Ocean Optics Flame-S, User Manual

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2: Installation and Setup
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Common UV-Vis Applications
 Quantification of DNA & proteins in life science samples
 Concentration of solutions & gaseous samples
 Identification of trace gases in a mixture
Reflectance & Transmission
Reflectance spectroscopy compares the relative level of light reflected off a
sample compared with a reference (given as a percentage of the reference
spectrum at each wavelength). A reflectance standard is used to set the
reference level of 100%. Transmission is similar but compares the light
transmitted through a sample, relative to a reference, rather than reflected off it.
Typically Reflectance uses a fiber optic probe attached to a light source and a
spectrometer, but measurements can be done easily with the Flame, both in
free-space or with the SMA Adaptor accessory. Transmission setups are usually
the same as Absorbance setups.
A Reflectance Set Up with Probe, Reflectance Standard and Probe Holder
Common UV-Vis Reflectance Applications
 Diffuse and Specular Color Measurements
 Process control for Surface quality of metals
 Thin film and semiconductor metrology
Common UV-Vis Transmission Applications
 Turbidity measurements of chemical solutions
 Measuring the transmission efficiency of optics and glass