Ocean Optics Flame-S, User Manual

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2: Installation and Setup
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Fluorescence
Fluorescence is a technique where a sample is excited with a light source and
fluorescent light emitted from the sample at a higher wavelength is measured by
the spectrometer. Typically the excitation source is applied at 90º to the sample
to minimize light from the excitation source reaching the spectrometer. Similarly
filters are used to block lower wavelength light from reaching the detector.
Spectrometers used for fluorescence typically have a large slit, sacrificing
resolution for throughput sensitivity.
Typical Fluorescence Set Up with an LED Excitation Source at 90º
Common Fluorescence Applications

Identifying proteins using fluorophores

NADH fluorescence

Remote sensing of chlorophyll

Medical diagnosis of tumors and tissue types

Detection of anti-counterfeiting tags