31
Calibration
4000 MiD® Instructions, V6695
8.3 Flushing the system after calibrating
After the calibration routine is completed, the calibration kit, SFI and
spraychip should be flushed to remove any trace of the calibration solu-
tion.
1.
Disconnect the SFI plug and infusion line from the SFI.
2.
Manually flush the syringe and the infusion line with 50:50 (v/v) mix-
ture of HPLC grade methanol and water with no additives.
3.
Connect the PEEK tubing to the SFI input and waste line ports.
4.
Attach the SFI to your LC system and infuse 50:50 (v/v) mixture of
HPLC grade methanol and water with no additives at 1 mL/min for
5 minutes.
5.
The system is now clean and ready for use.
9. Maintenance
9.1 General maintenance
There are no user serviceable parts in the 4000 MiD. The maintenance
procedures described in this chapter must be carried out with the MiD in
its installed location.
To perform any of the maintenance procedures the door has to be ope-
ned with the safety interlock engaged. If in any doubt, power down the
instrument (see the section „6.2.3 Shutting down the MiD“ on page 28)
and perform the maintenance with the instrument disconnected from the
mains.
The external surfaces of the 4000 MiD can be cleaned by wiping the sur-
faces down with 10% aqueous isopropyl alcohol.
It is recommended to perform periodic inspections and maintenance of
the 4000 MiD components to ensure its operational performance. The
recommended inspection and maintenance periods are listed in the table
below and presented as guidance only. The frequency of the maintenan-
ce will vary depending on the system usage and type of sample analysed.
It is recommended to flush the system daily with Methanol and Water
for 10 minutes to remove the sample from the microfluidic lines and
minimise system carryover.
Recommended inspection and maintenance periods for the 4000 MiD
components:
Components
Daily
Weekly
Monthly
Page
System flush
X
Spraychip
X
Vac-chip
X
SFI
X
DFI
X
Vac-chip flange
X
Procedure
