53
Appendices
4000 MiD® Instructions, V6695
Note:
It is important to note that actual ratios may differ due to various
factors, such as, liquid composition. Adjustments to tubing lengths may
be required.
Calculate an appropriate split ratio and attach the required length of
PEEK tubing to the waste port of the splitting tee. Attach the nut, finger
tighten. The other end can either be directed to waste or to another ana-
lytical instrument, for example, a UV detector.
Note:
The split ratios can change if the PEEK tubing from the waste port
is attached to another analytical instrument. The theoretical calculations
provided in this manual cannot be used for such systems.
Once established, measure the flow rates of different gradient compo-
sitions to establish any variations in flow. KNAUER recommends that the
flow rate throughout the gradient should be 0.3–2 µL/min.
Appendix B: Auxiliary I/O
The auxiliary I/O port (Aux I/O) is located at the rear of the 4000 MiD (see
Fig. 3 on page 13). The port can be used to supply and receive analog
voltages and also to send/receive triggers. The port is a 15-way D-type
male socket and is shown in more detail in Fig. 49.
Fig. 35
Auxiliary I/O port
The port contains two optically isolated digital inputs and two optically
isolated digital outputs. The digital inputs require 10 mA to activate, this
can be supplied via the 5 V reference also available at the connector (see
table below). The 5 V output is protected by a resettable fuse which trips
at 100 mA. The digital outputs are open collector, and are rated at 50 mA.
There port also houses four optically isolated analog inputs and four opti-
cally isolated analog outputs. The inputs have an impedance of 100K, and
the outputs can source or sink 10 mA. Both the inputs and outputs have
ranges of 0 to +1 V full scale.
Caution
Any external instrument sending/receiving triggers and/
or analog voltages to the 4000 MiD should not exceed the input/
output values stated here. The manufacturer is not responsible for
any damage to external instrumentation from improper connec-
tions made to the 4000 MiD.
Attention
Tout instrument externe envoyant / recevant des dé-
clencheurs et / ou des tensions analogiques du 4000 MiD ne doit
pas dépasser les valeurs d’entrée / sortie indiqués ici. Le fabricant
n’est pas responsable de tout dommage à l’instrumentation ex-
terne de connexions incorrectes sur le 4000 MiD.
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