NanoPhotometer
®
N120/
NP80/N60/N50/C40
User Manual Version 4.3.1
105
M
ORE
A
PPS
:
W
AVESCAN
M
ETHOD
O
VERVIEW
Using the wavescan application it is possible to obtain the complete spectral scan for a
defined wavelength range between 200-900 nm (NP80/N60/C40) or from 200-650 nm (N50).
M
EASUREMENT
P
ROTOCOL
S
INGLE
S
AMPLE
1. Select the More Apps icon from the Home screen and the Wavescan
icon from the More Apps screen
2.
To change between NanoVolume and Cuvette application, use the
Change to Cuvette/NanoVolume button below the parameter area
(NP80/C40 only).
3.
For NanoVolume application:
Select the dilution depending on the sample concentration
Note
: There is no automatic path length setting in this method. Select either a virtual
dilution of 15 (path length 0.67 mm) or of 140 (path length 0.07 mm)
For cuvette application:
Select the path length depending on the used cuvette.
Options are: 0.5 mm, 1 mm, 2 mm, 5 mm and 10 mm
If it is desired to heat the sample to 37°C use the toggle switch to turn on cell holder
heater. When the cuvette holder has reached 37°C the toggle color changes to green.
Note
: Only available for cuvette applications (NP80 and C40).
For NanoVolume application with submicroliter cell (C40 only):
Select Lid depending on your sample concentration (for concentration ranges of different
lids see page 52 Submicroliter Cell Measurements Basics).
Required sample volume for each lid is shown.