Ocean Optics Flame-S, User Manual

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3: Operation
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Spectroscopy Application Wizards
Click this button to set up a measurement using simple step by step wizards. A large range
of applications is available.
Application Wizard Window
Dark and Reference Measurements
Dark and reference measurements are commonly used in spectroscopy.
 Dark Measurements – subtract a background signal from the spectrum. This can be
considered the removal of a constant error. Typically this is done when the light source
is off to remove any background from the ambient environment, hence the name dark.
 Reference Measurements – make the signal relative to the reference. Consider this a
normalization of the signal against a reference. Typically this is taken with a reference
sample and the light source turned on. This lets you look at the relative spectral change
compared to a reference sample.
Most often you will set up your measurement with the reference and dark through the
application wizards. The wizards will prompt you to take your reference and darks. Alternatively
you can use the quick dark and quick reference features. Once a dark and/or reference
measurement has been set, you can update it with the controls on the top bar of the view.
Quick Reference
– click to take a reference and set up a new view. After clicking it will prompt
the user to take a dark.
Quick Dark
–
click to take a dark measurement and sets up a new Quick View minus dark view.