52
Appendices
4000 MiD® Instructions, V6695
Equation 2
Flow rates in units of µL/min
Equation 2 can be rearranged to give Equation 3.
Equation 3
Equation 1 can be used to estimate the length of the high-flow tube that
is required. For example, if an HPLC method uses a flow of 1.0 mL/min,
using Equation 2 to achieve a flow rate of 1 µL/min in the spraychip 200
(low flow tube).
For the SFI the following is constant.
Using Equation 1.
If the inner diameter of your desired waste tube is 254 µm then,
Note:
It is always better to start with a longer piece of tubing (at least
10 cm longer) so that if the low flow rate is too high, the length of the
waste tube can be cut to provide a suitable flow rate.
SFI split ratios for different waste tubing lengths and diameters
Waste
tubing
length/cm
Split ratio
for tubing ID
0
.
005“ (127 µm)
Split ratio
for tubing ID
0
.
007“ (178 µm)
Split ratio
for tubing ID
0
.
01” (254 µm)
25
167.8
647.6
2685.2
50
83.9
323.8
1342.6
75
55.9
215.9
895.1
100
42.0
161.9
671.3
150
28.0
107.9
447.5
200
21.0
81.0
335.6
250
16.8
64.8
268.5
