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Instruction Manual

Leica RM 2155 V1.1 English 11/2000
Always keep this manual near the instrument!

Read carefully prior to operating the instrument!

Leica RM 2155

Rotary Microtome

Summary of Contents for RM2155

Page 1: ...Instruction Manual Leica RM 2155 V1 1 English 11 2000 Always keep this manual near the instrument Read carefully prior to operating the instrument Leica RM 2155 Rotary Microtome...

Page 2: ......

Page 3: ...r ticular no liability whatsoever is accepted for any fi nancial loss or consequential damage caused by or related to compliance with statements or other infor mation in this manual Statements drawing...

Page 4: ...imen 26 10 Universal knife holder base 27 10 1 Repositioning the knife holder base 27 10 2 Mounting the knife holder 28 10 3 Adjusting the clearance angle 28 11 Daily operation 29 12 Trouble shooting...

Page 5: ...check Push the emergency stop switch The sectioning motor will stop immediately E STOP red on the control panel illuminates indicat ing that the emergency stop function is activated To deactivate turn...

Page 6: ...ally blocked LOCK yellow on the control panel Fig 19 illuminates To release the brake push the locking lever 1 to the rear LOCK extinguishes The sectioning motor can then be activated again Centering...

Page 7: ...ot be removed or modified Operating the instrument Take care when handling microtome knives and disposable blades The cutting edge is extremely sharp and can cause severe injury Never leave knives and...

Page 8: ...turn the instrument on before it is completely dry Maintenance Only authorized and qualified service per sonnel may access the internal compo nents of the instrument for service and re pair Turn the i...

Page 9: ...rotome Section thickness setting 0 5 60 m from 0 5 to 1 m in 0 5 m increment from 1 to 20 m in 1 m increments from 20 to 60 m in 5 m increments Horizontal specimen feed 27 mm via step motor Vertical s...

Page 10: ...ht Basic instrument Width including handwheel 400 mm Width excluding handwheel 300 mm Depth 550 mm Overall height 290 mm Working height knife edge 100 mm Net weight 44 2 kg External control panel opti...

Page 11: ...or section thickness setting Handwheel with centering handle Orienting specimen clamping system Universal cassette clamp UCC Control panel Clamping lever for disposable blade TRIM STEP button Clamping...

Page 12: ...a motorized rotary microtome The specimen feed system with zero backlash and maintenance free cross roller guides and the step motor operated coarse feed system are located in a dustproof plastic hous...

Page 13: ...able Lightly lift the transport platform at the front Loosen the screw 6 on the underside of the trans port platform with the Allen key size 8 7 provided and remove the washer Cut off the plastic cove...

Page 14: ...ooth operation the instrument must be set up with at least 10 cm distance from walls and furniture The installation site must meet the following require ments stable vibration free laboratory table vi...

Page 15: ...hen lock automatically when released 7 1 2 Mounting the knife holder base To release turn the lever 1 counterclockwise Place the knife holder base 2 with the two clamp ing levers 3 and 4 mounted in pl...

Page 16: ...ch nor the external con trol panel is connected the instrument will be inoperational Connecting the mains cable Before connecting the mains cable check if the mains switch 3 at the rear of the instrum...

Page 17: ...elector is set to the voltage by the factory as indicated on the order form The name plate reads the power rating of the volt age selector setting A change in the setting is generally not re quired Sh...

Page 18: ...switch at the rear This is followed by a beep The instrument initializes The software version is displayed for approx two sec onds The display reads the section thickness previously se lected e g 0 5...

Page 19: ...ton is held down to move the specimen away from the knife HOME illuminates on reaching the rear limit HOME position STOP green flashes while the coarse feed button is held down to move the specimen to...

Page 20: ...y activation of CLEAR even if the display indicates the section or trimming thickness Selecting the sectioning mode Two motorized sectioning modes are available single stroke and continuous stroke Onc...

Page 21: ...left side of the microtome The control knob has a graduated collar Speed range 0 0 5 to 420 mm s Scale range 0 10 8 2 3 Emergency stop switch The red emergency stop switch is located on the front Push...

Page 22: ...ed section thickness Selecting the TRIM STEP To select the next TRIM STEP increment turn the knob clockwise to the next lock in position The selected value is indicated in the display Activating the T...

Page 23: ...6 Indication of remaining horizontal feed STOP green illuminates approx 1 mm before the specimen reaches the front limit A beep sounds for approx 2 seconds Sectioning is interrupted and the specimen...

Page 24: ...T yellow no longer illuminates When retraction is activated the display reads on To deactivate push the coarse feed button at the rear Toactivate pushthecoarsefeedbuttonatthefront Push the CLEAR butto...

Page 25: ...l stop automatically after each section in the upper limit Sectioning mode 2 Continuous stroke Press the foot switch once to start sectioning If the foot switch is held down for more than half a secon...

Page 26: ...guide 2 of the specimen clamp into the mount 3 from the left To clamp turn the clamping lever 1 clockwise to the limit stop 9 2 Orienting the specimen Rotate the handwheel to position the specimen cla...

Page 27: ...r base North South North Southdisplacementenablestheapproachofthe knife holder to the sample as close as possible To release turn the clamping lever 1 on the left sideofthemicrotomebaseplatecounterclo...

Page 28: ...ife holder A reference point is provided on the hand rest Moreover a reference line is provided on the right side of the knife holder base to adjust the angle when the hand rest is removed Torelease t...

Page 29: ...all section waste to the section waste tray Empty the section waste tray Clean the instrument see chapter 13 Cleaning Turn the instrument on with the mains switch Always lock the handwheel prior to m...

Page 30: ...l the other section thickness settings except the 0 5 m setting This is caused by an operating er ror All functions of the instrument are inoperational Do not press any button Turn the instrument off...

Page 31: ...by the technical service 12 1 5 Sectioning motor cannot be restarted The sectioning motor is blocked and cannot be re started The sectioning motor was subject to a short term over load and was cut of...

Page 32: ...ower sectioning speed Select lower sectioning speed Clearance angle adjustment systematically decrease the clearance angle until the optimum adjustment is ob tained Check if all levers are locked and...

Page 33: ...the specimen towards the rear limit 2 Push the coarse feed button at the rear to move the specimen towards the rear limit 1 Mains cable not properly connected 2 Mains fuses defective 1 Loss of the ret...

Page 34: ...panel 1 Press the foot switch shortly 2 Remove foot switch Connect the foot switch adapter Recon nect the foot switch 3 Release both buttons simulta neously if possible 1 Please call the technical se...

Page 35: ...Remove the hand rest for cleaning Remove the knife holder and knife holder base for cleaning Only use mild commercial detergents or soap solution for cleaning Do not use solvents that contain acetone...

Page 36: ...act your local Leica service center Clean the instrument every day Lubricate the following parts with the oil No 405 once a month 1 2 drops will be sufficient T piece 1 on the microtome base plate Gui...

Page 37: ...ent off with the mains switch and pull the mains plug before changing the voltage setting Turn the instrument off with the mains switch Disconnect the mains plug from the mains power outlet Place the...

Page 38: ...mains fuses are located in the voltage selector housing 3 above the mains switch at the rear of the instrument Remove the voltage selector housing 3 as de scribed under 14 2 Remove the fuses 4 Mount...

Page 39: ...and pull it out together with the spring 5a and the pin 5b To remove turn the eccentric bolt 6 counterclock wise Loosen the 2 screws 7 and 2 screws 8 which are accessible through the holes 9 and remov...

Page 40: ...blocks In addition it accommodates the foil clamps Turntheknurledknob 1 counterclockwisetomove the movable jaw 2 downward Mount the sample as required Turn the knurled knob 1 clockwise to move the mov...

Page 41: ...clockwise To orient the sample introduce the pin 9 in one of the holes 10 and turn counterclockwise To fix the orientation introduce the pin 9 again in one of the holes 10 and turn clockwise 15 2 4 Ve...

Page 42: ...hefoilclampinthestandardspecimenclamp as shown Turn the knurled knob 1 clockwise to securely clamp the foil clamp in the standard specimen clamp Clamping of flat samples To clamp flat samples replace...

Page 43: ...nthestandardspecimenclamp so that the bevelled surface 7 on the back of the foil clamp points to the right or left Turn the knurled knob 1 clockwise to securely clamp the foil clamp in the standard sp...

Page 44: ...tlateraldisplacementfunc tion described above Loosen the 2 screws 1 with the provided screw driver and remove the cover plate 2 Insert the backlighting 3 in the recess at the rear of the knife holder...

Page 45: ...knife and dur ing breaks always lock the handwheel and cover the cutting edge with the knife guard Microtome knives and disposable blades Take care when handling microtome knives and disposable blades...

Page 46: ...knife support bar via the height adjust ment screws to the lowest position to prevent dam age to the cutting edge while inserting the knife Unscrew the knife clamping screws 2 counter clockwise as far...

Page 47: ...t bar Inserting the knife Turn the knurled wheels 6 on the right and left of the knife holder in opposite directions to the front to move the knife support bar via the height adjust ment screws to the...

Page 48: ...of the knife holder by using an Allen key size 4 Remove the pressure plate 5 Attach the new pressure plate by tighten the 4 screws 4 Cleaning The clamping plate 7 can be removed for cleaning To remove...

Page 49: ...g lever 2 up Cleaning The clamping plate 7 can be removed for cleaning To remove the blade relocate the clamping lever 2 down Carefully remove the blade Pull out the clamping lever 2 Remove the clampi...

Page 50: ...f the control panel The control panel enables to select the two sectioning modes single continuous stroke and to start and stop motorized sectioning Start motorized sectioning After selecting one of t...

Page 51: ...0402 08320 Holder for EM specimen holder 0502 29968 Segment arc with adapter for EM specimen holder 0502 29969 Specimen clamping system non orienting 0502 29963 Specimen clamping system orienting 0502...

Page 52: ...t and or faulty operation invalidate the warranty and any claims based thereon and likewise Leica will not assume liability for any con sequential damage Disposal The instrument or parts of the instru...

Page 53: ...53 Leica RM 2155 Rotary Microtome Notes...

Page 54: ...54 Instruction Manual V1 1 01 2001 Notes...

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