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Leica asp6025 Instructions For Use Manual Download Page 26

26

Instructions for Use V 2.4 RevL - 12/2015  

3.4.2  Instrument rear panel - terminals

Fig. 3

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38

28 29

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36

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3. 

Instrument Components and Specifications

5 -  Monitor
26  -  Handle for moving the instrument
27  -  USB port (downloading/saving)
28  -  Network connection (LAN)
29  -  USB port 
30  -  Terminal for keyboard (K)

5

31

Connecting a keyboard/mouse is reserved SOLELY for trained Leica employees. This also applies to the network 

connection, which is to be used only together with RemoteCare (service diagnostics).

31  -  Terminal for mouse (M)
32  -  Local alarm connection
33  -  Remote alarm connection
36  -  Power supply input
37  -  Main power switch (ON/OFF)
38  -  Exhaust output

Summary of Contents for asp6025

Page 1: ...SP6025 Advanced Smart Processor Vacuum tissue processor ASP6025Tissue ProcessorV 2 4 English 12 2015 Order No 14 0495 80101 RevL Always keep this manual with the instrument Read carefully before worki...

Page 2: ......

Page 3: ...al details of the present Instruc tions for Use are not to be considered warranted characteristics of our products These are determined only by the contract provisions agreed between ourselves and our...

Page 4: ...and accessories 25 3 4 2 Instrument rear panel terminals 26 3 4 3 Instrument specifications 27 4 Instrument Setup 30 4 1 Unpacking the instrument 30 4 2 Basic instrument hardware 37 4 2 1 Installatio...

Page 5: ...8 5 2 2 The RMS system adjusting warning threshold values 100 5 2 3 The reagent status 104 5 2 4 Configuring the stations 106 5 2 5 The Reagent groups menu 107 5 2 6 Ethanol rotation 108 5 2 7 Adjusti...

Page 6: ...2 6 3 Maintenance overview 164 6 3 1 Daily cleaning and maintenance 166 6 3 2 Periodic cleaning and maintenance 169 6 4 Taking the instrument out of service temporarily 170 7 Troubleshooting 171 7 1 T...

Page 7: ...entalprotectionsymboloftheChinaRoHS directive Thenumberinthesymbolindicatesthe Environment friendlyUsePeriod oftheproduct in years The symbol is used if a substance re strictedinChinaisusedinexcessoft...

Page 8: ...levant standards defined or administered by the American National Standards Institute ANSI Underwriters Laboratories UL the Canadian Standards Association CSA the National Sanitation Founda tion Inter...

Page 9: ...ace tension of the liquid in the interior of the tube This causes the indicator tube to change color Example of labeling in accordance with IPPC IPPC symbol Country code in accordance with ISO 3166 e...

Page 10: ...riant Nameplate for voltage 120V variant Fig 1 Fig 1 is provided as an example only and shows a valid nameplate for this instrument with the necessary infor mation about instrument type and power requ...

Page 11: ...measurement as well as the Ability to replace xylene a substance that is hazardous including to the health with special process protocols The instrument has been designed so that it is safe to use by...

Page 12: ...omply with the following instructions and warnings Thisinstrumenthasbeenbuiltandtestedinaccordancewiththe safety requirements for electrical equipment for measurement control and laboratory use To mai...

Page 13: ...supply voltage other than that to which it was originally set Theinstallationlocationmustbewellventilated andmustcontainnosourcesofignitionofanykind Thechem icals to be used in the ASP6025TissueProces...

Page 14: ...em bottles must be properly pushed home into the connection manifolds at the back panel of the reagent cabinet Failure to plug the system bottles into the manifold correctly will interrupt the infiltr...

Page 15: ...less The exact concentra tion values can only be measured locally The limits were complied with in all operating states Take care when handling solvents and reagents To prevent damage to the instrumen...

Page 16: ...at no part of the body is located in the opening area of the retort lid Since the retort may be under pressure open the bolt handle very carefully and slowly 2 Safety Material safety data sheets can b...

Page 17: ...re is a safety relief valve that vents all excess air pump output to atmosphere Overcurrent protection Overcurrent conditions are protected against by both the main fuse and the separate heating power...

Page 18: ...illinganddrainingofreagents 14 0495 44794 1 Fillinganddraininghoseforremote draining of paraffin 14 0495 46467 7 RTU bottles with cover plastic 14 0495 43542 10 System bottles plastic 14 0495 43329 1...

Page 19: ...4 01005 1 Paraffin station sieve 14 0495 43987 7 Spare covers for RTU bottles 14 0495 44976 1 Paraffin station lid 14 0495 44021 3 Perforated trays for drawer 14 0495 43602 2 Compartment dividers for...

Page 20: ...Storage temperature range 5 C to 50 C Relative humidity 10 to max 80 non condensing IEC 61010 1 classification Protection class 1 Pollution degree 2 Overvoltage installation category II Ambient press...

Page 21: ...g reagents 50 C to 67 C 4 K 0 K General System bottles 9 in reagent cabinet RTU bottles 6 in drawer Condensate bottle 1 Cleaning solution bottles 3 not additional Maximum bottle volume 5 0 l Pretest c...

Page 22: ...h consisting of up to 12 reagent and 3 paraffin processing steps Time per program step 0 to 23 hours 59 minutes Delay time max 6 days Up to 300 cassettes can be processed simultaneously Three cleaning...

Page 23: ...affin approved for histological application Reagents for external cleaning Paraffin remover by Medite 1 HCl ethanol based on 70 ethanol Surface cleaners for plastic Poliboy Paraguard by Polysciences R...

Page 24: ...24 Instructions for UseV 2 4 RevL 12 2015 Fig 2 2 8 1 5 13 15 19 7 4 24 17 14 11 9 6 1 16 22 21 3 10 20 3 18 1 12 12 9 23 23 5 25 18 2 8 2 3 Instrument Components and Specifications 3 4 Overview...

Page 25: ...moving the instrument 24 Cover flap for the active carbon filter 25 Paraffin station lid The specimens are processed in the stainless steel retort 19 at the preselected pressure vacuum and temperatur...

Page 26: ...ownloading saving 28 Network connection LAN 29 USB port 30 Terminal for keyboard K 5 31 Connecting a keyboard mouse is reserved SOLELY for trained Leica employees This also applies to the network conn...

Page 27: ...gurable with up to 15 steps temperature time reagent three pressure vacuum options The quick start system enables you to start each infiltration program immediately from the FAVORITES window max 10 Th...

Page 28: ...scanbeexchangedviatheinfiltrationchamberusingaprocesscontrolled by the instrument for filling and draining from system and RTU bottles i e with a connected reagent hose Five liters of melted paraffin...

Page 29: ...er which has a maximum capacity of 4 8 liters Optionally the ASP6025 can also be operated with reagent volumes of 3 8 liters or 5 liters In the first mode the operator can choose between one and two b...

Page 30: ...must be at least 1 m on the side and rear On the front the distance must be at least 3 m as the ASP6025 is rolled from the pallet in this direction The room height must be at least 2 5 m as the packag...

Page 31: ...e removed from the pallet 87 by pulling it upwards Unpacking and removing the accessories The carton 89 contains the accessories that are not yet installed in the instrument Carefully set the carton a...

Page 32: ...and right with the panel 92 into the groove 93 of the pallet so that they are flush with the wooden board 94 that holds the instrument Fig 9 95 95 Fig 10 Fig 9 Whiledoingso makesurethattheplate 92 isb...

Page 33: ...d when rolled off the pallet via the ramp Whentheinstrumentisset up in its final location the brakes on the instrument castors must be engaged once again To do so push the lever 97 detailinFig 10 back...

Page 34: ...8 First takeoutthetwofoamparts 107 nexttothebracket Fig 12 Then you remove the dust cover Before the monitor is screwed onto the bracket you should check that the three terminals 102 power supply USB...

Page 35: ...n key 104 supplied Fig 16 Finally remove the molded foam cushion 98 by lifting it off the monitor 101 103 Unpacking the instrument continued Installing the monitor Todoso liftthemonitoroutofthefoamcus...

Page 36: ...ripsofadhesivetape 105 that secure the doors and the drawer of the instrument 105 Fig 17 9 Fig 18 In the retort you also have to remove the strip of adhesive tape 105 that secures the cover of the lev...

Page 37: ...Avoid vibrations direct sunlight and heavy variation in temperature 4 2 1 Installation site requirements CAUTION ThechemicalstobeusedintheASP6025areeasilyinflammableandhazardoustohealth Therefore the...

Page 38: ...evice Even if the instrument is connected to an external exhaust device the active carbon filter provided must remain in use Theflangekit Fig 21 consistsoftheexhausthose 74 50 mm and the exhaust flang...

Page 39: ...n key 76 atatorqueof0 5Nm Figs 22 23 Makesure thatthefanplateandtheflangeareflushagainst each other Installing the tube for external exhaust air continued Fig 23 Fig 24 Nowattachoneendoftheexhausthose...

Page 40: ...her than that to which it was originally set The power supply voltage for the instrument is factory preset and CANNOT be altered by the user Caution Observe the following instructions carefully to pre...

Page 41: ...luid level in the retort is lowered Then the retort is released and can be opened Pressing NO continues the process To continue the process after opening the retort move the handle back into the locki...

Page 42: ...t is loaded with the fill volume can be selected in the dialog box by pressing the corresponding button Fig 31 For this purpose there are three level sensors 40 1 40 3 Fig 31 located behind the cover...

Page 43: ...ecarbonfilter 13 opentheflap 24 behind the retort lid 24 13 1 13 Push the filter in as far as it will go with the handle facing forwardsinthedirectionofthe arrow as shown in Fig 32 Thedateonwhichthefi...

Page 44: ...spiral the cassettes can be packed in more tightly so that a maximum of 100 cassettes can fit in the basket Eachmetalbaskethasamovable canbeswungtotheside handle 49 for inserting and removing it from...

Page 45: ...gents The touchscreen itself is resistant to all reagents used in the instrument Despite this avoid contact between the two Immediately wipe up any reagent splashes USB port Rear side of the monitor w...

Page 46: ...Fig 38 The level must not fall below these marks 56 55 4 Instrument Setup Fig 37 56 Enlarged detail Sieve in the Paraffin station 54 Fig 38 Top mark Minimum fill level when filling with pellets for m...

Page 47: ...can also be removed from the compartment for cleaning Fig 41 11 57 58 15 58 58 Thebathscanbefilledwithliquidparaffinmanuallyorfrom theparaffinstation Itisalsopossibletofillwithpellets the melting time...

Page 48: ...lparaffinbathshavetwohandles 59 forpullingoutand carrying Two movable covers 60 1 and 60 2 provide for better thermal insulation and prevent the liquid paraffin from sloshing out when being pulled out...

Page 49: ...rtically out of the RTU bottle while holding down the tapered pump head against the bottle neck Remove the unwanted RTU bottle and replace with a new one in the drawer Detail Fig 44a 61 62 63 63 Fig 4...

Page 50: ...cleaning program by detergent Each container has a screw plug Each container has a max capacity of 5 l Fig 47a detail Only RTU bottles from Leica are approved for this instrument However if different...

Page 51: ...the SMART SCREEN in a separate area on the screen They are designated D1 to D6 Fig 48 Additionally the RTU bottles can be emptied using the remote drain system ThehosefortheRemoteFill Drainsystemcanal...

Page 52: ...serting a system bottle always ensure that the retaining ring 35 is tightened and the connection port 65 clicks into place correctly with the O ring 65 1 65 1 65 Regularly grease the O ring 65 1 withM...

Page 53: ...it if necessary Reagent cabinet with system bottles continued 4 4 9 The drip tray 16 Fig 53 Condensate bottle Thecondensatebottle 14 Fig 52 hastheexactsameshape and connection design as the system bo...

Page 54: ...olor palette for the individual reagent groups in the instrument The labels 72 are applied to the front of the system bottles 15 as shown in Fig 55 The surface on the system bottles must be clean and...

Page 55: ...to bottom The symbol at 5 l Detail 2 in Fig 57 means that the RTU bottle must be filled up to that point if the retort is to be filled up to the third level sensor corresponds to loading with three b...

Page 56: ...any keys Plug the power cable 39 Fig 26 into the POWER IN socket on the rear side of the instrument Then connect the cable to the power socket If applicable switch on the switch for the power socket T...

Page 57: ...UN LOG 3 4 2 1 5 8 7 6 The SYSTEM DIAGNOSTICS menu The following functions can be selected by touching the corresponding key 6 Abortsthecurrentprogramanddeletesthecurrentallocation of reagents to bott...

Page 58: ...mentalarmisconfirmedwiththe YESbutton thealarmsoundisswitchedoffandthemessage disappears from the screen No other alarm takes place no local alarm and no remote alarm The alarm sound can also be disab...

Page 59: ...red when the instrument cannot complete an infiltration program If in such a case all alarm notifications have remained unanswered by pressing the YES button the remote alarm is triggered Like the loc...

Page 60: ...ed via a 3 pole stereo jack Thealarmpinoftheconnectoristhecenterterminal 68 onthe stereo jack Depending on the configuration of the polarity the innerortheouterterminalofthestereojackisswitchedthrough...

Page 61: ...in the bottom bar supervisor mode has been disabled A user is logged in The program is currently running the symbol shows a wheel that is turning 34 Status Bar Thebluebaratthetopofthescreeniscalledthe...

Page 62: ...at is disabled in many cases a dialog window appears explaining the reason why the function is currently not possible Button symbols Press the corresponding button symbol to enable functions on the to...

Page 63: ...ers and back AltGr Permits the entry of special characters Deletes the previous character Delete Deletes the entire line OK Takes over the entered term 1 Functions of the touchscreen continued In the...

Page 64: ...ing it on the touchscreen Fig 68 Activated symbols are surrounded by a red border Touch the symbol a second time to disable the station The stations system bottles and RTU bottles are displayed in the...

Page 65: ...witch on the rear panel of the instrument Item 37 in Fig 3 Fig 26 4 Instrument Setup Fig 70 Pressing the NO button leads back to the original program window 37 Caution The ASP6025 is allowed to be swi...

Page 66: ...displays the parameters for the ASP6025 which are to be configured during installation Fig 71 5 1 1 The Installation menu The serial number of the instrument and the current software version are ente...

Page 67: ...onding row Press OK and the selected language is set 5 Operation Fig 73 Fig 74 TouchtheINSTRUMENTNAMEbuttonandenteraninstru mentname whichmaynotexceed20characters usingthe keyboard The instrument name...

Page 68: ...ow the instructions on the screen Calibrating the touchscreen Fig 78 Fig 78 2 The software update is delivered on a USB stick Connect the USB stick and press the UPGRADE SOFTWARE button Fig 77 Then fo...

Page 69: ...ght areas Program options Basket mode Measure concentration Date Time Security Alarm relay Instrument Acoustic instrument alarm 5 1 2 System settings Fig 79 Toswitchtothesystemsettings selecttheSYSTEM...

Page 70: ...o switch to another mode An in struction message appears Fig 81 which lists the steps the user is required to carry out so that the instrument will operate without errors in the new mode This message...

Page 71: ...me DISABLED mode The number of cassettes inserted into the retort is not queried prior to the program start If values for a reagent that is used in a pro gram are entered in the CASSETTES UNTIL CHANGE...

Page 72: ...utton for the setting desired in the MEASURE CONC field Fig 85 86 Agreencheckappearsfortheselected option 5 Operation Fig 85 Fig 86 Fig 87 When alcohol concentration mea surement is enabled it takes p...

Page 73: ...press OK to confirm System settings continued INSTRUMENT Fig 90 Here you can select the maximum number of baskets with which the retort can be loaded Dependingonthisselection theretortisfilledwithther...

Page 74: ...E andTIME Setting the date PressingtheDATEbuttonopensthesettings window Fig 92 Use the MOVE UP and MOVE DOWN but tons to make the necessary settings and adjustments 5 Operation Fig 92 Setting the time...

Page 75: ...selected a program report is generated after the infiltration is complete and can be saved as a PDF file The report contains the following data on the program that has run The program name The program...

Page 76: ...isplayed beside the button Fig 95 ACOUSTIC INSTRUMENT ALARM Fig 96 System settings continued Fig 95 Touchingtherespectivebuttonopensaselectionmenu Fig 96 in which you can select the sound to be heard...

Page 77: ...TY INV each touch of the button toggles between OPENING CONTACT and CLOSING CONTACT Configure the buttons such that there is NO alarm sounding SettheassociatedTESTbuttonto ON Thealarmshouldnowsoundaga...

Page 78: ...er of the screen A password entry is not necessary to switch to operator mode Operators can run the following functions Start programs enter the number of cassettes and change the run options Interrup...

Page 79: ...nstrument cannot be used Lock mode is disabled again by entering a password In order to enable lock mode proceed as follows In the Security field press LOCK MODE The keyboard appears Use it to choose...

Page 80: ...ningservesasaremindertoschedulemaintenanceviatheLeicaTechnicalService Youcancontinue using the instrument without any limitations If the set alarm threshold value is exceeded a message is output on th...

Page 81: ...or mode neither of these values can be changed If the stored warning value is exceeded a warning message regarding the age of the carbon filter is output Thiswarningservesasaremindertoreplacethecarbon...

Page 82: ...lted as determined by the instrument can be corrected by selecting the PARAFFIN BATH MELTING TIME button Thisfunctionshouldnormallyonlybeusedtoincreasethemeltingtime e g if paraffin pellets have been...

Page 83: ...e appears which must be confirmed Then the current reagent list is displayed and it is thus possible to change the reagent status of the retort by selecting the correct reagent Reagent status of the r...

Page 84: ...ding new symbol is displayed Symbol System status OK Warning Error Error status SeriouserrorsoftheASP6025havebeendetectedduringoperation Thedetectederrorisdisplayed on the screen in a message It must...

Page 85: ...ample on the left Fig 111 This message must be confirmed with OK Ifanerroroccurs itwillremainpresentinthesystemstatus until the problem has been corrected Fig 111 Fig 112 Depending onthenatureofthepro...

Page 86: ...he system status always reflects the most serious error in the list Fig 113 The list in the SYSTEM STATUS window has the following columns Severity The severity indicates one of the two levels Warning...

Page 87: ...ded in the run log during the entire application The entries are sorted by date the most recent entry being shown at the top The following events are included in the RUN LOG Start and end of each prog...

Page 88: ...ith the highest priority level Events with the medium priority level Level 1 The following are displayed Only events with the highest priority level The Run log continued A priority level is assigned...

Page 89: ...ns Fig 115 After it is opened all entries that have been recorded by the ETERNAL ERROR LOG throughout the entire application can be viewed The entries are sorted by number the most recent entry is sho...

Page 90: ...ress the ENTRIES and SAVE TO USB buttons to open the window of the same name InsertaUSBstickinoneoftheUSBportsontheinstrument and press the COPY FILES button Fig 116 Adisplayonthescreenshowswhichdatai...

Page 91: ...B stick back to the instru ment proceed as follows InsertaUSBstickinoneoftheUSBportsontheinstrument and press the LOAD button Press the ENTRIES and LOAD FROM USB buttons to open the window of the same...

Page 92: ...s the COPY FILES button TheinstrumentsoftwarewillcheckwhetherallPDFprintouts storedontheinstrumentarealsopresentonthestick Ifthis is true the backup is not performed and a message to this effect is ou...

Page 93: ...iruses Then insert the USB stick according to Chap 4 4 4 into the provided socket on the instrument 3 Click INCIDENT REPORT 4 In the DATE OF INCIDENT section of the opened dialog select either the day...

Page 94: ...from a previous save is on the USB stick this data is overwritten when another save is carried out If this happens a corresponding message appears Click YES to confirm the overwrite If you want to ch...

Page 95: ...the retort is displayed beside the retortandthetargettemperatureoftheparaffinappearsbelow the paraffin baths System bottles 15 are designated S1 S9 RTU bottles 10 are designated D1 D6 The reagent cont...

Page 96: ...Screen To do this press the SET TEMPERATURE button and call up the SELECT THE RETORT TEMPERATURE entry window Fig 132 The temperature of the retort can be entered here Thepossibletemperaturerangeisfro...

Page 97: ...h solid paraffin press the PARAFFIN MELTING button A message will appear indicating the current melting time Fig 135 The paraffin station will remain locked while the melting process is taking place F...

Page 98: ...NINGTHRESHOLDS window opens Fig 136 To add a reagent proceed as follows Press INSERT to display the keyboard Enter the new reagent name Press OK to confirm You will automatically be prompted to select...

Page 99: ...ingentryscreenappears Fig 138 Enteranewthresholdvalueor ifnowarningisdesired press CLEARtoremovethethreshold altogether Press OK to confirm Changeable parameters Fig 138 Fig 139 Configuring the reagen...

Page 100: ...ue of this type can be defined depending on the use The RMS has two areas The warning threshold values which are entered for all reagents in the reagent list and displayed in the reagent status The re...

Page 101: ...is also displayed at the end of a program after the cleaning Fig 143 Fromthere youcanpressYEStogodirectlytotheREAGENTSTATUSwindow Fig 141 Fig 142 Fig 143 Fig 144 A warning message when starting a pro...

Page 102: ...used according to the preinstalled protocols by entering them into the SET UP REAGENTS WARNINGVALUES menu 5 Operation These warning threshold values are set to 55 at the factory However the user can...

Page 103: ...lene after 6 cycles Cleaning alcohol after 6 cycles Cleaning water after 6 cycles 1 for a low throughput of approx 100 cassettes daily Xylene free protocols Reagent recommended replacement Formalin af...

Page 104: ...s of each station full or empty Criteria that trigger warning messages Fields colored in red Tochangethestatusofareagent highlightthe correspondingrowandpressthebuttonforthe respective action The crit...

Page 105: ...ltherespective system bottle from an external station or fill the system bottle manually Afterfillingabottlemanuallythebottlemustbedefinedasfull Adding new reagents to stations To do this open the REA...

Page 106: ...nt and confirm your selection with OK The highlighted reagent is assigned to the station The VIEW EDIT STATIONS window Fig 147 shows a list of all stations with the assigned reagents that have been de...

Page 107: ...le header the SELECT COLOR window appears Fig 148 Select the color you want and confirm with OK Thenewlyselectedcolorwillnowbeusedonallstationstorepresentthehighlightedreagentgroup Thecolorwillapply f...

Page 108: ...ram checks this when it is started If there is a discrepancy a warning message appears and the program cannot be started In CONCENTRATION mode the system bottles that are filled with ethanol in row S1...

Page 109: ...oned into the S1 system bottle from S3 into S2 etc IfNO Fig 150 ispressed thesubsequentprogramstartsWITH OUT alcohol rotation 1st step Theethanolvalueforreplacementisdeterminedwithethanolfromsystembot...

Page 110: ...rmed with YES before the program is started The ethanol is then transferred to system bottle S6 while the program is running Functional description of ethanol rotation continued If NO Fig 152 is press...

Page 111: ...Fig 156 appears From the start screen press ENHANCE MENT on the right hand side to go to the window of the same name 5 2 7 Adjusting the threshold value for ethanol rotation Here you can configure the...

Page 112: ...YESsothatthereplacementprocessdescribed below can be carried out 5 2 8 Replacing xylene Then the exhausted xylene will be transferred to RTU bottle D3 during the program sequence The remaining reagent...

Page 113: ...ist of all freely editable programs currently defined in the ASP6025 Program duration cannot be specified It is determined by the total duration of all program steps plus the estimated fill and drain...

Page 114: ...nter the new program name Editing program steps Pressing the EDIT button in Fig 158 calls up the Program steps screen Fig 160 In the headline you will find the PROGRAM NAME The colors on the left bord...

Page 115: ...Tissue Processor Editing program steps continued Toeditastep highlightthecorrespondinglineandpresstherespectiveheadline In the entry windows that pop up enter select the program step settings Fig 160...

Page 116: ...the highlighted step If required modify properties of the step UsetheMOVE UP MOVE DOWNbuttonstomoveprogramstepsupordownwithinanexist ing program without having to recreate those steps Deleting program...

Page 117: ...dling reagents Always wear rubber gloves and safety goggles when handling the reagents used in this in strument Reagents used for tissue infiltration can be both toxic and or flammable When filling or...

Page 118: ...screen for manual operation of the instrument 79 79 70 Important Dispose of waste solvents with care according to local regulations and the waste manage ment policy of the company or institution ATTE...

Page 119: ...encompleted Fig 167 Tocontinueworking confirmthis with OK If the same reagent will not be used in the next operating step the retort may have to be cleaned with a cleaning program Apromptappearsforcar...

Page 120: ...be selected Fig 169 Afteryouhaveselectedareagent ascreenmessageappears prompting you to make the necessary preparations When everything is finished press YES to start filling Fig 170 5 Operation On t...

Page 121: ...en the selected station is filled with reagent a message windowappears Fig 172 withamessagetotheeffectthat thehoseforremotefilling drainingisbeingblownoutwith air Therefore leave the hose in the remot...

Page 122: ...etainingring 35 issecurelytightenedandtheconnection spout 65 clicks into the back panel correctly Then intheSETTINGSwindowpresstheREAGENTSTATUS button and call up the table of the same name Fig 175 Se...

Page 123: ...en the selected paraffin bath is filled from the retort Finally the retort has to be cleaned Fig 177 Fig 178 The paraffin station Fig 176 If a paraffin bath is filled with pellets the melting time wil...

Page 124: ...lled with paraffin pellets Filling a paraffin bath continued Todoso gotothereagentstatus selecttheparaffinstation and press the SET AS FULL button 5 Operation When the paraffin station is closed a mes...

Page 125: ...e the hose from the external re ceptacle until this cleaning step is complete 57 Using the SMART SCREEN button call up the SMART FUNCTIONS screen Fig 181 Fig 180 5 4 3 Draining a paraffin bath Connect...

Page 126: ...the retort When the bath is emptied the paraffin is automatically pumped from the retort into the external container After this operation is complete the paraffin residues are blownoutoftheexternaltub...

Page 127: ...gram press the START button then select the level number of baskets in the retort The user has the option of changing the program he has called before starting it Steps can be deleted or added or para...

Page 128: ...e or they prompt for entry of a desired end time when the program starts Starting a Favorite program TobeginworkingwithaFavoriteprogram press the corresponding symbol in the FAVORITES window After sta...

Page 129: ...nd press OK to confirm The name of the program is transferred to the empty line Before a program appears with a Start button in the FAVORITES window it must be defined as a Favorite program To do so p...

Page 130: ...f a desired end time until the program is started Press OK to confirm the ending time Defining Favorite programs continued Fig 190 Assigning a day for the end time If an end time has been assigned to...

Page 131: ...ogram you want to delete in the VIEW EDIT FAVORITES window Touchthe DELETEbutton Thehighlightedprogramwillbedeletedwithoutasecurityprompt 5 Operation The first position in the list is empty This optio...

Page 132: ...number of cassettes to be processed must be entered Theinstrumentcontrolcheckstheremainingmeltingtimefortheparaffinbathswhentheprogramstarts Iftheruntime is expected to be extended the operator must...

Page 133: ...ll prompts have been confirmed If the program was called up from the PROGRAMS window the START button must be pressed to start the program Only completely cleaned cassette baskets may be used for tiss...

Page 134: ...assettes that are being pro cessed in the retort is displayed The work step currently running and the time until it ends are displayed in the field below that You can switch from the graphical to the...

Page 135: ...PDF file Fig 197 If you start a pro gram for which TIME REQUEST was selected as the end time an entry window Fig 197 opens in which you can select the end time This function Fig 198 shows the options...

Page 136: ...the paraffin an automatic dripping time of 2 minutes is implemented in order to optimize the use of cleaning reagents Once an infiltration program has ended the retort can be drained basket by basket...

Page 137: ...rotation All of the permanently installed infiltration protocols described in this chapter have to be validated before use i e tissue processing using patient tissue for diagnostics by the laboratory...

Page 138: ...Instructions for UseV 2 4 RevL 12 2015 5 Operation Standard tissue over night All specimen types that can fit into a standard cassette tissue with a high fat content may require a longer protocol Fig...

Page 139: ...025Tissue Processor 5 Operation Standard biopsy Allbiopsiesupto3mmdiameter Gastrointestinalbiopsies tissuecoresfromkidneys prostate liverandchest punchbiopsies from skin tissue small intestinal polyps...

Page 140: ...140 Instructions for UseV 2 4 RevL 12 2015 5 Operation Small Specimen All kind of small specimen to maximum dimensions of approx 10 x 15 x 2 5 mm w x d x h Fig 202...

Page 141: ...ol 100 S6 Ethanol 100 S7 Xylene S8 Xylene S9 Xylene D1 Formalin D2 Ethanol 90 D3 empty D4 empty D5 Cleaning xylene D6 Cleaning alcohol Pre installed xylene infiltration programs cannot be renamed edit...

Page 142: ...142 Instructions for UseV 2 4 RevL 12 2015 5 Operation Xylene protocol 1 h Endoscopic biopsies and needle biopsies from chest and prostate Diameter up to 1 5 mm Fig 203...

Page 143: ...5Tissue Processor 5 Operation Xylene protocol 2 h Allbiopsiesupto3mmdiameter Gastrointestinalbiopsies tissuecoresfromkidneys prostate liverandchest punchbiopsies from skin tissue small intestinal poly...

Page 144: ...for UseV 2 4 RevL 12 2015 5 Operation Xylene protocol 4 h Small specimens from non dense tissue intestine kidneys liver etc with a thickness of up to 3 mm excision and incision biopsies of the skin e...

Page 145: ...145 ASP6025Tissue Processor 5 Operation Xylene protocol 6 h All routine tissues with maximum dimensions of 15 x 10 x 4 mm except specimens of brain tissue and pure adipose tissue Fig 206...

Page 146: ...146 Instructions for UseV 2 4 RevL 12 2015 5 Operation Xylene protocol 12 h Allroutinetissueswithmaximumdimensionsof20x10x5mm tissuewithahighfatcontentmayrequirealongerprotocol Fig 207...

Page 147: ...minutes Bottle Reagent S1 70 ethanol S2 85 ethanol S3 empty S4 80 20 ethanol isopropanol S5 80 20 ethanol isopropanol S6 100 isopropanol S7 100 isopropanol S8 100 isopropanol S9 empty D1 Formalin D2 e...

Page 148: ...148 Instructions for UseV 2 4 RevL 12 2015 5 Operation Xylene free 1 h Endoscopic biopsies and needle biopsies from chest and prostate Diameter up to 1 5 mm Fig 208...

Page 149: ...025Tissue Processor 5 Operation Xylene free 2 h Allbiopsiesupto3mmdiameter Gastrointestinalbiopsies tissuecoresfromkidneys prostate liverandchest punchbiopsies from skin tissue small intestinal polyps...

Page 150: ...s for UseV 2 4 RevL 12 2015 5 Operation Xylene free 4 h Small specimens from low density tissue intestine kidneys liver etc with a thickness of up to 3 mm excision and incision biopsies of the skin el...

Page 151: ...151 ASP6025Tissue Processor 5 Operation Xylene free 6 h All routine tissues with maximum dimensions of 15 x 10 x 4 mm except specimens of brain tissue and pure adipose tissue Fig 211...

Page 152: ...152 Instructions for UseV 2 4 RevL 12 2015 5 Operation Xylene free 12 h Allroutinetissueswithmaximumdimensionsof20x10x5mm tissuewithahighfatcontentmayrequirealongerprotocol Fig 212...

Page 153: ...ning program touchtheCLEANINGPROGRAMSbutton in the RAPID PROTOCOLS field to call up the corresponding window 2 Fig 213 Fig 214 In the CLEANING PROGRAMS menu select the desired program and press VIEW I...

Page 154: ...indow will appear and you can select the desired cleaning program there Youcanselectthefollowingretortcleaningprograms Fig 217 Quick cleaning Regular cleaning Extended cleaning program by detergent wa...

Page 155: ...ycle MUST be carried out this function cannot be canceled The water step can only be selected if cleaning water is present in one of the bottles In Auto by RMS mode Cleaning water must be defined for...

Page 156: ...cessary conditions have been satisfied press the YES button to start the cleaning program The procedure is displayed graphically on the SMART SCREEN Fig 221 Fig 223 If threshold values in the reagent...

Page 157: ...y step 5 Cleaning water 1 65 Ambient II Standard cleaning program Step Reagent Time min Temp C Pressure Vacuum 1 Cleaning xylene 12 67 Ambient 2 Cleaning alcohol 6 62 Ambient Drystep Empty retort 3 Va...

Page 158: ...ng is that the retort has been thor oughly cleaned using the extended cleaning mode and that there is no reagent in the retort Only in this way is it possible to ensure that no residue from other reag...

Page 159: ...ning continued Step 1 Inthefirstphase thefluidlinesareallpurgedinsequenceusing a blast of compressed air Forthispurpose allsystemandRTU bottles inthereagentcab inet and in the drawer have to be in the...

Page 160: ...arried out of whether a greater than negligible quantity of reagent has ingressed into the retort and whether the vacuum is released quickly enough This serves to detect whether the bottle has really...

Page 161: ...ottle with ethanol 100 and place it in any position in the drawer 2 Mark this RTU bottle as full 3 ThenswitchtotheSMARTSCREENandfilltheretorttothe 2ndlevelfromthisbottle thentransferthereagentbackto t...

Page 162: ...the drip tray Fig 230 16 The collecting tray 16 must be inspected regularly for signs of leaking reagents Fig 230 It is recommended to do this aftertheinfiltrationandcleaningprogramhasbeencompleted b...

Page 163: ...TORmenu tosetthefilterreplacementdatetothecurrentdate Seealso Chapter 5 1 4 Fig 233 Emptying the condensate bottle Thebluecondensatebottlemustbetakenout emptiedand reinsertedatregularintervals weekly...

Page 164: ...he seal and clean the retort lid and seal carefully and only with the paraffin scraper provided to avoid damaging the seal and or the PTFE coating on the retort lid X Clean the retort X Clean the leve...

Page 165: ...y screwed into place X Clean the outer surface of the ASP6025 X Check the filter in the paraffin station and clean it if necessary X Check and clean the lids of the paraffin baths X Clean the paraffin...

Page 166: ...seals immediately 1 Pull out the old seal from the groove 2 Ifnecessary cleanthegroovewithaparaffinscraperandapermittedreagentforexternalcleaning referto Chapter3 3 3 Press the new seal into the groo...

Page 167: ...creen Call up CLEAN TOUCHSCREEN under PROGRAM PROTO COLS on the SETTINGS MENU screen Clean the touchscreen Press ENABLE EXIT to enable the BACK button Fig 236 This will enable you to return to the sta...

Page 168: ...Remove the screen 40 Fig 238 in front of the sensors Cleaning the level sensors Use the cleaning tool together with the microfiber cloth as shown in Fig 239 and 240 Fig 238 Fig 240 Fig 239 40 The lev...

Page 169: ...ng lubricant supplied Connection port with delivery hose 65 1 Fig 242 Connection port Condensate bottle System bottle Fig 241 65 35 35 65 1 65 The level can be identified by simply looking through the...

Page 170: ...cor rectly plug reagent containers into the manifold will cause an interruption to the processing run and may result in spilling of reagents The level can be identified by simply looking through the...

Page 171: ...in use at the time 7 1 1 Power failures If there is evidence of power to the instrument Verify that the power plug is plugged into the socket and that the socket is switched ON Verify that the power s...

Page 172: ...y will begin supplying power to the instrument This is indicated by the green LED 107 in the unlocking button 44 illuminated under the retort Fig 244 The specimen protection system is initiated Once t...

Page 173: ...anbekeptin theeventofpowerfailure thoughtheretortmayhavetobe filled to a high enough level so that no tissue sample is dry Thisoperationisautomaticallycontrolledbytheinstrument and cannot be influence...

Page 174: ...rogram step going backwards until a safe reagent program step It is also possible to proceed to the next step if the current step was completed and a safe reagent is present in the next program step A...

Page 175: ...automatically and leads to a safe specimen state being initiated Example a reagent station is underfilled and no alternative reagent is available Likewise defective instrument components such as valv...

Page 176: ...deposits have collected under the retort seal 3 There is a blockage in the air or reagent lines Blockages in the reagent lines are typically caused by paraffin or tissue debris If it is not pos sible...

Page 177: ...part of the body is located in the opening area of the retort lid Since the retort may be under pressure open the bolt handle very carefully and slowly 7 4 1 Electrical standard emergency unlock To tr...

Page 178: ...1 Puncture the seal Fig 248 2 with the Allen key Fig 248 1 included in standard scope of delivery and push the slider behind the seal downward Manual unlocking of the retort is triggered 2 Graspthebol...

Page 179: ...m bottles 14 0476 39720 O rings 9 5x2 5 FKM 9 ct 14 0253 45880 Spare covers for RTU bottles 14 0495 44976 Cover seal normal 10 ct 14 0461 36136 Stirrer 14 0495 46070 Set of adhesive labels for system...

Page 180: ...rrantyterms ofyourLeicasalesorganizationortheorganizationfromwhichyouhavepurchasedthecontractual product shall apply exclusively Service information Ifyouareinneedoftechnicalcustomersupportorsparepart...

Page 181: ...e carried out on the ASP6025 vacuum tissue processor based on the instructions for use with version number by a by more than one trained Leica employee s product specialist s Leica employee s product...

Page 182: ...ticles sent to Leica without proper decontamination documentation If you have any further questions please call your local Leica organization Leica Internal Use If applicable note corresponding Job an...

Page 183: ...never possible please use the original transportation case box Yes No 3 2 Signature Date Name Position eMail Institute Department Address Phone Fax Important to avoid refusal of shipment Place one cop...

Page 184: ...184 Instructions for UseV 2 4 RevL 12 2015 12 Notes...

Page 185: ......

Page 186: ...www LeicaBiosystems com Leica Biosystems Nussloch GmbH Heidelberger Strasse 17 19 D 69226 Nussloch Germany Phone 49 6224 143 0 Fax 49 6224 143 268 Internet http www LeicaBiosystems com...

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