g chamber with its lid Press Calibrate button see Figure 56 a to start the Chamber calibration procedure see Figure 56 b Click on Abort button to interrupt the procedure see Figure 56 b a b Figure 56 Settings Calibrations Chamber Calibrate a b By clicking Options see pointer 1 in Figure 57 a the menu Advanced calibration parameters will appear see Figure 57 b and here it is possible to set the cal...

Page 46: ...see Figure 58 a b 1 2 4 3 Figure 58 Settings Calibrations T Sensor a b At this point you can choose 1 Press Cancel button see pointer 1 in Figure 58 b to exit the page 2 By pressing Log button see pointer 2 in Figure 58 b you can select parameters obtained by calibrations previously performed see Figure 59 b a b Figure 59 Settings Calibrations T Sensor Log a b ...

Page 47: ...nnected to UNO CONTROLLER see paragraph 7 4 Set a water bath to the temperature you use as set point for the heating devices during standard operation most of the times it is 37 C Measure water temperature by immersing the T Sensor and a certified thermometer Press on the pencil icon see Figure 61 a and insert the reading of the certified thermometer by pressing see pointer 1 in Figure 61 b Press ...

Page 48: ...igure 63 Settings Calibrations Obj Heater Log a b 2 By pressing Factory button see pointer 2 in Figure 62 b UNO CONTROLLER will recover the factory offset 3 To perform the Manual procedure follow the instructions below Note Make sure that a Chamber Calibration has been performed see paragraph 8 2 5 1 Make sure that T Sensor is connected to UNO CONTROLLER see paragraph 7 4 Secure with commercial ad...

Page 49: ...tton see pointer 3 in Figure 62 b and insert the offset value clicking on or on the Offset tab see pointer 1 in Figure 65 b to adjust the Objective Heater offset value and press on OK to save see pointer 2 in Figure 65 b The offset is defined as the temperature difference between the set point and the reading of T Sensor a b 1 2 Figure 65 Settings Calibrations Obj Heater Manual a b To verify if th...

Page 50: ... the tip of the T Sensor is immersed in the liquid Cover the sample holder with the lid or leave it open according to the protocol followed during standard operation Close the incubating chamber with its lid Make sure that the microscope objective heater is equipped with the OBJ COLLAR Make sure that the Objective Heater touches the sample in both oil immersion and water dipping imaging Press Cali...

Page 51: ...librations Obj Heater Calibrate Options a b If you operate in Sample Control mode you need to press Calibrate button and then make the Objective Heater touch the sample in both oil immersion and water dipping imaging 8 2 6 Logging UNO CONTROLLER is equipped with on board memory for data logging and storage A simple routine allows downloading data to USB drive or to PC In order to log and download ...

Page 52: ...u can access the Logging page also by pressing the activated logging icon on the Homepage as shown in Figure 71 Figure 71 How to enter the Logging menu from the Homepage 3 Press on the icon to insert the Time Interval i e the frequency with which you want to record a data point as shown in Figure 72 In this example a data point is logged every 30 seconds you can change Time Interval in the range 1...

Page 53: ...of one week 5 Write the file name suffix to be reported in the downloaded files by pressing on the keyboard icon see Figure 73 a 6 Press Save see Figure 73 b Tip With the selections as in Figure 72 and in Figure 73 the data will be downloaded on the USB drive as shown in Figure 74 a b Figure 73 Settings Logging File name suffix a b Figure 74 Downloaded files on the pen drive Note If you press on t...

Page 54: ...r on Erase if you want to delete the data see Figure 75 b a b Figure 76 Settings Logging Download to USB a b You can activate the logging also on the USB drive by flagging on USB drive see Figure 77 a a b Figure 77 Settings Logging Download to USB Reminder to connect the USB drive a b In this case UNO CONTROLLER warns you that a USB drive must be connected to the USB port see Figure 77 b and will ...

Page 55: ...w 1 Insert the alarm time i e the time for which the temperature may remain outside the allowed tolerance before the controller triggers in alarm see pointer 1 in Figure 79 b Note The alarm time range is 5 20 minutes 2 Insert the Tolerance value This value defines the allowed tolerance from the set point see pointer 2 in Figure 79 b Note The tolerance vale range is 0 1 2 0 C 3 Insert the transient...

Page 56: ...disable the visual and acoustical alarms remove the flag from the tab labeled Enabled In this case the Alarms page becomes as shown in Figure 80 Figure 80 Settings Alarms Disabled Note When disabling the alarms a possible alarm condition is shown only by a red status LED for example is a device is accidentally disconnected Note By Pressing on Log see Figure 81 a you can view all the times that the...

Page 57: ... set at 5 minutes Note Recommended value for the chart history length is 60 or 120 minutes To change the sound frequency press the icons or scroll the Buzzer frequency bar 2 in Figure 83 b then press Save 4 in Figure 83 b to confirm Note To activate a sound at each touch flag the Touch Buzzer checkbox 3 in Figure 83 b a b 1 2 3 4 Figure 83 Settings Display Options a b 8 2 8 2 Brightness Press the ...

Page 58: ...r the calibration of the touch screen will start Figure 85 Settings Display Calibration 8 2 8 4 Date Time To set Date Time follow the instructions below 1 Press the Date Time icon see Figure 86 a 2 Set the values by using the icons see pointer 1 and 2 in Figure 86 b 3 Click on Save to confirm se pointer 3 in Figure 86 b or Cancel to undo ...

Page 59: ...have selected 12 hour clock format press on am or pm button see pointer 1 in Figure 87 b when you set the time Press Save to confirm see pointer 2 in Figure 87 b a b 1 2 Figure 87 Settings Display Date and Time 12 hour format a b 8 2 8 5 Visual Effects Press on the icon to enter the Display visual effects menu see Figure 88 Settings Display Visual effects a b a a b Figure 88 Settings Display Visua...

Page 60: ...he criterion selected in TOP LED setting and the digits displayed in Glance Mode are white If you select Dynamic the colour of the TOP LED and the colour of the digits in Glance Mode follow the colour coding reported in 8 1 3 Tip To swap between Icon and Glance mode press on the icon see paragraph 8 4 8 2 8 6 Factory Reset To reset the display settings press on the icon see Figure 89 a and press S...

Page 61: ...ary icon see Figure 92 a to enter the summary page which reports technical information a b Figure 92 How to enter the Summary page a b Note This page contains data useful for technical control reasons In case you requested support from one of Okolab engineers you may be asked for some of these data 8 2 8 9 Auto on off UNO CONTROLLER can be programmed to turn on and or turn off automatically at spe...

Page 62: ...t turn on automatically see pointer 2 in Figure 94 b Every day UNO CONTROLLER will turn on automatically every day at set time see pointer 3 in Figure 94 b Specific days UNO CONTROLLER will turn on automatically at set time on selected days see pointer 4 in Figure 94 b Flag the desired days and press OK see Figure 95 Click on Save to confirm or Cancel to undo see pointer 5 in Figure 94 b a b 2 1 5...

Page 63: ...ase you requested support from one of Okolab engineers you may be asked for some of these data Icon and Glance Mode View UNO CONTROLLER features two display modalities Icon mode and Glance Mode as shown in Figure 97 a and b You can swap between the two display modalities by pressing on icon a b Figure 97 Icon Mode and Glance Mode a b Info page Press the icon to access the Info Page as shown in Fig...

Page 64: ...60 a b Figure 98 How to enter the Info Page a b Tip Please have this information handy when contacting Okolab for support ...

Page 65: ...conds to start the Display Calibration see Figure 99 While holding the button the pop up message shown in Figure 100 a appears Then tap blue calibration dots until the calibration is complete see Figure 100 b Figure 99 Display Calibration a b Figure 100 Start Touch Screen Calibration a b ...

Page 66: ...ore refilling the bottle it is important to flush excess alcohol by using distilled water Objective Heater Remove the objective heater from the microscope objective Clean the flexible heating band with a soft microfiber cloth dampened with a 70 ethyl alcohol solution Treat the surface for one minute Clean the microscope objective following the cleaning instructions provided by the microscope suppl...

Page 67: ...o contact Okolab should you need any further commercial information or technical support For HARDWARE SUPPORT sibillo oko lab com Phone 39 081 806 3470 Fax 39 081 876 4410 Mobile 39 348 96807 18 For COMMERCIAL SUPPORT WORLDWIDE lanzaro oko lab com Phone 39 081 806 2624 Fax 39 081 876 4410 Mobile 39 348 96807 17 For COMMERCIAL SUPPORT US CANADA foppiano oko lab com For COMMERCIAL SUPPORT CHINA tong...

Page 68: ... mode if the Room Temperature is stable within 1 C GF REGULATOR input gas Premixed Gas supply The premixed gas tank must be equipped with a pressure regulator to decrease the pressure from the pressure tank to 4 barg 58 psig GF REGULATOR input gas connector Push to fit connector for 6 mm O D rigid tube GF REGULATOR output gas 0 2 1 2 barg GF REGULATOR flow rate 0 1 0 4 l min Humidification Power 8...

Page 69: ...let connector Push to fit connector for 6 mm O D rigid tube Gas outlet connector Push to fit connector for 6 mm O D rigid tube OKO AP Outlet pressure 1 3 bar 19 psi OKO AP Outlet flow rate Low Flow 0 0 5 l min High Flow 0 5 1 0 l min Humidification Power 85 90 Relative Humidity if the Room Temperature is stable within 1 C at 23 C the GF MIXER HM Humidity Module is installed away from heating and a...

Page 70: ...endrive is FAT32 formatted USB pendrive is damaged Format your USB pendrive using FAT32 file system Calibration alarm events are not logged Internal memory is full a warning icon in homepage is present Erase the full memory If you need this information download it before erasing the data The device does not log the data on the internal memory but only on the USB pendrive Internal memory is full a ...

Page 71: ...ontrol Mode Sample Control mode a b 29 Figure 36 T Sensor attached on the bottom of the Petri dish a b 30 Figure 37 SENSOR LID 35 for 35 mm Petri dish 30 Figure 38 Settings Control Mode Sample Control mode Open Incubator a b 31 Figure 39 How to enter the Chamber Insert menu a b 31 Figure 40 Settings Chamber Insert Chamber selection a b 32 Figure 41 Settings Chamber Insert Insert selection a b 32 F...

Page 72: ...ure 87 Settings Display Date and Time 12 hour format a b 55 Figure 88 Settings Display Visual effects a b 55 Figure 89 Settings Display Factory Reset a b 56 Figure 90 How to enter the Password menu a b 56 Figure 91 Settings Password Change password 57 Figure 92 How to enter the Summary page a b 57 Figure 93 How to enter the Auto on off menu a b 58 Figure 94 Settings Auto on off Auto Turn On a b 58...

Page 73: ... not warranted Okolab S r l neither assumes responsibility for any omissions or errors nor assumes liability for any damage that may results from improper use of its products in accordance with information provided by Okolab S r l Okolab S r l warrants only the parts manufactured by Okolab S r l to be free of defects Okolab S r l makes no other warranties or representations of any kind whatsoever ...

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