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Leica RM 2155 Rotary Microtome
Select a sectioning speed that corresponds
to the hardness of the specimen!
Always select a slow speed when sectioning
hard samples.
Select the appropriate sectioning speed.
Select sectioning mode 2 - continuous stroke - on
the control panel.
Pull the section waste tray out.
Remove the knife guard.
Release the handwheel brake.
Start trimming with the foot switch.
Stop trimming with the foot switch upon reaching
the desired sectioning plane.
Deactivate the TRIM function.
Select the appropriate section thickness or verify
the value previously selected.
Select the sectioning mode as appropriate.
Check if the selected sectioning speed is appropri-
ate.
Start sectioning with the foot switch.
Prepare the sections.
Changing the specimen or interrupting
sectioning
Prior to manipulating the knife and specimen,
or changing the specimen or knife, and dur-
ing breaks, always lock the handwheel and
cover the cutting edge with the knife guard!
Lock the handwheel brake.
Position the knife guard over the cutting edge.
Remove the specimen from the specimen clamp and
mount a new specimen to continue.
Terminating sectioning
Lock the handwheel brake.
Remove the blade from the knife holder and put it in
the receptacle at the bottom of the dispenser.
Remove the specimen from the specimen clamp.
Transfer all section waste to the section waste tray.
Empty the section waste tray.
Clean the instrument (see chapter 13 - 'Cleaning').
Turn the instrument on with the mains switch.
Always lock the handwheel prior to manipu-
lating the knife and specimen or before chang-
ing the specimen or knife!
Lock the handwheel brake.
Always clamp the specimen before the knife!
Mount the precooled paraffin block in the univer-
sal cassette clamp.
Take care when handling microtome knives
and disposable blades. The cutting edge is
extremely sharp and can cause severe injury!
Push the knife guard to the middle of the knife
holder.
Insert the blade into the knife holder and clamp.
Adjust the appropriate clearance angle (try an angle
between 0 and 3°).
Move the specimen to the rear limit (HOME posi-
tion) using the coarse feed button.
Do not attempt to clamp, approach or orient
the specimen in the retraction phase (while
the RETRACT illuminates)!
Prior to the subsequent section a specimen
advance will be effected by the retracted
value plus the selected section thickness.
This involves the risk that the specimen col-
lides with the knife!
Approach the knife holder with the knife holder base
and hand rest to the specimen as close as possible.
Orient the specimen surface towards the knife.
The handle of the handwheel must always be
centered in motorized operation!
Center the handle of the handwheel.
Activate the TRIM function.
Select the appropriate TRIM STEP setting.
11. Daily operation
Description of the individual steps using the paraffin sectioning technique
Summary of Contents for RM2155
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