Kendro Heraeus Biofuge haemo Instructions For Use Manual Download Page 2

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How to use this manual

Use this manual to get acquainted with your centri-
fuge and its accessories.
The manual helps you to avoid inappropriate han-
dling. Make sure to keep it always close to the centri-
fuge.

A manual that is not kept handy cannot provide
protection against improper handling and thus
against damage to persons and objects.

The manual comprises chapters on

•

Safety regulations

•

Instrument description

•

Rotor program and accessories

•

Transportation and hook-up

•

Use of the centrifuge

•

Maintenance and care

•

Troubleshooting

•

Technical data

•

Index

Please fold out

Overleaf you will find a graphic repre-

sentation of the control panel of the

Biofuge haemo

with a survey of the

most important functions

Summary of Contents for Heraeus Biofuge haemo

Page 1: ...Instructions for use Biofuge haemo ...

Page 2: ...de protection against improper handling and thus against damage to persons and objects The manual comprises chapters on Safety regulations Instrument description Rotor program and accessories Transportation and hook up Use of the centrifuge Maintenance and care Troubleshooting Technical data Index Please fold out Overleaf you will find a graphic repre sentation of the control panel of the Biofuge ...

Page 3: ...Biofuge haemo set set 1 2 0 0 0 1 0 speed time lid quick run start stop INSTRUMENTS ...

Page 4: ...ible if connected with mains supply quick run short term acceleration as long as key is pressed with indication of run time passed start stop normal start or manual stop set speed time selection of the parameter to be changed speed or run time stepwise increase decrease of preset values for the selected parameter speed or run time accelerated change when pressed permanently Error codes troubleshoo...

Page 5: ...for rotor order no 7500 3325 13 Other accessories 13 Before use 15 Where to install the centrifuge 15 Mains connection 15 Removing the transport protection 15 Operation 17 Transport and installation 17 Mains connection 17 Opening the lid 18 Emergency lid release 18 Loading the hematocrit rotor 19 Inserting the hematocrit rotor 19 Important application information for rotor 7500 3325 21 Inserting t...

Page 6: ...uring the hematocrit value 36 Reading harp 36 Reading spiral 36 General hints concerning hematocrit measurements 36 Maintenance and care 38 Maintenance to be performed by the customer 38 Cleaning 38 Disinfection 39 Decontamination 41 Autoclaving 42 The KENDRO service offer 43 Warranty conditions 43 Troubleshooting 44 Problems you can handle yourself 44 In case you must call the Service 46 Technica...

Page 7: ...ept close at hand at all times Proper use The centrifuge is designed to separate liquid suspended materials having different densities and particle size respectively The maximum sample den sity is 1 2 g cm3 at maximum speed Improper use During a run a safety zone of 30 cm around the centri fuge must be maintained where neither persons nor hazardous materials may be stationed The centrifuge may cau...

Page 8: ...is properly mounted Never manually open the lid if the rotor still turns Use only original parts for the centrifuge The only exception are common glass or plastic centrifuge tubes if these are approved for the rotor speed and RCF values of your rotor respectively Never use the centrifuge with the lid open Never use the centrifuge if the paneling has been partially or totally removed Changes in mec...

Page 9: ...22 2 No 1010 1 92 CAN CSA C22 2 No 1010 2 020 94 Safety instructions in this manual This symbol denotes potential hazards to persons This symbol denotes potential damage to the centrifuge or parts in its immediate surround ings General hints are marked with this symbol In addition you are asked to adhere to the pertinent regulations in Germany Regulations for prevention of accidents VBG 4 Regulati...

Page 10: ...For your safety 6 for your notes ...

Page 11: ...lly opened during a run or if lid lock mechanism is out of order If the lid is manually opened during a run or if the lid lock mechanism is out of order a corresponding mes sage appears in the display Lid and OPEN respectively Lid lock mechanism You can open the lid only when the power is turned on and the rotor has practically come to a halt 80 rpm You can start the centrifuge only if the lid is ...

Page 12: ... is dis played above 1 min in minutes and below 1 min in seconds If you press the or key repeatedly you increase the corresponding preset parameter speed or run time stepwise If you press and hold down the chosen key the respective value changes continuously at first slowly and after a few seconds at an accelerated pace Air cooling of the Biofuge haemo During a run the spinning rotor creates frict...

Page 13: ...ining hematocrit values after removing the capillaries is delivered with the instru ment Reading spiral As an option you can order a reading spiral which is placed directly onto the rotor for reading hematocrit values in situ 1 3 4 5 6 7 8 9 1 0 1 1 1 2 1 3 1 4 1 5 1 6 1 7 1 8 1 9 2 0 2 1 2 2 23 24 H E R A E U S I N S T R U M E N T S C at No 1067 I I 1 2 0 0 0 R P M I I 1 2 3 4 5 6 7 8 9 1 0 1 1 1...

Page 14: ...rit rotor order no 7500 1067 for biofuge haemo number of samples 24 blood capillaries temperature range 4 C to 40 C maximum capacity 24 capillaries with 1 2 to 1 8 mm diameter and 75 mm length maximum speed nmax 12000 maximum RCF value at nmax 14926 ...

Page 15: ...n sleeves each are available on request With the adapt ers you can centrifuge all commercially available mi croliter tubes with volumes of 0 2 ml to 0 6 ml as well as 0 2 ml PCR reaction vessels Please refer to our sales documents for detailed infor mation on accessories including technical data and order numbers 1 2 3 4 5 6 7 8 9 1 0 1 1 1 2 1 3 1 4 1 5 1 6 1 7 1 8 1 9 2 0 2 1 2 2 23 24 max 13000...

Page 16: ...f places volume 24 x 1 5 2 ml maximum permissible load 24 x 4 g temperature range 4 C to 40 C maximum speed nmax min 1 13000 maximum RCF value at nmax 16060 minimum speed nmin min 1 2000 minimum RCF value at nmin 380 maximum radius cm 8 5 minimum radius cm 5 9 angle of incidence 40 maximum kinetic energy s 1 65 kNm ...

Page 17: ...0 6 ml 24 turquoise 7600 3758 reduction sleeve 6 x 46 mm 0 25 0 4 ml 24 red 7600 3759 Other accessories Reading harp 7600 0938 Reading spiral 7500 1236 For use with the fixed angle rotor also available fixed angle rotor 24 x 1 5 2 ml 7500 3325 special cap nut for fixing the rotor 10 mm tubular hexagon wrench for tightening the cap nut The printed documents consist of the delivery notes and this Ma...

Page 18: ...Accessories 14 for your notes ...

Page 19: ...cient air circulation a minimum dis tance from the wall of 10 cm at the back and of 15 cm on each side must be kept The centrifuge must be protected from heat and direct sunshine The location should be well ventilated Mains connection Make sure that your mains voltage and frequency match the specifications on the instrument Removing the transport protection Turn the instrument on The display panel...

Page 20: ...Before use 16 for your notes ...

Page 21: ...pply The instrument does not have a mains switch After you have established the mains connection the following reading appears in the control panel This tells you that the instrument carries out an internal check of its soft ware If the lid is closed the display in the panels speed and time changes to 0 after a couple of seconds With opened lid the speed panel displays the message OPEN instead If ...

Page 22: ...he brake does not function and braking takes much longer than normal Should it be necessary to open the lid manually carry out the following steps 1 Make sure the rotor stands still consult window in the lid 2 Unplug the mains plug 3 Push a thin screwdriver or another suitable tool horizontally from each side through the two openings in the side panels of the centri fuge see figure Push the lockin...

Page 23: ... lid and make sure that the rotor chamber is clean Remove eventual dust foreign material or sample residues 2 Turn the rotor so that the driving pin of the motor shaft fits into the groove on the underside of the ro tor and slide the rotor onto the drive shaft until the rotor hub rests on the shaft cf Figure 1 3 4 5 6 7 8 9 1 0 1 1 1 2 1 3 1 4 1 5 1 6 1 7 1 8 1 9 2 0 21 2 2 23 24 1 3 4 5 6 7 8 9 1...

Page 24: ...rry out the steps described above under Inserting the hema tocrit rotor in reverse order Irreparable damage to the motor Never tilt the rotor during removal 1 Open the centrifuge lid 2 Unscrew and remove the rotor cap 3 Loosen the rotor fastening nut counterclockwise using a coin Grab the rotor centrally at the hub and pull out perpendicularly Do not tilt The following description applies only to ...

Page 25: ...e fully Open container lids can damage the rotor lid For some special applications the container lids must remain unsealed If this is the case please use the screw top order no 75003326 instead of the standard snap on lid order no 70901111 A Position the snap on lid Press the rotor lid down onto the rotor until the snap on catch engages onto the ball of the acorn nut B Remove the snap on lid By ac...

Page 26: ...ed Possible damage to drive and rotor Do not push down the rotor by force If you cannot screw on the cap nut eas ily remove the rotor and reinsert it To put the rotor into position proceed as follows 1 Open the lid and make sure that the rotor chamber is clean Remove eventual dust foreign material or sample residues The thread and the O ring on the motor shaft must be in perfect condition 2 Turn t...

Page 27: ...cap nut as needed Permissible rotor temperature The rotor 7500 3325 may be used only within a temperature range of 4 C to 40 C Pre cooling in the freezer is not permitted Lifetime of the rotor There are no restrictions to the service life of the high performance rotor 7500 3325 B However please ob serve the following due to safety reasons Rotors and accessories made of plas tic should not be expos...

Page 28: ...n the lid of the centrifuge 2 Remove rotor cap not necessary with the hermetic lid 3 Screw the cap nut open by turning it counterclock wise using the socket wrench delivered with the in strument Remove the cap nut 4 Grab the rotor in the middle and pull gently upwards off the drive shaft Be careful not to jam it Loading the rotor As an accessory you can optionally order the fixed angle rotor 7500 ...

Page 29: ...alculated for a sample density of 1 2 g cm 3 If the density of your sample is higher you must either reduce its volume to an overall weight of 4 g or calculate a reduced speed nperm according to the following formula nperm n g cm max weight of tube with sample weight of tube with denser sample 1 2 3 Filling the tubes Separations by centrifugation function best when the unbalance of the rotor is mi...

Page 30: ...e screw on top Attention Please check that your sample containers are suitable for the centrifugal application desired 16060 x g temperature in uncooled devices approx 10 K above room temperature Please observe the permissible filling volumes Nominal volume Permissible volume 2 0 ml 1 5 ml 1 5 ml 1 0 ml others 2 3 nominal volume The sealing elements are to be checked regularly for damage to the sh...

Page 31: ... the amount of test liquid 0 5 sodium fluorescein in water specified in the col umn leakage test into the lower part of the rotor within a virtual circle comprising the vessel bores not the bores themselves using a pipette or sy ringe Place the rotor lid on top and screw it on ATTENTION Make sure that there is no spilled test liquid on the rotor clean if necessary Carry out a test run for 10 minut...

Page 32: ...r loading Improper loading can in the worst case lead to damage to rotor and centrifuge Unbalance not only causes a noisy run but rapidly damages the motor suspension After loading the rotor snap on the rotor cap until it locks into place The optional hermetic lid 75003326 is screwed onto the rotor with the central nut Close the lid of the centrifuge by firmly pressing it down There must be a clic...

Page 33: ...s thus indicating that you can change the setting in multiples of 100 rpm 2 Press one of the set keys for an increase or for a decrease By pressing the selected key briefly you increase or decrease the speed in steps of 100 rpm This option is supposed to be used for small changes and fine tuning 3 If you keep the key pressed the display changes at first slowly and after a few seconds at an acceler...

Page 34: ...y briefly you increase or decrease the preset run time in steps of 1 min This option is supposed to be used for small changes and fine tuning 3 If you keep the key pressed the display changes at first slowly and after a few seconds at an acceler ated pace 4 Release the key as soon as you are close to the desired value and fine tune if necessary by repeat edly pressing the selected key or its count...

Page 35: ...ting Press the start stop key in the control panel The dis play panel shows the preselected values for speed and run time see Figure below The centrifuge accelerates to the preselected value Simultaneously the run time display starts going backward from the preset time giving the remaining run time in minutes After reaching the last minute the display switches to seconds remain ing If you have cho...

Page 36: ... during a run by pressing consecutively set in the time panel or and again set Displaying the preset values You can check the preset values for speed and run time by pressing one of the set keys If you press the key again the display turns dark Stopping the centrifuge Stopping with preset time Normally the run time has been preselected and all you have to do is wait until the centrifuge terminates...

Page 37: ... By pressing the lid key you can now open the lid and remove your samples The speed panel displays OPEN After 2 minutes the instrument switches to the standby mode and all displays turn dark You can even now open the lid by pressing the lid key You can stop the centrifuge at any time by pressing the stop key Biofuge haemo set set 0 speed time lid quick run start stop INSTRUMENTS lid quick run star...

Page 38: ...available whenever the LED next to the quick run key is lit The preset speed is ignored Centri fuge tubes and rotor may be dam aged Check carefully whether you have to maintain a specific speed Never use this option for hematocrit measurements When you press and hold the quick run key the cen trifuge accelerates with full power up to the maximum speed of 13 000 rpm unless you release the key The p...

Page 39: ...your tubes are designed for such a strain and reduce the speed if necessary The maximum RCF value refers to the maximum radius of the tube For the fixed angle rotor 7500 3325 the above formula yields for the bottom of the bore radius 8 5 cm a maximum RCF value of 16 060 RZB 1118 13 000 1000 8 5 16 060 2 Please note that this value becomes lower depending on the tubes and adapters used You may take...

Page 40: ...it dish which may remain in the centrifuge Using the outer knurled eccentric screw turn the zero line so that it comes to lie on the outer end of the blood col umn then place the 100 line of the reading spiral over the meniscus using the knurled screw in the mid dle You can now read the hematocrit value directly from above on the graduation Once you are finished you can dispose of all capillaries ...

Page 41: ...Operation 37 for your notes ...

Page 42: ... make sure by consulting the manufacturer that the procedure foreseen does not cause any dam ages to the instrument Cleaning Pull mains plug before cleaning the instrument Clean the casing the rotor chamber the rotor and the accessories regularly and in case of need This is indi cated both for reasons of hygiene and to prevent corro sion due to contamination sticking to the instrument and its acce...

Page 43: ...ing a run you have to disinfect the centrifuge im mediately Infectious material could enter the centri fuge if spills or tube breakage occur Danger of infection may occur upon contact Take appropriate protective meas ures for personnel Mind the permissible filling volumes and loading limits for the tubes In case of contamination the operator has to make sure that no further persons are jeopardized...

Page 44: ...nent safety procedures for handling infec tious material 1 Pull mains plug 2 Unscrew the rotor chuck 3 Grab the rotor with both hands and pull it perpen dicularly off the drive shaft 4 Remove the centrifuge tubes and adapters and disinfect them or dispose of them as necessary 5 Treat the rotor and the rotor lid according to the instructions given for the disinfectant soaking in liq uid or spraying...

Page 45: ... the rotor should not be warmer than ca 25 C 2 Do not let the bleaching solution act longer than absolutely necessary 3 After disinfection rinse the rotor thoroughly with distilled water and allow to dry Decontamination For general radioactive decontamination use a solution of equal parts of 70 ethanol 10 SDS and water Follow this with ethanol rinses then de ionized water rinses and dry with a sof...

Page 46: ...7500 3325 maximally 10 times The rotor must be cleaned and rinsed with distilled water before being autoclaved Remove the rotor lid the centrifuge tubes and the adapters Place plastic rotors on an even surface to avoid deformation Chemical additives to the steam are not permitted Never exceed the maximum permis sible values for autoclaving tempera ture and autoclaving time Should the rotor show si...

Page 47: ...vering it Inspection costs are charged as flat rate contracts Necessary repairs are carried out free of cost within the warranty conditions and against payment after expira tion of the warranty period Warranty conditions The warranty period starts with the day of delivery Within the warranty period the centrifuge is repaired or replaced free of cost if there are provable faults in ma terials or wo...

Page 48: ...s connection 2 If the mains connection is OK call the nearest Service Displays fail briefly The motor stops suddenly The rotor stops without braking The display reads br see br Brief interruption of mains supply 1 Check whether the plug is plugged in properly 2 Restart the centrifuge Lid cannot be opened Pressing the lid key has no effect Lid not correctly engaged or lid warped Press lid down in t...

Page 49: ...ice br Mains connection interrupted during run If the plug was pulled inadvertently plug in again Wait for about 75 seconds The centrifuge comes to a stop without braking E 1 or E 12 Rotor does not start Pull mains plug and plug in again If the error persists call Service E 3 No braking current drive out of order rotor stops without braking Lid opened during run Wait until the rotor has come to a ...

Page 50: ... lid lock mechanism The lock must be engaged on both sides If error persists call nearest Service Flashing red point in time panel Displays remain dark Not an error instrument is on standby Press any key With start stop or quick run the centrifuge starts running at once With the lid key the lid pops open and the display reads OPEN In case you must call the Service Should you require our Service pl...

Page 51: ...Troubleshooting 47 ...

Page 52: ...c Lid stove enameled steel plate Keys and display panel Keys and display panel covered with easy care protective foil Rotor chamber Material impact resistant plastic Dimensions diameter x height 222 mm x 110 mm Lid release Electromagnetic release with lid key when connected to mains Lid lock Automatic locking when the lid is pressed shut Emergency lid release Lid release in case of power failure e...

Page 53: ...ring the run End of centrifugation The panels speed and time read 0 parameter memory parameter display speed run time Speed selection adjustable in steps of 100 min 1 in the range of 1600 min 1 to 13000 min 1 Run time selection adjustable in minutes from 1 min to 99 min hd mode continuous operation Time display in quick run mode between 1 s and 60 s in seconds over 1 min in minutes diagnostics lid...

Page 54: ...f the environ ment 4 C to 35 C during operation 10 C to 50 C during storage and transport dimensions 240 mm x 284 mm x 375 mm H x W x D weight without rotor 14 5 kg maximum speed 13 000 rpm hematocrit rotor 12 000 rpm maximum RCF value 16 060 fixed angle rotor 14 926 hematocrit rotor minimum speed 1600 rpm minimum RCF value 209 noise 64 dB A maximum capacity 24 x 2 ml maximum permissible load 24 x...

Page 55: ...cked in accordance with EN 61 010 1 EN 61 010 2 020 EN 50 081 1 EN 50 082 1 Electrical connections fuses Order no Voltage Frequency max current Power consumption Fuses inside instrument 7500 3635 230 V 50 60 Hz 1 25 A 170 W 2 x 6 3 AT 5 x 20 mm 7500 3557 120 V 50 60 Hz 2 5 A 170 W 2 x 6 25 AT 6 3 x 32 mm 1 x 10 AT 6 3 x 32 mm ...

Page 56: ...52 Speed g diagram for rotor 7500 3325 10 100 1000 10000 100000 RZB x g RCF x g Drehzahl 1 min speed rpm 100 1000 10000 100000 13000 16060 r 5 9 cm r 8 5 cm min max ...

Page 57: ...53 Autoclaving protocol only fixed angle rotor 7500 3325 Date Remark Operator Signature 1 2 3 4 5 6 7 8 9 10 ...

Page 58: ...fuge starting 31 centrifuge tubes 8 cleaning 37 conditions of warranty 42 contamination necessary measures 38 continuous operation 30 33 control panel readings 8 corrosive substances protective vessels for 4 D damage symbol for potential 5 dangerous chemicals 3 decontamination 38 density maximum of sample 25 disinfectant 39 disinfection 4 procedure 39 disinfection with bleaching lye 40 display dur...

Page 59: ...luorescein test solution for aerosol tightness 27 fuses 50 H hazardous substances 3 hazards symbols used for 5 hematocrit measurement 35 standardization 35 hematocrit determination quick run function not to be used 33 hematocrit dish standard equipment 9 hematocrit rotor fastening 20 insertion 19 loading 19 removal 20 technical data 10 hints symbol for 5 I icons for denoting dangers and potential ...

Page 60: ...present 17 maintenance 37 manual lid release 18 steps for 18 maximum sample density 3 maximum sample volume 8 O operation continuous 30 33 preselected run time 30 short time 33 organic solvents not allowed for cleaning 38 original parts mandatory use 4 overloading dangers implied 25 P partial loading of rotor 28 pathogenic microorganisms protection against 4 permissible volume calculation of for d...

Page 61: ...ory 9 reading spiral 13 for hematocrit 35 optional accessory 9 readings of control panel during run 8 relative centrifugal force 34 rotor approved 22 insertion 22 loading 28 partial loading 28 removal 24 rotor cap 23 24 mandatory fastening 20 rotor chamber technical data 47 rotor insertion tools for 22 run time fine tuning 30 for hematocrit measurements 30 range 30 setting 30 running noise 44 RZB ...

Page 62: ...tness 27 software check internal 17 speed fine tuning 29 range 29 setting 29 speed of centrifugation for calculation of RCF value 34 standardization of hematocrit measurements 35 standby mode 7 start key 31 starting run 31 stopping 32 substructure 15 symbols for hazards and dangers 5 T temperature regulation 8 tools for inserting rotor 22 toxins protection against 4 transport precautions for 17 tr...

Page 63: ...59 calculation of permissible v for dense samples 25 volume of sample maximum 8 W warning lid open during run 7 warranty conditions 42 ...

Page 64: ...rica Kendro Laboratory Products International Sales Asheville NC USA Tel 1 828 658 2711 Fax 1 828 645 9466 info kendro spx com North America Kendro Laboratory Products Asheville NC USA Tel 1 800 879 7767 Fax 1 828 658 0363 info kendro spx com Internet http www kendro com Kendro Laboratory Products GmbH Robert Bosch Straße 1 D 63505 Langenselbold Telephone 49 0 6184 90 6940 In the interest of conti...

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