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Implen NanoPhotometer C40 User Manual Download Page 143

NanoPhotometer

®

 

N120/

NP80/N60/N50/C40

 

User Manual Version 4.3.1

 

 

143 

 

L

ICENSE 

I

NFORMATION 

 

The NPOS software is Copyright of Implen and its affiliates. The NPOS software includes 
some open source software components under the licenses listed. 

E

ND

-U

SER 

L

ICENSE 

A

GREEMENT 

(EULA) 

Shows the end-user license agreement. 

T

RADEMARKS

 

Windows is a trademark of Microsoft. Airprint, Mac OS, OS X, iOS, iPhone and iPad are 
trademarks of Apple. Android OS is a trademark of Google. Linux is a trademark of Linus 
Torvalds. 

 

 

Summary of Contents for NanoPhotometer C40

Page 1: ...NanoPhotometer N120 NP80 N60 N50 C40 User Manual Version 4 3 1 Software Version 4 3 15670 ...

Page 2: ...e terms and agrees that Implen shall not be held liable for any loss deletion or damage of any data or files for any reason including any damages attributable thereto Telephone support is available using one of the following phone numbers from your geographic region Europe Asia South Pacific North and South America Middle East Africa Phone 49 89 72637180 Phone 1 818 748 6400 Fax 49 89 726371854 Fa...

Page 3: ...duct FCC 47 CFR Part15 15 107 and 15 109 EN 301 489 1 V1 9 2 Radio and ancillary equipment for portable use portable equipment EUT Operating frequency range 2 4 2 4835 GHz EN 300 328 V1 8 1 Electromagnetic compatibility and Radio spectrum Matters ERM Wideband transmission systems Data transmission equipment operating in the 2 4 GHz ISM band and using wide band modulation techniques Harmonized EN c...

Page 4: ...ATIONS 22 2 GETTING STARTED 24 SPECTROPHOTOMETER INSTALLATION 24 SAFETY INFORMATION 24 UNPACKING AND POSITIONING 25 SOFTWARE INSTALLATION 26 NPOS OVERVIEW 26 REQUIREMENTS AND COMPATIBILITY 26 INSTALLING SOFTWARE ON COMPUTER 27 INSTALLING NANOPHOTOMETER APP ON TABLET OR SMARTPHONE 28 FIRST STEPS AND CONFIGURATION WIZARD 29 PRINTER INSTALLATION 29 3 NANOPHOTOMETER BASICS 30 APPLICATIONS OVERVIEW 30 ...

Page 5: ...TIONS 65 PROTEIN UV 70 METHOD OVERVIEW 70 MEASUREMENT PROTOCOL SINGLE SAMPLE 71 MEASUREMENT PROTOCOL MULTI SAMPLE 74 CALCULATIONS 78 PROTEIN ASSAYS 84 METHOD OVERVIEW 84 MEASUREMENT PROTOCOL SINGLE SAMPLE 85 MEASUREMENT PROTOCOL MULTI SAMPLE 88 SAVING AND LOADING STANDARD CURVES 93 CALCULATIONS 93 KINETICS 94 METHOD OVERVIEW 94 MEASUREMENT PROTOCOL 94 CALCULATIONS 96 OD600 97 METHOD OVERVIEW 97 ME...

Page 6: ...RESULTS 122 STORED METHODS 123 5 PREFERENCES 124 GENERAL 124 DATE AND TIME 124 DISPLAY 125 ABOUT 125 STORAGE 125 LANGUAGE 125 ILLUMINATION SAMPLE WINDOW 126 ENABLE NANOVOLUME 126 DYES 126 WARNING MESSAGES 128 BLANK CONTROL 128 SAMPLE QUALITY CONTROL 128 NETWORK 129 NETWORK SETTINGS 129 WLAN SETTINGS 130 FILE SERVER ACCESS 131 NETWORK FOLDER 132 PRINTER 133 NETWORK PRINTER 133 REPORT CONFIGURATION ...

Page 7: ...NTENANCE FREE TECHNOLOGY 145 REPLACEMENT PARTS 145 CLEANING AND GENERAL CARE 146 9 WARRANTY 147 10 APPENDIX 1 INSTALACE SPEKTROFOTOMETRU 148 BEZPEČNOSTNÍ INFORMACE 148 VYBALENÍ A UMÍSTĚNÍ 149 11 APPENDIX 2 INSTALLATIE SPECTROFOTOMETER 150 VEILIGHEIDSINFORMATIE 150 UITPAKKEN EN PLAATSEN 151 12 APPENDIX 3 INSTALLATION DU SPECTROPHOTOMÈTRE 153 INFORMATIONS DE SÉCURITÉ 153 DÉBALLAGE ET POSITIONNEMENT ...

Page 8: ...ZPAKOWYWANIE I UMIEJSCOWIENIE 163 16 APPENDIX 7 INSTALACIÓN DEL ESPECTROFOTÓMETRO 165 INFORMACIÓN DE SEGURIDAD 165 DESEMBALAJE E INSTALACIÓN 166 17 APPENDIX 8 SPEKTROFOTOMETERINSTALLATION 168 SÄKERHETSINFORMATION 168 UPPACKNING OCH PLACERING 169 18 APPENDIX 9 INSTALAÇÃO DE ESPECTROFOTÓMETRO 171 INFORMAÇÕES DE SEGURANÇA 171 DESEMBALAMENTO E POSICIONAMENTO 172 19 ALPHABETICAL APPENDIX 174 ...

Page 9: ... technology squeezes the sample between two quartz surfaces allowing for unmatched precision and accuracy without the need for dilutions Combined with our True Path Technology the system offers lifetime accuracy and precision without the need for maintenance or recalibration Note It is recommended to use a properly calibrated pipette with high quality tips to ensure delivery of appropriate sample ...

Page 10: ...NanoPhotometer N120 NP80 N60 N50 C40 User Manual Version 4 3 1 10 NANOPHOTOMETER N120 Item Number Touchscreen Battery Pack N120 Touch N120 Mobile ...

Page 11: ...NanoPhotometer N120 NP80 N60 N50 C40 User Manual Version 4 3 1 11 NANOPHOTOMETER NP80 Item Number Touchscreen Battery Pack NP80 NP80 Touch NP80 Mobile ...

Page 12: ...NanoPhotometer N120 NP80 N60 N50 C40 User Manual Version 4 3 1 12 NANOPHOTOMETER N60 Item Number Touchscreen Battery Pack N60 N60 Touch N60 Mobile ...

Page 13: ...NanoPhotometer N120 NP80 N60 N50 C40 User Manual Version 4 3 1 13 NANOPHOTOMETER N50 Item Number Touchscreen Battery Pack N50 N50 Touch ...

Page 14: ...NanoPhotometer N120 NP80 N60 N50 C40 User Manual Version 4 3 1 14 NANOPHOTOMETER C40 Item Number Touchscreen Battery Pack C40 C40 Touch C40 Mobile ...

Page 15: ... push 3 seconds initiate a hard reset Only activate a hard reset of the NanoPhotometer when necessary To avoid unnecessary hard reboots it is recommended to power down the unit from the onboard touch screen by pushing on the power button in the bottom left corner of the home screen INSTRUMENT BOTTOM VIEW N120 NP80 N60 N50 C40 Model name device serial number and FCC ID are located on the identifica...

Page 16: ...stallation NanoPhotometer Power Adapter Power adapter for the NanoPhotometer and for charging the optional built in battery pack Note Use only the power adapter supplied with your instrument or a replacement part from the manufacturer or your supplier Dust Cover OPTIONAL ACCESSORIES Didymium Glass Filter C40 NP80 The certified didymium glass filter can be used to verify the wavelength and photomet...

Page 17: ...for the NanoPhotometer N50 Submicroliter Cell C40 only The submicroliter cell provides the option of upgrading the NanoPhotometer C40 to a device that handles NanoVolume samples as well as cuvette samples The sample volume range of the submicroliter cell ranges from a minimum of 0 3 µl to 5 µl with the path length options including 0 04 mm 0 1 mm 0 2 mm 1 mm and 2 mm which correspond to virtual di...

Page 18: ... be shown in the input window The imported name can then be edited or replaced completely Barcode readers that have been tested and verified compatible 1D Honeywell Voyager 95X0 Single Line Laser Scanner Datalogic Touch65 1D 2D AGPtEK SC36 Honeywell Xenon 1900g DYMO Label Printer It is possible to connect a DYMO Label printer to the NanoPhotometer for direct printing on standard or cryo labels Rec...

Page 19: ...th AirPrint IPP compatible printers supporting PDF format For network printer settings see page 133 Network Printer Note IPP version 2 2 is required and some printer configuration settings might need to be changed in order to allow communication with the NanoPhotometer The following HP printers have been tested and deemed compatible to print via USB connection HP LaserJet 3030 HP LaserJet m1522nf ...

Page 20: ...rnet LAN connection port on the rear panel of the instrument which enables the NanoPhotometer to connect with the local network This Ethernet connection can be used for data transfer from the NanoPhotometer to a local network to control the NanoPhotometer via a control device and network printing Data transfer is possible by saving on a defined network folder see page 132 Network Folder or on the ...

Page 21: ...s device is limited to 40 measurements per dataset Larger datasets can be saved on the NanoPhotometer itself HDMI There is an HDMI port located on the rear panel of the NanoPhotometer which is compatible with HDMI 1 4 cables or better to connect the NanoPhotometer to HDMI compatible monitors Note The maximum HDMI cable length is 5 meters ...

Page 22: ... 800 rpm tube size up to 2 0 ml Cuvette Performance NP80 C40 Detection Range dsDNA 0 1 130 ng µl Detection Range BSA 0 003 3 7 mg ml Photometric Range 0 2 6 A Center Height Z Height 8 5 mm Cell Types outside dimension 12 5 x 12 5 mm Heating 37 C 0 5 C Optical Specifications Wavelength Scan Range C40 N60 NP80 N120 200 900 nm N50 200 650 nm Measure Time For Full Scan Range C40 N50 N60 NP80 2 5 4 0 s...

Page 23: ...tel x86 und Apple M1 iOS and Android OS General Specifications Main Body Size 200 mm x 200 mm x 120 mm Weight 3 8 5 2 kg depending on configuration Operating Voltage 90 250 V 10 50 60 Hz 90 W 18 19 VDC Display 1024 x 600 pixels touchscreen glove compatible Built in Battery Pack Optional rechargeable lithium ion battery C40 N60 NP80 95 Wh 6 6 Ah 8 h N120 47 5 Wh 3 3 Ah 3 h Min charging cycles 800 C...

Page 24: ...iter cell is used with the NanoPhotometer C40 make sure that the lid is placed on the measurement head prior to the measurement WARNING UV exposure when measuring without lid Do not use damaged power cords accessories and other peripherals with your NanoPhotometer Use only the delivered and specified power supply charger Do not expose the NanoPhotometer to strong magnetic electrical fields water c...

Page 25: ...d in transit If any damage is discovered inform your supplier immediately Ensure your proposed installation site conforms to the environmental conditions for safe operation indoor use or dry environment Note Do not expose your NanoPhotometer near liquids chemicals rain moisture or dusty environments Working temperature range 10 40 C NP80 N60 N50 and C40 and 10 32 C N120 If the cuvette heating is u...

Page 26: ...ipment may be impaired and the instrument warranty voided SOFTWARE INSTALLATION NPOS OVERVIEW NPOS is a Linux based operating system designed for the NanoPhotometer NPOS can store data either to a common directory or be configured to save to independent directories according to file format and or instrument NPOS can save data in an Implen IDS format PDF or as an Excel format file Note PDF and Exce...

Page 27: ...he new software version Note The Windows and Mac installation files are located on the Implen USB flash drive which is included with the NanoPhotometer at time of delivery The files are available for free download at any time in the download area of the Implen website www implen de downloads NPOS installation for single multi user on Windows computer 1 Check the installed version of the NanoPhotom...

Page 28: ...a installed on your computer it is recommended to switch off the browser safety This may interfere with the NPOS running on your computer INSTALLING NANOPHOTOMETER APP ON TABLET OR SMARTPHONE The NanoPhotometer App can be installed as an application on tablets and smartphones with compatible Android and iOS operating systems see page 26 Requirements and Compatibility The NanoPhotometer App is avai...

Page 29: ...t time an Implen configuration wizard is shown Please accept the End User License Agreement EULA and select the country in which the NanoPhotometer is used and confirm PRINTER INSTALLATION For printers connected via USB connection 1 Switch NanoPhotometer on home screen 2 Connect DYMO HP printer via USB cable 3 DYMO HP printer is ready to use after 30 seconds Note Make sure that the home screen is ...

Page 30: ...l NP80 N60 N50 and 2 µl for the NanoPhotometer N120 Standard cuvette applications can be performed with 10 mm 5 mm 2 mm 1 mm and 0 5 mm path length quartz glass or plastic cuvettes with a center height of 8 5 mm APPLICATIONS OVERVIEW The NanoPhotometer comes with pre programmed applications as well as the ability to create custom applications To select a method tap the corresponding icon and the m...

Page 31: ...s found on a secondary method screen Wavelength Define one or multiple wavelength between 200 900 nm N50 200 650 nm for absorbance measurements Wavescan Define desired full scan range anywhere between 200 900 nm N50 200 650 nm Concentration Define extinction coefficient for automatic concentration calculations Absorbance Ratio Define two wavelengths absorbance ratio calculation Standard Curve Crea...

Page 32: ...available Delete Data Opens a delete dialog pop up Sample ID Opens sample ID pop up to define sample IDs N120 only Graph Opens graph overlay for multi channel mode N120 only Parameter Opens parameter window Results Opens result window Graph Opens graph result window Table Open shows results in table format Sample Map Opens sample map window for computer versions N120 only Standard Map Opens standa...

Page 33: ...able after updating an older firmware version The Change to Cuvette button NP80 is located at the bottom of the parameter window The change to Cuvette NanoVolume button toggles between NanoVolume and cuvette applications Selecting the Change to Cuvette button activates the cuvette compartment opens the sliding cover and switches the red arrow LED on Only cuvette measurements are possible when this...

Page 34: ...ere is no table area available for smartphones Parameter area In the parameter area it is possible to define all necessary parameters for a measurement as well as turn on cuvette mode and initiate cuvette heating The standard measurement screen shows the parameter area open by default The parameter area is automatically hidden when starting either a blank or sample measurement by pushing on the Bl...

Page 35: ...he overlay option is enabled the graphs of the measurements will be automatically overlaid To change the overlaid graphs use the tick boxes in the table Note It is only possible to overlay up to 30 graphs in a chart If more than 30 data are selected a message will appear that says More than 30 samples have been selected Only 30 will be shown in graph Note The overlay button is not available on the...

Page 36: ...n is a screen carousel that shows the active and available screens For computer versions without touchscreen a vertical tab bar is shown to change between the different screens parameter sample map results and graph for Protein Assay additionally standard map and standard curve Sample Map Definition of Sample IDs The sample map screen shows the sample IDs as defined or names it automatically if no...

Page 37: ...tion Options Selection of cells is only possible in the sample map screen Cell A01 must always be selected A push in the top left corner selects deselects all 96 cells at once Manual selection is possible by selecting any area starting in cell A01 or by pushing in any cell ...

Page 38: ...mple IDs are defined the Sample IDs are named automatically To define sample IDs open the Sample ID pop up on the Sample Map screen The Sample ID pop up offers options to set sample IDs delete sample IDs import or export sample IDs from a csv file To set sample IDs select Blank BLK Sample SPL or Control CRL and push on Set IDs button Sample IDs are numbered automatically To delete sample IDs close...

Page 39: ...ltaneously open the table view after a measurement and select Edit Sample IDs can be imported with the Import function For the import of Sample IDs the sample IDs must first be defined in the following format and saved as a csv file for horizontal Sample Map Note Draft files are available for download on the Implen homepage www implen de downloads for vertical Sample Map ...

Page 40: ...er a file name select a folder and confirm with export Result Screens There are multiple result screens available depending on parameter setting and method Nucleic Acids Concentration Graph Ratio A260 A280 Ratio A260 A230 A260 Protein UV Concentration Graph Ratio A260 A280 A280 If a dye is added three additional screens are shown Dye Concentration Dye Absorbance FOI DOL Protein Assay Standard Map ...

Page 41: ...sult cell opens a single result overview A double click to a result cell shows tick boxes for all measured samples The tick boxes can be used in the graph screen to overlay up to 12 samples Up to 12 ticked samples will be overlaid by pushing on the graph icon in the navigation bar ...

Page 42: ...measured samples With the edit button selected Sample IDs can be changed DATA PROCESSING DIALOGS PRINT Selecting the print icon opens a full screen dialog window with various print options The print icon is only shown if a printer is available The print command is sent primarily to DYMO or HP printers if they are directly connected to ...

Page 43: ... NanoPhotometer Note The print option is not available in smartphone apps Auto Print If the auto print function is enabled each measurement will be printed directly after the measurement Auto print function is available for DYMO printer HP printer connected via USB cable and network printer Note Default setting for auto print is off If enabled in one method it is set default on for all methods and...

Page 44: ...ed in the NanoPhotometer storage defined network folder or on a USB flash drive It is not possible to save IDS files from opened data In these cases the IDS tick box is greyed out Note PDF and Excel files cannot be opened on the NanoPhotometer The files need to be transferred to a computer or device where Excel or PDF reader is available Note All data saved on the NanoPhotometer are stored on an i...

Page 45: ...Photometer USB flash drive if connected Network folder if defined and Control Device If Control Device is selected the data will be transferred to the control device that is currently connected with the NanoPhotometer Note It is not possible to save IDS files to a control device like computer PC Mac tablets or smartphones Auto Save To prevent data loss all measurements are automatically stored as ...

Page 46: ...will be deleted The header tick box selects deselects all samples Initiate the deletion with the delete button Confirm the deletion in the following warning message Do you want to delete all selected files select cancel x will return to the delete menu screen or confirm with delete to delete the selected data Note The delete function is not available for the software version designed for smartphon...

Page 47: ...thods Select the desired parameter settings and open the Stored Method dialog by pushing o the favorite icon Enter a method name and select a save location in the folder Options include NanoPhotometer USB flash drive if connected and Network folder if defined Push the store button to save the method Stored Methods can be opened on the homescreen by opening Stored Methods see page 123 Stored Method...

Page 48: ...lable with the optional accessory submicroliter cell from Implen NANOVOLUME SINGLE SAMPLE MEASUREMENT BASICS N50 N60 NP80 1 Select a method depending on your sample and set the parameters for the measurement 2 Ensure that the sample window on pedestal and the mirror in the lid arm are clean 3 Use the integrated vortexer N60 and NP80 only to mix the sample to achieve a homogenous sample 4 Raise the...

Page 49: ...arameter setting Volume 1 2 µl adjusts the path length automatically The parameter setting Volume 0 3 µl measures only the 0 07 mm path length for higher concentrations dsDNA 420 ng µl BSA 12 6 mg ml Note The sample window on pedestal must be clean and residual fluff from any cleaning wipe must be removed for optimum performance NANOVOLUME MULTI SAMPLE MEASUREMENT BASICS N120 Note The multi sample...

Page 50: ...7 Clean the measurement spots and mirrors in the lid arm with a slightly wet lint free tissue Use water 70 ethanol or isopropanol if needed Note Make sure that the metal contact face around the measurement window and the mirror is clean Note Do not use aggressive solvents such as strong acids or bases or organic solvents at any time see page 55 solvent compatibility If unsure please contact suppor...

Page 51: ... on your sample and set the parameters for the measurement 2 Open the automatic cuvette cover by pushing the Change to Cuvette button below the parameter area NP80 only 3 Add a blank solution to a cuvette and ensure that the filling volume is sufficient to allow light path to pass through the solution 4 Insert cuvette into the cell holder 5 Initiate a blank measurement with the blank button Upon c...

Page 52: ...ume option We guarantee our submicroliter cell specifications only for Implen submicroliter cells which are aligned to the NanoPhotometer C40 by Implen 3 Set the parameter for the measurement Choose the dilution lid depending on your sample concentration Lid dsDNA ng µl BSA mg ml absorbance range 10 mm path 5 optional 2 375 0 05 7 5 10 5 750 0 15 22 5 0 10 15 50 25 3 750 0 75 111 0 50 150 100 opti...

Page 53: ...with the sample button Upon completion of measurement remove the lid clean the surfaces and apply the next sample SAMPLE HANDLING TIPS NanoVolume Methods NP80 N60 N50 The NanoPhotometer includes an integrated vortexer N60 NP80 only to assure sample homogeneity It is recommended to vortex every sample right before the measurement The sample window on pedestal is illuminated NP80 N60 N50 only with a...

Page 54: ...illumination feature in preferences of the NPOS software e g 12 samples selected For blank and sample measurements 2 µl of sample volume are necessary Pathlength will be set automatically if necessary Proper cleaning is important to ensure accurate measurements In most cases a dry lint free laboratory wipe is sufficient to clean the sample quartz surfaces In the case of highly concentrated samples...

Page 55: ...LL Most solvents typically used in life science laboratories are compatible with the NanoPhotometer NanoVolume sample surfaces The following solvents are compatible for use with the NanoPhotometer models N50 N60 N120 and NP80 at room temperature Acetone 5 Acetonitrile Benzene Butanol Carbon tetrachloride Chloroform Ethanol Ether HEPES Hexane Isopropanol MES Methanol Methylene chloride MOPS Phenol ...

Page 56: ...e NanoPhotometer are connected to the same LAN WLAN network For connection of the NanoPhotometer to LAN WLAN see page 129 Network For Windows computer open the Windows explorer and enter the serial number or the NanoPhotometer IP in the address bar of the Windows Explorer e g M80798 or Assigned IP Address Note Serial number and IP address of the NanoPhotometer can be found in the NanoPhotometer so...

Page 57: ... influence the time for battery operation To prolong battery operation switch the screen saver off When the battery gets low there will be a first warning message Battery low and a beep is sounded At this status it is possible to use the instrument under standard conditions for at least one hour It is recommended to charge the battery at this status If the battery is not charged there will be a se...

Page 58: ...f labeling of nucleic acids with probes including fluorescent dyes Sample Control gives useful information about sample conditions It recognizes air bubbles sample impurities turbidity lint residues and potential contaminations If Sample Control detects any inconstancy an alert icon is shown in the result table area A push on the alert icon shows additional information about the inconstancy MEASUR...

Page 59: ... the used cuvette Options are 0 5 mm 1 mm 2 mm 5 mm and 10 mm If it is desired to heat the sample to 37 C use the toggle switch to turn on cell holder heater When the cuvette holder has reached 37 C the toggle color changes to green Note Only available for cuvette applications NP80 and C40 For NanoVolume application with submicroliter cell C40 only Select a Lid depending on your sample concentrati...

Page 60: ...ckground correction can be disabled with the toggle switch 7 Air bubble recognition is disabled by default Setting to on detects air bubbles lint residues and bad sample conditions of the sample Note Lint residues and bad sample conditions are detected even if the air bubble recognition is set to off 8 For dye labelled samples tick the dye labels in the list which should be used for result calcula...

Page 61: ...he illumination of the sample window can be switched off in the preferences 11 Use a lint free laboratory wipe to clean both the sample window on pedestal and mirror in lid arm prior to applying the next sample Note It could be helpful to apply the blank a second time and read it as a sample to ensure a proper blank 12 Apply sample to the sample window on pedestal and press the sample button to in...

Page 62: ...o Sequence or Custom see Table 1 on page 66 The miRNA sequence and Oligo sequence options allow the sequence to be entered and the extinction coefficient will be automatically calculated A minimum of 4 bases is required The default setting for Custom is 50 The extinction coefficient 1 Ɛ can be set in a range of 15 150 It is possible to enter a name for the custom nucleic acid factor for documentat...

Page 63: ... conditions are detected even if the air bubble recognition is set to off 7 For dye labelled samples tick the dye labels in the list which should be used for result calculation Note If the used dye is not available in the list please go to preferences and add a custom dye to the dye list see page 126 Dyes There is an option for dye correction which can be enabled disabled with a toggle switch Note...

Page 64: ... column 9 To confirm the parameter setting swipe the screen to the left and the sample map screen is shown The sample map offers the possibility to select cells and define sample IDs for measurements It is not necessary to make any selection If cells are left empty without defined sample IDs for horizontal or vertical sample loading all 12 positions for horizontal loading or all 8 positions for ve...

Page 65: ... corner or swipe from the bottom edge in the screen 11 Use a lint free laboratory wipe to clean both the sample windows on pedestal and mirrors in lid arm prior to applying the next sample Note It could be helpful to apply the blank a second time and read it as a sample to ensure a proper blank 12 Apply samples to the illuminated sample windows on pedestal and press the sample button to initiate t...

Page 66: ... of constituent nucleotides entered Oligo Seq calculated via extinction coefficient of constituent nucleotides entered Custom Option to enter any factor between 15 and 150 ng cm µl Dye labeled Nucleic Acid Concentration For dye labeled nucleic acids the concentration of the nucleic acid is calculated using a modified form of the Beer Lambert equation For these calculations the instrument considers...

Page 67: ... the dye the dye specific extinction coefficient see Table 2 on page 68 The dye concentration is calculated with or without background correction as follows With background correction C Amax dye ABKG Ð εdye 10 6 Without background correction C Amax dye Đ εdye 10 6 C Concentration in ng µl Amax dye Absorbance value at the absorbance maximum of the dye 10 mm path ABKG Absorbance at 320 nm 10 mm path...

Page 68: ... dependent correction factor at 260 nm Table 2 Dye Types Absorbance Max Extinction coefficient and dye dependent correction factors NanoPhotometer Model Dye Type Absorbance maximum of Dye nm Dye dependent ext coeff εdye in M 1 cm 1 Dye dependent correction factor at 260 nm cfdye NP80 N60 N50 C40 Alexa Fluor 350 345 18 400 0 25 NP80 N60 N50 C40 Alexa Fluor 488 492 62 000 0 30 NP80 N60 N50 C40 Alexa...

Page 69: ...rbance at 230 nm can indicate the presence of impurities as well 230 nm is near the absorbance maximum of peptide bonds and also indicates buffer contamination since TRIS EDTA and other buffer salts absorb at 230nm When measuring RNA samples the 260 230 ratio should be 2 0 a ratio lower than this is generally indicative of contamination with guanidinium thiocyanate a reagent commonly used in RNA p...

Page 70: ...y performing calculations based on specific ε values either pre programmed in the instrument or entered manually by the user Extinction coefficient ε values at 280 nm vary greatly for different proteins due to their particular aromatic amino acid content Fixed ε values are pre programmed in the software for certain proteins see Table 3 on page 79 However if the protein of interest is not included ...

Page 71: ... Human IgE Human Lysozyme OD1 Custom Molar Extinction Coefficient Custom Extinction Coefficient and Custom 1 Ɛ For Custom Mol Ext Coefficient enter Molecular Weight in g mol and the Mol Ext Coefficient in M 1 cm 1 For Custom Ext Coefficient enter the Ext Coefficient in l g cm For Custom 1 Ɛ enter the calculated protein factor 1 Ɛ 4 For NanoVolume application Select the volume of sample to be appli...

Page 72: ...ion ranges of different lids see page 52 Submicroliter Cell Measurements Basics Required sample volume for each lid is shown Note A new Blank is recommended when changing the dilution lid 5 The wavelength for protein measurements can be changed in the range of 200 330nm depending on the wavelength peak of the protein Default setting is 280 nm 6 Select the Units in which the concentration should be...

Page 73: ... bubble recognition is set to off 9 For dye labelled samples tick the dye labels in the list which should be used for result calculation Note If the used dye is not available in the list please go to preferences and add a custom dye to the dye list There is an option for dye correction which can be enabled disabled with a toggle switch Note Dye correction is only available for single dye selection...

Page 74: ...ad it as a sample to ensure a proper blank 13 Apply sample to the sample window on pedestal and press the sample button to initiate the measurement If auto sample is active the sample measurement is automatically started after closing the lid arm Note Auto sample function is only available NanoVolume methods and new NanoPhotometer versions it may not be available after updating an older firmware v...

Page 75: ...om 1 Ɛ enter the calculated protein factor 1 Ɛ 4 The wavelength for protein measurements can be changed in the range of 200 330nm depending on the wavelength peak of the protein Default setting is 280 nm 5 Select the Units in which the concentration should be calculated Options are ng µl µg µl µg ml and mg ml default 6 The background correction is enabled at 320 nm by default It is possible to dis...

Page 76: ... dye labelled samples tick the dye labels in the list which should be used for result calculation Note If the used dye is not available in the list please go to preferences and add a custom dye to the dye list There is an option for dye correction which can be enabled disabled with a toggle switch Note Dye correction is only available for single dye selection 9 Select the sample loading orientatio...

Page 77: ...rs the possibility to select cells and define sample IDs for measurements It is not necessary to make a selection If cells are left empty without defined sample IDs for horizontal and vertical sample loading all 12 positions for horizontal loading and all 8 positions for vertical loading are measured To measure a defined amount of samples select a cell pattern starting at A01 Sample IDs can be def...

Page 78: ...dge in the screen 12 Use a lint free laboratory wipe to clean both the sample windows on pedestal and mirrors in lid arm prior to applying the next sample Note It could be helpful to apply the blank a second time and read it as a sample to ensure a proper blank 13 Apply samples to the illuminated sample windows on pedestal and press the sample button to initiate the measurement If auto sample is a...

Page 79: ...SA Human 1 718 0 5820 40 370 69 365 IgG Mouse 0 714 1 4000 224 000 160 000 IgG Human 0 735 1 3600 204 000 150 000 IgE Human 0 654 1 5300 290 700 190 000 Lysozyme 0 379 2 6400 37 984 14 388 OD1 1 000 N A N A N A Dye labeled Protein UV280 Concentration For dye labeled proteins the concentration of the protein is calculated using a modified form of the Beer Lambert equation For these calculations the...

Page 80: ...4 on page 82 The dye concentration is calculated with or without background correction for are as follows With background correction C Amax dye ABKG Ð εdye 10 6 Without background correction C Amax dye Ð εdye 10 6 C Concentration Amax dye Absorbance at the max dye absorbance value 10 mm path ABKG Absorbance at selected background wavelength 10 mm path εdye Extinction coefficient of dye in M 1 cm 1...

Page 81: ...NanoPhotometer N120 NP80 N60 N50 C40 User Manual Version 4 3 1 81 ...

Page 82: ...at 280 nm cfdye NP80 N60 N50 C40 Alexa Fluor 350 346 19 000 0 19 NP80 N60 N50 C40 Alexa Fluor 405 401 34 500 0 70 NP80 N60 N50 C40 Alexa Fluor 488 494 71 000 0 11 NP80 N60 N50 C40 Alexa Fluor 532 530 81 000 0 09 NP80 N60 N50 C40 Alexa Fluor 546 554 104 000 0 12 NP80 N60 N50 C40 Alexa Fluor 555 555 150 000 0 08 NP80 N60 N50 C40 Alexa Fluor 568 577 91 300 0 46 NP80 N60 N50 C40 Alexa Fluor 594 590 73...

Page 83: ...260 280 ratios of 0 57 If a ratio is detected out of the acceptable range an alert icon is shown in the results table area A push on the alert icon shows additional information The ranges for acceptable ratio values can be defined in preferences The ratio is calculated with or without background correction according to if the background correction is activated during the measurements or not as fol...

Page 84: ...tions at 595 nm This standard is usually BSA bovine serum albumin Biuret Assay Method depends on a reaction between cupric ions and peptide bonds in an alkali solution resulting in the formation of a complex absorbing at 546 nm Note Biuret is only available for cuvette measurements NP80 and C40 BCA Assay Method depends on a reaction between cupric ions and peptide bonds coupled with the detection ...

Page 85: ...y Assay not for N50 4 For Cuvette Application Select the path length depending on the used cuvette Options are 0 5 mm 1 mm 2 mm 5 mm and 10 mm If it is desired to heat the sample to 37 C use the toggle switch to turn on cell holder heater When the cuvette holder has reached 37 C the toggle color changes to green Note Only available for cuvette applications NP80 and C40 For NanoVolume Application S...

Page 86: ...s shown Note A new Blank is recommended when changing the dilution lid 5 Default values for the baseline correction are depending on selected protein assay type and instrument version BCA default value 750 nm N50 off Biuret default off Bradford default value 750 nm N50 350 nm Lowry default value 405 nm Note It is recommended to use the default baseline for each assay 6 Select curve fit type Option...

Page 87: ...nual Version 4 3 1 87 7 Select Unit 8 Add up to 20 Concentrations by pushing on the Add Concentration button Added concentrations can be deleted with Enter the concentrations of the standards for the standard curve 9 Select Replicates none 2 or 3 ...

Page 88: ...surement If auto sample is active the sample measurement is automatically started after closing the lid arm Note Auto sample function is only available NanoVolume methods and new NanoPhotometer versions it may not be available after updating an older firmware version Note Once the sample measurement is initiated it is no longer possible to change the standard curve MEASUREMENT PROTOCOL MULTI SAMPL...

Page 89: ...ommended to use the default baseline for each assay 5 Select curve fit type Options are linear regression zero regression forces the straight line through the origin and 2nd order regression 6 Select Unit 7 Add up to 20 Concentrations by pushing on the Add Concentration button Added concentrations can be deleted with Enter the concentrations of the standards for the standard curve ...

Page 90: ... To confirm the parameter setting swipe the screen to the left and the standard map screen is shown The standard map is created automatically depending on the parameter settings sample orientation concentration and replicates It is not possible to change the Sample IDs for standards e g horizontally 5 standards and 2 replicates defined ...

Page 91: ...ements Results will be shown in the table of the standard map Once all standards are measured the screen changes automatically to the standard curve screen 12 In the graph screen the standard curve is shown and if replicates are defined the results and mean calculation of each standard It is possible to exclude single standard measurements with the toggle switches and standard curve is recalculate...

Page 92: ...s can be defined by pushing on the sample ID icon in the navigation bar The opened pop up offers options to define import export or delete sample IDs Further information about cell selection and sample ID definition can be found in chapter 3 NanoPhotometer Basics Sample Map on page 36 13 Depending on measuring or loading a standard curve a blank reading is required To initiate a blank apply the bl...

Page 93: ... push the store method button and enter a method name select a folder and save with the Store button Methods can be opened in the Stored Methods menu on the homescreen Opening a saved Protein Assay method shows a message with the option to load or remeasure the standard curve CALCULATIONS Protein concentration is determined using the standard curve by correlating absorbance values of samples with ...

Page 94: ...sts in the case of biochemical reactions are generally represented by enzymes which are specialized protein catalysts However a few examples of special reactions catalyzed by RNA molecules also exist Studying the kinetics of a reaction can reveal important details of the catalytic mechanism involved in terms of sequence steps transition state of reactants or nature of enzyme inhibitors MEASUREMENT...

Page 95: ...cs Required sample volume for each lid is listed to the right Note A new Blank is recommended when changing the dilution lid 4 Default wavelength is 340 nm but can be changed in the range of 200 900 nm N50 200 650 nm depending on the application 5 Time settings Enter the duration time in minutes over which measurements are to be taken Possible range is 1 3000 minutes Enter the interval time betwee...

Page 96: ... the parameters save data or delete data Change of parameters is only possible before starting the kinetic readings Save and delete data is only available after the kinetic session is stopped Note Auto print and cryo label print is not available in Kinetics CALCULATIONS All absorbance values are normalized to a 10 mm path A0 absorbance of start value 10 mm path An absorbance of actual value time n...

Page 97: ...slit monochromator optics slit geometry etc different spectrophotometer types will tend to give different OD 600 readings for the same turbid sample Therefore if results from different spectrophotometers are to be compared they must be normalized first using appropriate calibration curves For more information see Technical Note 8 OD 600 which can be downloaded on the Implen webpage www implen de s...

Page 98: ...ength depending on the used cuvette Options are 0 5 mm 1 mm 2 mm 5 mm and 10 mm If it is desired to heat the sample to 37 C use the toggle switch to turn on cell holder heater When the cuvette holder has reached 37 C the toggle color changes to green Note Only available for cuvette applications NP80 and C40 For NanoVolume Application Select the dilution depending on the sample concentration Note T...

Page 99: ...he dilution lid 4 Default wavelength is 600 nm but the wavelength can be changed in the range of 200 900 nm N50 200 650 nm depending on the application 5 Toggle switch cells ml is disabled by default Enable cells ml to get the cells ml calculated Enter the cell specific factor and multiplier e g 1 OD600 5 x 108 cells ml 6 Enter the correction factor to compensate for different optical configuratio...

Page 100: ... measurement 10 Insert cuvette with the sample and select the sample button to initiate the measurement CALCULATIONS OD600 A600 Ð cf OD600 Optical density at 600 nm A600 Absorbance at 600 nm 10 mm path Ð Dilution factor cf Correction factor for spectrophotometer Cells ml A600 Ð cf multiplier A600 Absorbance at 600 nm 10 mm path Ð Dilution factor cf Correction factor for spectrophotometer multiplie...

Page 101: ...is possible to add up to 20 different wavelengths The wavelength method includes a calculation tool to define and calculate customer defined formulas MEASUREMENT PROTOCOL 1 Select the More Apps icon from the home screen and the Wavelength icon from the More Apps screen 2 To change between NanoVolume and Cuvette application use the Change to Cuvette NanoVolume button below the parameter area NP80 C...

Page 102: ...ifferent lids see page 52 Submicroliter Cell Measurements Basics Required sample volume for each lid is shown Note A new Blank is recommended when changing the dilution lid 4 Enter desired wavelength to be measured It is possible to measure up to 20 wavelengths simultaneously More wavelength options can be added by selecting the Add Wavelength button Added wavelength can be deleted with 5 Baseline...

Page 103: ...significant figures use period for the decimal separator and the following numeric operations add subtract multiply devide and brackets To add absorbance results to the formula use the following format AXXX e g for absorbance at 260 nm A260 8 Option to set calculate a dilution factor for manual diluted samples 9 Apply the blank ddH20 or buffer to the illuminated sample window on pedestal for the r...

Page 104: ...Photometer versions it may not be available after updating an older firmware version CALCULATIONS Formula Calculation Is depending on the entered formula in the parameter concentration Absorbance Calculation Absorbance is formally defined as the decimal logarithm base 10 of the reciprocal of transmittance A log 1 T log T T 10 A Note Corresponding absorbance value e g Absorbance value 230 etc norma...

Page 105: ...n depending on the sample concentration Note There is no automatic path length setting in this method Select either a virtual dilution of 15 path length 0 67 mm or of 140 path length 0 07 mm For cuvette application Select the path length depending on the used cuvette Options are 0 5 mm 1 mm 2 mm 5 mm and 10 mm If it is desired to heat the sample to 37 C use the toggle switch to turn on cell holder...

Page 106: ...full scan range of 200 900 nm N50 200 650 nm 5 Baseline correction is set off by default Enabling the baseline corrections shows a list with different wavelength options 377 nm 604 nm 650 nm 770 nm N A N50 and 823 nm N A N50 Option to enter any wavelength between 200 nm and 900 nm N50 650 nm 6 Option to smooth the graph with different boxcars Options Off 1 boxcar 11 default 2 boxcar 21 and 3 boxca...

Page 107: ...n pedestal and press the sample button to initiate the measurement If auto sample is active the sample measurement is automatically started after closing the lid arm Note Auto sample function is only available NanoVolume methods and new NanoPhotometer versions it may not be available after updating an older firmware version MEASUREMENT PROTOCOL MULTI SAMPLE 1 Select the More Apps icon from the Hom...

Page 108: ...ections shows a list with different wavelength options 377 nm 604 nm 650 nm 770 nm and 823 nm Option to enter any wavelength between 200 nm and 900 nm 6 Option to smooth the graph with different boxcars Options Off 1 boxcar 11 default 2 boxcar 21 and 3 boxcar 61 Select the sample loading orientation There are two options Horizontally Measure up to 12 samples A01 A12 shown in sample map as row ...

Page 109: ...define sample IDs for measurements It is not necessary to make a selection If cells are left empty without defined sample IDs for horizontal and vertical sample loading all 12 positions for horizontal loading and all 8 positions for vertical loading are measured To measure a defined amount of samples select a cell pattern starting at A01 Sample IDs can be defined by pushing on the sample ID icon i...

Page 110: ...next sample Note It can be helpful to apply the blank a second time and read it as a sample to ensure a proper blank 10 Apply samples to the illuminated sample windows on pedestal and press the sample button to initiate the measurement If auto sample is active the sample measurement is automatically started after closing the lid arm Note Auto sample function is only available NanoVolume methods an...

Page 111: ...ct the dilution depending on the sample concentration Note There is no automatic path length setting in this method Select either a virtual dilution of 15 path length 0 67 mm or of 140 path length 0 07 mm For cuvette application Select the path length depending on the used cuvette Options are 0 5 mm 1 mm 2 mm 5 mm and 10 mm If it is desired to heat the sample to 37 C use the toggle switch to turn ...

Page 112: ...e added by selecting the Add Ratio button Added ratios can be deleted with 5 Baseline correction is set off by default Enabling the baseline corrections shows a list with different wavelength options 377 nm 604 nm 650 nm 770 nm N A N50 and 823 nm N A N50 Option to enter any wavelength between 200 nm and 900 nm N50 650 nm 6 Option to smooth the graph with different boxcars Options Off 1 boxcar 11 d...

Page 113: ... apply the blank a second time and read it as a sample to ensure a proper blank 10 Apply sample to the sample window on pedestal and press the sample button to initiate the measurement Note Auto sample function is only available NanoVolume methods and new NanoPhotometer versions it may not be available after updating an older firmware version CALCULATIONS The absorbance ratio is calculated from th...

Page 114: ... To change between NanoVolume and Cuvette application use the Change to Cuvette NanoVolume button below the parameter area NP80 C40 only 3 For NanoVolume application Select the dilution depending on the sample concentration Note There is no automatic path length setting in this method Select either a virtual dilution of 15 path length 0 67 mm or of 140 path length 0 07 mm For cuvette application S...

Page 115: ...efault wavelength is 260 nm but can be changed in the range of 200 900 nm N50 200 650 nm depending on the sample application 5 Enter a factor for concentration calculation 6 Unit selection 7 Baseline correction is set off by default Enabling the baseline corrections shows a list with different wavelength options 377 nm 604 nm 650 nm 770 nm N A N50 and 823 nm N A N50 Option to enter any wavelength ...

Page 116: ...11 default 2 boxcar 21 and 3 boxcar 61 9 Option to set calculate a dilution factor for manual diluted samples 10 Apply the blank ddH20 or buffer to the illuminated sample window on pedestal for the reference measurement and select blank to initiate the reading Note The illumination of the sample window can be switched off in the preferences ...

Page 117: ... is automatically started after closing the lid arm Note Auto sample function is only available NanoVolume methods and new NanoPhotometer versions it may not be available after updating an older firmware version CALCULATIONS In this method the concentration of the sample is calculated based on the Beer Lambert law given the user specified wavelength of interest and user defined extinction coeffici...

Page 118: ...or concentration MEASUREMENT PROTOCOL 1 Select the More Apps icon from the home screen and the Standard Curve icon from the More Apps screen 2 To change between NanoVolume and Cuvette application use the Change to Cuvette NanoVolume button below the parameter area NP80 C40 only 3 For NanoVolume application Select the dilution depending on the sample concentration Note There is no automatic path le...

Page 119: ...A new Blank is recommended when changing the dilution lid 4 Baseline correction is set off by default Enabling the baseline corrections shows a list with different wavelength options 377 nm 604 nm 650 nm 770 nm N A N50 and 823 nm N A N50 Option to enter any wavelength between 200 nm and 900 nm N50 650 nm 5 Select the curve fit type Options are linear regression zero regression forces the straight ...

Page 120: ...ng on the Add Concentration button Added concentrations can be deleted with Enter the concentrations of the standard curve 8 Select Replicates none 2 or 3 9 Once Standard curve is created or loaded it will be used for concentration calculations in the method It might be necessary to do a blank measurement ...

Page 121: ...re version Note Once the sample measurement is initiated it is not possible to make changes to the standard curve CALCULATIONS Concentration is determined via the absorbance values provided by the standard curve based on the curve fit selection including the following options linear regression zero regression and 2nd order regression CUSTOM APPS There is an option for designing customer specific C...

Page 122: ...he file path of the selected folder is shown on the top of the right file area Note PDF and Excel files cannot be opened on the NanoPhotometer Files need to be transferred to a computer or device where Excel or a PDF reader is installed Note Control device is only available on computer tablets and smartphones not on the NanoPhotometer version of the software For data transfer via Ethernet or WiFi ...

Page 123: ...ability Pushing a storage folder shows the subfolders of this method folder on the left side and the individual files on the right side On the right side of the screen all saved method files of the selected folder are shown and can be opened by a long or double click On the top of the right area the file path of the selected folder is shown New folders can be created by pushing on Folders can be d...

Page 124: ...eral in the Preferences menu opens a window to the right of the preferences menu with the following options Date and Time Display About Storage Language and Illumination Sample Window N120 NP80 N60 N50 or Enable NanoVolume C40 DATE AND TIME Within the Date and Time it is possible to set the actual date and time of the NanoPhotometer or change the time zone To change the time zone or the date and o...

Page 125: ...ver off Note The screensaver option is not available when the CFR21 software is enabled ABOUT In About the following information of the NanoPhotometer are shown NanoPhotometer Version Serial Number Ethernet IP Address WiFi IP Address Hardware Version Firmware Version Time Date of Initialization Test and Status of Initialization STORAGE Shows the total storage capacity and the free space of the int...

Page 126: ... printouts will be in English Independent from the language setting utilized in the method ILLUMINATION SAMPLE WINDOW Toggle switch to switch on off the illumination of the sample window for N120 NP80 N60 N50 only Note Only available for N120 NP80 N60 N50 models ENABLE NANOVOLUME Toggle switch to enable the submicroliter cell option for the NanoPhotometer C40 Note Only available for C40 model DYES...

Page 127: ...nd dye dependent correction factor as well as the option to show the dye in the parameter list of the application Nucleic Acid or Protein UV Note It is not possible to delete a dye from the default factory list custom dyes can be deleted if they are not locked It is possible to add a new dye to the list by selecting the button to add a new dye A window will open where it is possible to enter the d...

Page 128: ...otometer Note Blank Control is available for all NanoVolume methods for N120 NP80 N60 N50 SAMPLE QUALITY CONTROL It is possible to change the upper and the lower limit of the ratio alert warning messages Default values for nucleic acid ratios are 260 230 ratio 1 8 A 3 A and 260 280 ratio 1 65 A 2 5 A Default value for Protein UV ratio is 260 280 ratio is 0 7 A ...

Page 129: ...erver Access and Network Folder NETWORK SETTINGS In Network Settings it is possible to choose between a Dynamic Host Configuration Protocol DHCP and a static network configuration The DHCP protocol is used by default Connect the NanoPhotometer with the Ethernet and the IP is automatically set during the startup of the NanoPhotometer If no IP Address is listed for Current IPv4 push the disk icon to...

Page 130: ...to set up a WiFi Hotspot WiFi Access Point or a WiFi network To set up a WiFi Hotspot select Enable NanoPhotometer Hotspot in the dropdown and push on the save icon The WiFi Hotspot can be used to connect a computer Android smartphone tablet iPad or iPhone via WiFi to the NanoPhotometer To set up a WiFi network select Connect to WiFi Network in the dropdown Select an available WiFi network from th...

Page 131: ...NanoPhotometer file server via network By default access to the file server is open to any user It is necessary to create at least one user account to activate the file server access option To create a user account push on the button and enter a user name and password Confirm with the disk icon Note Allowed characters for user name are A Z a z 0 9 _ Do not use Blank character ...

Page 132: ...der for saving data directly from the NanoPhotometer to a network folder To add a network folder push the icon and enter network folder nickname and network path of the network folder If the local network requires authentication enter user name and password for login and if necessary the domain To save the Network folder push the disk icon ...

Page 133: ...and some printer configuration settings might need to be changed in order to allow communication with the NanoPhotometer Enter the IP of the network printer to the input window Note For printing on network printer the NanoPhotometer needs to be connected to the network either via Ethernet LAN cable or WiFi network Note If a printer is directly connected to the NanoPhotometer via USB this printer w...

Page 134: ...n the Nucleic Acid Protein buttons in the header CFR21 The CFR21 Software is an optional software tool ideal for GxP laboratories which require proper electronic record keeping It includes user management access control electronic signatures data integrity security and audit trail functionality In the CFR21 Preferences it is possible to enable the optional CFR21 Software This is only possible with...

Page 135: ...on and has detected either an air bubble lint residue or a bad sample like e g a turbid sample Check sample clean the sample window on pedestal and mirror in the lid arm thoroughly and reapply sample carefully To avoid air bubbles apply sample by reverse pipetting Another control device is currently connected Message is shown on a control device when another control device is currently connected w...

Page 136: ...u really want to intercept This message is shown on the NanoPhotometer when a method is open on another control device Push on OK and the measurements session is interrupted and the data are saved in the auto save folder Lint residue or bad sample Please reapply sample Displayed when Air Bubble Recognition is off Software has detected either a lint residue or a bad sample like e g a turbid sample ...

Page 137: ...ersion than the installed one NPOS software is not downward compatible Firmware not found NPOSX bin must be in root folder of USB flash drive NPOSX bin file must be in the root folder of the USB flash drive If necessary unzip the downloaded installation file The file name should not have any additions or extensions like from multiple download Do NOT rename a NPOS bin to NPOSX bin Make sure that th...

Page 138: ...on problem during an open measurement session Reboot the NanoPhotometer If the message still appears please create a log file and send it to Implen Customer Support Software update failed Please contact Implen customer support Make sure that the sample name is NPOS bin without any additions or extensions like from multiple downloads otherwise rename file to be NPOS bin exactly NPOS bin file must b...

Page 139: ...on to report a problem is only available for computer and tablet versions Selection of Report Problem in the assistance menu a form with the following information is shown on the right side first name last name phone number email and country A dropdown menu provides the option to select the type of problem and includes the following choices error message software firmware measurements and other It...

Page 140: ...e all data on the NanoPhotometer before updating Note Make sure that the NanoPhotometer is connected to power and that the power connection is not interrupted during the update It is recommended to do the update via the built in touchscreen If the touchscreen is not available it is also possible to do the update via computer or tablet In this case it is necessary to relaunch the NanoPhotometer sof...

Page 141: ...window will open that says Reset the NanoPhotometer Selecting the cancel button will close the window without changing the settings and selecting reset will open a window that will ask again Reset the NanoPhotometer to factory settings All data stored methods and settings will be lost If it is confirmed the factory settings will be restored Note All stored methods settings and data on the NanoPhot...

Page 142: ...plen support support implen de REMOTE ACCESS Option to enable remote acces ping and Rest API Push Services Remote access and ping are active after booting the NanoPhotometer and are automatically switched off after 15 minutes A renewed activation for remote access is possible for 30 minutes at a time The activation of ping remains active until the next restart of the NanoPhotometer Rest API push s...

Page 143: ...s The NPOS software includes some open source software components under the licenses listed END USER LICENSE AGREEMENT EULA Shows the end user license agreement TRADEMARKS Windows is a trademark of Microsoft Airprint Mac OS OS X iOS iPhone and iPad are trademarks of Apple Android OS is a trademark of Google Linux is a trademark of Linus Torvalds ...

Page 144: ...lease contact the Implen team directly Europe Asia South Pacific Middle East and Africa Implen GmbH Schatzbogen 52 81829 München Germany Phone 49 89 72637180 Fax 49 89 726371854 Email support implen de www implen de North and South America Implen Inc Unit 104 31194 La Baya Drive Westlake Village CA 91362 USA Phone 1 818 748 6400 Telefax 1 818 449 0416 Email support implen com Website www implen co...

Page 145: ...idelines concerning GLP GMP include calibration certification using filters traceable to international standards during production and quality control certified engineers and calibrated test equipment approved to ISO 9001 standard automatic self diagnostic calibration test during start of the NanoPhotometer result is documented in each data output file and possibility to save a Implen Document Sou...

Page 146: ...NanoPhotometer and disconnect the power cord prior to external cleaning Use a soft wet cloth or dry microfiber cloth to clean all external surfaces A mild liquid detergent may be used to remove stubborn marks Approved disinfectant solutions include Apesin disinfection spray Tana Chemi GmbH Incidin Liquid Inciddin Foam Ecolab and Lysoformin Spezial Lysoform Dr Hans Roseman GmbH Note Observe all nec...

Page 147: ...oughly tested to ensure that it meets its published specification The warranty is as defined in our current terms and conditions and is only valid if the product has been used according to the instructions supplied Implen or your supplier can accept no liability of loss or damage arising from the faulty or incorrect use of this product ...

Page 148: ...protože se může zdeformovat nebo explodovat baterie Neskladujte ani nepoužívejte přístroj v blízkosti jakéhokoli tepelného zdroje především při teplotách nad 60 C nebo ve výbušné atmosféře Nenechávejte přístroj NanoPhotometer na klíně nebo jakékoli části těla zabráníte tak nepohodlí nebo poranění vlivem působení žáru Nepokládejte na přístroj NanoPhotometer žádné předměty Přístroj NanoPhotometer se...

Page 149: ...o přístroje Zajistěte aby po zapnutí přístroje žádné materiály nebránily cirkulaci vzduchu Vyhněte se přímému slunečnímu záření protože může docházet k vyblednutí částí přístroje a poškození plastových dílů Přístroj byste měli umístit tak aby v případě nouze bylo možné snadno vyhledat a odpojit hlavní přívod Přístroj přenášejte vždy tak že jej budete držet za hlavní část a nikoli např za volitelný...

Page 150: ...che elektrische velden water chemische stoffen of andere vloeistoffen zoals zware regen of vocht Plaats het instrument niet in vuur Dit kan opzwellen of ontploffen batterij Gebruik niet op of nabij een warmtebron in het bijzonder temperaturen boven 60 C of in een explosieve omgeving Plaats uw NanoPhotometer niet op uw schoot of een ander lichaamsdeel om ongemak of letsel door warmte te voorkomen P...

Page 151: ...bereikt 2 tot 3 uur Maximale relatieve luchtvochtigheid niet condenserend 80 en tot 31 C lineair afnemend tot 50 bij 40 C Het instrument moet op een stabiel vlak oppervlak worden geplaatst dat 4 5 kg kan dragen en moet zo worden geplaatst dat lucht vrij rond het instrument kan stromen Zorg ervoor dat de luchtcirculatie niet wordt geblokkeerd wanneer het instrument is ingeschakeld Voorkom rechtstre...

Page 152: ...technische problemen ervaart of problemen merkt met het verwerken van monsters Opmerking Indien deze apparatuur wordt gebruikt op een andere manier dan hierboven vermeld of in een omgeving die niet geschikt is voor veilig gebruik kan de voorziene bescherming van de apparatuur beschadigd raken en vervalt de garantie van het instrument ...

Page 153: ... avec votre NanoPhotometer N utilisez que le bloc d alimentation chargeur fourni N exposez pas le NanoPhotometer à de puissants champs magnétiques électriques à de l eau à des produits chimiques ou à d autres types de liquide par exemple à de fortes précipitations ou à de l humidité N exposez pas l instrument au feu car il pourrait gonfler ou exploser batterie Ne stockez pas et n utilisez pas l in...

Page 154: ...ison Si des éléments sont manquants informez en immédiatement votre fournisseur Inspectez l instrument pour vérifier qu il ne présente pas de signes d endommagement liés au transport Si vous constatez la présence de tels signes d endommagement informez en immédiatement votre fournisseur Assurez vous que le site d installation que vous avez prévu est conforme aux conditions environnementales nécess...

Page 155: ...mière utilisation Pour éviter une décharge profonde de la batterie chargez la batterie au moins une fois par mois même si le NanoPhotometer n est pas utilisé Il n est pas possible de recharger les batteries profondément déchargées elles doivent être remplacées par des batteries neuves Veuillez lire le manuel d utilisation complet avant la première utilisation Mettez l équipement sous tension à l a...

Page 156: ...explodieren kann Nicht in der Nähe von Wärmequellen aufstellen bzw verwenden insbesondere keinen Temperaturen über 60 C aussetzten Betreiben sie das NanoPhotometer nicht auf Ihrem Schoß oder in der Nähe eines Körperteils um Unannehmlichkeiten oder Verletzungen durch Hitzeeinwirkung zu verhindern Stellen Sie keine Gegenstände auf dem NanoPhotometer ab NanoPhotometer mit Akku Pack mobile Version müs...

Page 157: ...das Gerät für ca 2 3 Stunden aus und nach Erreichen der Raumtemperatur wieder ein Maximale relative Luftfeuchtigkeit nicht kondensierend von 80 und bis zu 31 C bei einer abnehmenden Linearität von 50 bei 40 C Das Gerät muss auf einer stabilen ebenen Fläche aufgestellt werden die für 4 5 kg ausgelegt ist Weiterhin sollte die Luft frei um das Gerät zirkulieren können Stellen Sie sicher dass während ...

Page 158: ...urch Bitte lesen Sie die vollständige Bedienungsanleitung vor dem Gebrauch Bitte kontaktieren Sie die ursprünglichen Lieferanten sofort falls technische oder Handhabungs Probleme auftreten Hinweis Wenn dieses Gerät in einer Art und Weise verwendet wird die nicht spezifiziert ist oder in für einen sicheren Betrieb nicht geeigneten Umgebungsbedingungen können die Schutzfunktionen des Geräts beeinträ...

Page 159: ... a forti campi elettrici e o magnetici acqua sostanze chimiche o qualsiasi tipo di liquido ad esempio pioggia fitta o umidità Non porre lo strumento sul fuoco poiché può gonfiarsi o esplodere la batteria Non conservarlo e non utilizzarlo in prossimità di fonti di calore specificamente a temperature maggiori di 60 C o in un atmosfera esplosiva Il NanoPhotometer non deve essere poggiato sulle gambe ...

Page 160: ...necessario attendere che si stabilizzi Spegnere e riaccendere lo strumento una volta che ha raggiunto la stabilità termica 2 3 ore Umidità relativa massima non condensante dell 80 e fino a 31 C con riduzione fino al 50 a 40 C Lo strumento va collocato su una superficie stabile e orizzontale in grado di sostenere un peso di 4 5 kg e tale da consentire la libera circolazione dell aria intorno allo s...

Page 161: ... Lo strumento esegue una serie di test di autodiagnostica In caso di difficoltà di natura tecnica o con la manipolazione dei campioni contattare immediatamente il fornitore originale Nota se questa apparecchiatura viene utilizzata secondo modalità diverse da quelle specificate oppure in condizioni ambientali non idonee a un utilizzo sicuro la protezione di cui è dotata potrebbe risultare compromes...

Page 162: ...ecyfikacją zasilacza lub ładowarki Nie wolno narażać urządzenia NanoPhotometer na działanie mocnych pól magnetycznych i elektrycznych wody chemikaliów ani jakichkolwiek płynów takich jak mocne opady atmosferyczne lub wilgoć Urządzenia nie wolno wrzucać do ognia ponieważ może napęcznieć lub eksplodować akumulator Urządzenia nie wolno przechowywać ani używać w pobliżu jakichkolwiek źródeł ciepła szc...

Page 163: ...ywania wynosi 0 40 C Nie przechowuj urządzenia poniżej lub powyżej tej temperatury W przypadku ekstremalnych zmian temperatury może być konieczne zrównoważenie temperatury urządzenia W tym celu należy je wyłączyć i włączyć ponownie po osiągnięciu równowagi termicznej po około 2 3 godzinach Maksymalna wilgotność względna bez kondensacji wynosi 80 w temperaturze do 31 C i zmniejsza się liniowo do 50...

Page 164: ...dzenia ze źródłem zasilania należy je włączyć przyciskiem zasilania na panelu tylnym Urządzenie wykona serię testów diagnostycznych W przypadku wystąpienia problemów technicznych lub z próbką należy niezwłocznie skontaktować się z pierwotnym dostawcą urządzenia Uwaga Eksploatacja urządzenia w sposób niezgodny ze specyfikacją lub w warunkach niesprzyjających bezpiecznemu działaniu może zmniejszyć s...

Page 165: ...tricos intensos ni tampoco a agua productos químicos o ningún otro tipo de líquido como lluvia o humedad elevada No arroje el instrumento al fuego pues podría inflamarse o explotar la batería No lo guarde ni lo utilice en las proximidades de fuentes de calor en especial a temperaturas superiores a 60 C o en una atmósfera explosiva No se apoye el NanoPhotometer en el regazo ni cerca de ninguna part...

Page 166: ...dos durante el transporte Si se observan daños informe de inmediato a su distribuidor Asegúrese de que el lugar donde desea instalar la unidad cumple las condiciones ambientales sobre funcionamiento seguro uso en interiores o en un ambiente seco Nota No coloque el NanoPhotometer cerca de líquidos o productos químicos ni en entornos con lluvia humedad o polvo Rango de temperatura 10 40 C NP80 N60 N...

Page 167: ...meter no está en uso No es posible recargar las baterías completamente descargadas ya que deben ser sustituidas por otras nuevas Por favor lea el manual de usuario completo antes de la primera utilización Encienda el instrumento con el botón de encendido situado en el panel posterior una vez enchufado El instrumento llevará a cabo una serie de pruebas de autodiagnóstico Contacte con el distribuido...

Page 168: ...ätska som kraftigt regn eller fukt Lägg inte instrumentet i eld det kan då svälla och explodera batteriet Förvara inte och använd inte heller i närheten av någon typ av värmekälla särskilt inte temperaturer över 60 C eller i en explosiv atmosfär För att förhindra obehag av eller skada från värmeexponering ska din NanoPhotometer inte läggas i ditt knä eller i närheten av någon kroppsdel Placera int...

Page 169: ...1 C linjärt minskande till 50 vid 40 C Instrumentet måste placeras på en stabil jämn yta som kan bära 4 5 kg och tillåter att luft fritt kan cirkulera runt instrumentet Kontrollera när den är påslagen att inget material minskar luftcirkulationen Undvik direkt solljus eftersom det kan bleka delar av instrumentet och orsaka skada på delar som är tillverkade av plast Utrustningen ska placeras på så s...

Page 170: ...ual Version 4 3 1 170 Obs Om den här utrustningen används på ett sätt som inte är föreskrivet eller i miljöförhållanden som inte är lämpliga för säker drift kan skyddet som utrustningen tillhandahåller försämras och instrumentets garanti förverkas ...

Page 171: ...elétricas excessivas água produtos químicos ou qualquer outro tipo de líquido como a chuva forte ou a humidade Não submeta o instrumento a fogo dado que pode dilatar ou explodir bateria Não guarde nem use o instrumento na proximidade de qualquer tipo de fonte de calor especialmente a temperaturas superiores a 60 C ou em ambientes explosivos Não use o NanoPhotometer sobre o seu colo ou regaço nem j...

Page 172: ... for submetido a alterações extremas de temperatura pode ser necessário permitir o respetivo equilíbrio Desligue o instrumento e volte a ligar depois de alcançar o equilíbrio térmico 2 3 horas A humidade relativa máxima não condensação é de 80 e até 31 C reduzindo progressivamente até 50 a 40 C O instrumento deve ficar posicionado numa superfície nivelada e estável e que suporte 4 5 kg e que permi...

Page 173: ...te O instrumento realiza um conjunto de verificações em auto diagnóstico Contacte imediatamente o fornecedor original se ocorrerem dificuldades técnicas ou no manuseamento de amostras Nota Se este equipamento for utilizado de forma não especificada ou em condições ambientais não adequadas para o funcionamento em segurança a proteção proporcionada pelo equipamento pode ficar comprometida e a garant...

Page 174: ...adford Assay 83 Buttons 33 C CFR21 Software 133 Cleaning 145 Compatibility 26 Solvent 49 55 Concentration 113 Calculations 116 Measurement Protocol 113 Connecting Cable 16 Connectivity HDMI 21 LAN 20 USB 20 WiFi 20 Contact 143 Cryo Label 18 43 Custom Apps 120 Cuvette 51 D Data Transfer 56 Date and Time 123 Delete Data 46 Diagnostics 141 Didymium Glass Filter 16 DiluCell 17 Display 124 DYMO Printer...

Page 175: ...er 132 NPOS 26 Nucleic Acids 58 Calculations 65 Measurement Protocol Multi Channel 62 Measurement Protocol Single Channel 58 O OD600 96 Calculations 99 Measurement Protocol 97 On Off Switch 10 11 12 13 14 15 P Parameter area 34 PDF 45 Positioning 25 Collocazione 160 Instalación 166 Plaatsen 150 Placering 169 Platzieren 157 Posicionamento 172 Positionnement 153 Umiejscowienie 163 Umístění 148 Power...

Page 176: ...1 Serial Number 15 Side Tab Bar 34 40 Software Backup 140 Reset 140 Update 139 Solvent Compatibility 49 55 Specifications 22 Standard Curve 117 Calculations 120 Load 92 Measurement Protocol 117 Remeasure 92 Standard Solution 17 Stored Methods 47 122 Stored Results 121 Submicroliter Cell 17 33 52 System Freeze 137 T Table area 34 Technical Specifications 22 Touch Screen 145 Trademarks 142 Troublesh...

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