NanoPhotometer
®
N120/
NP80/N60/N50/C40
User Manual Version 4.3.1
49
Note:
Make sure that the metal contact face (around the measurement window and the
mirror) is clean.
Note:
Do not use aggressive solvents such as strong acids or bases or organic solvents
at any time (see page 55 Solvent Compatibility (N50/N60/N120/NP80/C40 with
submicroliter cell). If unsure please contact
about your specific reagent/solvent.
7.
It is possible to enter a sample name for each sample in the input window “enter sample
name”.
Note
: Allowed characters are: A…Z a…z 0…9 , . - ( ) @ ! = _ ~ ; [ ] { } ‘
Note:
Blank character is not allowed.
8. Raise the lid arm and pipette the appropriate amount of sample solution onto the
illuminated sample window. Upon completion of measurement raise lid arm, clean the
surfaces and apply the next sample.
Note:
Parameter setting Volume 1 - 2 µl adjusts the path length automatically. The
parameter setting Volume 0.3 µl measures only the 0.07 mm path length for higher
concentrations (dsDNA > 420 ng/µl / BSA > 12.6 mg/ml).
Note
: The sample window on pedestal must be clean and residual fluff from any cleaning
wipe must be removed for optimum performance.
N
ANO
V
OLUME
M
ULTI
S
AMPLE
M
EASUREMENT
B
ASICS
(N120)
Note
: The multi sample mode is available for the following methods: Nucleic Acid, Protein
UV, Protein Assay and Wavescan.
1. Select a multi-channel method depending on your sample and set the parameters for
the measurement.
2. Sample Map: Option to set sample IDs via the sample ID pop up ( ) or import sample
IDs with customized sample names (see page 36 Sample Map / Definition of Sample
IDs for further details).
It is not necessary to define sample IDs in the sample map for measurements.
Note
: Allowed characters for Sample IDs
are: A…Z a…z 0…9 , . - ( ) @ ! = _ ~ ; [ ] { } ‘
Blank character is not allowed.
3. Ensure that the sample spots on pedestal and the mirrors in the lid arm are clean.