AZURA® Detector RID 2.1L / RID 2.1L HighFlow Instructions V6750
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HPLC glossary
Term
Definition
Degasser
Degasser module for fluids
Detector
device measuring the composition or the quantity of a substance.
GLP
Good laboratory practice Good Laboratory Practice)
Gradient
Time-dependent composition of solvent (mobile phase) on low-pres-
sure or high-pressure side of system
Isocratic
Mode of sample separation where the composition of a solvent
remains constant
Calibration
A process for correcting measuring values by the value that a measur-
ing device deviates from the standard.
Capillary
thin metal or PEEK pipe that connects components and devices within
the chromatography system
Correction factor
Factor that arithmetically corrects device-related deviations from mea-
suring values.
Solvent
mobile solvent transporting substances to be separated or isolated
through the column
Luer-Lock
standardized connection system between syringes and cannulaes
Mobile phase
mobile solvent transporting substances to be separated or isolated
through the column
Packaging material
The adsorbent, gel, or solid support used in the chromatography
column.
Preparative
Isolating the maximum amount of a substance in a short amount of
time in a required purity.
Sample
Mixture of different components, which are to be separated using chro-
matography. They are transported by the mobile phase and dissolved
from the column.
Sample loop
A loop, which is separated from the system by the valve, that contains
sample first. After switching the valve, the eluent flows through the
loop and is flushed to the column.
Retention time
The time required from an injection of a substance until the maximum
concentration of a substance becomes visible.
Backflushing
Backflushing of columns or precolumns to separate heavily retarding
substances by changing the flow direction.
Column
Pipe with final closures, which allow the mobile phase to pass. The pipe
contains the packing materials.
Stationary phase
The immobile phase involved in the chromatographic process. It is the
liquid or the liquid film on the surface of the packing material.
Dead volume
Volume of capillaries and system components between mixing cham-
ber, injector and column as well as between column and detector.
Carrier material
Refers to the support that is used to attach the active ligand, usually by
a covalent bond.
