AZURA® Detector RID 2.1L / RID 2.1L HighFlow Instructions V6750
Product information 39
Regularly check if all fittings are tight (5 Nm for stainless steel fittings,
0.5 Nm for PEEK fittings).
All smooth surfaces of the device can be cleaned with a mild, commer-
cially available cleaning solution, or with isopropanol.
Device defect
Intruding liquids can cause damage to the device.
Place solvent bottles next to the device or in a solvent tray.
Moisten the cleaning cloth only slightly.
10.2 Check calibration/validation
Calibration solution has been prepared: 350 mg sucrose is weighed in a
100 ml volumetric flask and filled to the mark with deionized water.
calibration solution
deionized water
syringe
Procedure
1.
Open the flush valve.
2.
Flush the detector for 5 min with deionized water at 1 ml/min.
3.
Close the flush valve.
4.
Continue to flush at the same flow rate until the baseline drift is
below 1 µRIU/h.
5.
Push autozero.
6.
Remove the capillary from the IN port.
7.
Fill the syringe with the prepared calibration solution.
8.
Install the syringe on the IN port.
9.
Slowly and carefully inject the sucrose solution into the detector IN
port.
10.
Wait until the baseline is stable.
When the baseline is stable, the measured value is read out. This value
should be 512 µRIU ± 5 %.
Repeat the process in case the result is not achieved.
10.3 Preparing the detector for storage or
transport
Pay attention that all hoses and capillaries have been emptied or filled
with flushing solution (e. g. isopropanol) before storage. To prevent algae
formation, do not use pure water.
We recommend the same ambient conditions (temperature range and
humidity) for storage as during operation (see Page 39 ).
Flow cell has been filled with isopropanol.
Detector has been switched off.
Open-end wrench
Prerequisites
Auxiliary material
Result
Next Steps
Prerequisites
Tools
