NanoPhotometer
®
N120/
NP80/N60/N50/C40
User Manual Version 4.3.1
53
Note
: Do not overfill the well.
Note:
A new Blank is recommended when changing the dilution lid.
6. For measurements, ensure that the lid fits exactly onto the positioning supports mounted
to the body of the cell and initiate a blank measurement with the blank button.
Note
: Possible UV exposure. Only initiate a measurement when the lid is closed.
7. Clean the measurement window and mirror in the lid with a slightly wet lint-free tissue.
Use water, 70% EtOH or isopropanol, if needed.
Note
: Do not use aggressive solvents such as strong acids or bases or organic solvents
at any time (see page 55 solvent compatibility). If unsure please contact
for detailed information about your specific reagent/solvent.
8.
It is possible to enter a sample name for each sample in the input window “enter sample
name”.
Note
: Allowed characters are: A…Z a…z 0…9 , . - ( ) @ ! = _ ~ ; [ ] { } ‘
Note:
Blank character is not allowed.
9. Mix the sample very well to achieve a homogenous sample.
10. Apply the appropriate amount of sample solution onto the measurement window and
initiate the sample measurement with the sample button. Upon completion of
measurement remove the lid, clean the surfaces and apply the next sample.
S
AMPLE
H
ANDLING
T
IPS
NanoVolume Methods (NP80/N60/N50)
The NanoPhotometer
®
includes an integrated vortexer (N60/NP80 only) to assure
sample homogeneity. It is recommended to vortex every sample right before the
measurement.
The sample window on pedestal is illuminated (NP80/N60/N50 only) with a low energy
red light to assist with accurate sample application. The red light is switched off once the
lid arm is closed. It is possible to disable the illumination feature in preferences of the
NPOS software.
The minimum volume that can be used for NanoVolume samples is 0.3 µl
(NP80/N60/N50 dsDNA > 420 ng/µl and BSA > 12.6 mg/ml and for submicroliter cell in
the C40 dsDNA > 25 ng/µl and BSA > 0.7 mg/ml). For automatic path length setting at
least 1µl is needed (NP80/N60/N50 only).
The maximum volume that can be used for NanoVolume samples is 2.0 µl
(NP80/N60/N50) and 5 µl for the submicroliter cell with lid 5.
The sample can be fully recovered after measurement with a pipette if desired.
Note:
Minimal cross contamination cannot be avoided on molecular level.
Proper cleaning is important to ensure accurate measurements. In most cases a dry lint-
free laboratory wipe is sufficient to clean the sample quartz surfaces. In the case of