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Product information
4000 MiD® Instructions, V6695
Note:
The orientation of the spraychip 200 will vary when fit-
ted.
5.
For removing the spraychip from the SFI reverse steps 1-5.
The SFI can be fitted to the MiD in two orientations, shown in Fig. 13.
The functions of the two tubing connections on the main body are
not interchangeable. They are used as the input line and as the waste
line of the split.
Fig. 13
SFI orientations
When creating a split with the SFI, it is important to note the following:
KNAUER recommends a flow rate of 0.3 - 2 µL/min to the
spraychip 200.
The split ratio can be changed by changing both the inner diameter
and the length of the waste line (high flow tube).
The split ratio varies with the input flow rate and liquid composition
(changes during a gradient).
Flow rates can be measured using the flow meter assembly, see sec-
tion „8.4 Liquid flow rate measurement“ on page 35.
For a fixed length decreasing the diameter of the waste line increases
the spraychip flow rate.
For a fixed diameter increasing the length of the waste line increases
the spraychip flow rate.
Changing the diameter of the waste line has a greater effect on the
split ratio than changing the length.
To reduce the delay time between the liquid system and any associated
signal (for example a UV cell) the length of the fluidic connections be-
tween the liquid system and the SFI should be kept to a minimum.
You can create your own split by connecting an appropriate waste line to
the SFI. Appendix A: Flow Splitting details how to calculate the theoretical
flow rates from a ratio of tube lengths and diameters.
Waste line
Input line
Input line
