NanoPhotometer
®
N120/
NP80/N60/N50/C40
User Manual Version 4.3.1
110
Further information about cell selection and sample ID definition can be found in chapter 3
NanoPhotometer
Basics / Sample Map on page 36.
8. Apply the blank ddH20 or buffer to the illuminated sample windows on
pedestal for the reference measurement and select blank to initiate the
reading.
Note
: The illumination of the sample window can be switched off in the
preferences.
Note
: If the blank and sample buttons are not shown please either open
the lid arm, push on
on the right bottom corner or swipe from the
bottom edge in the screen.
9. Use a lint-free laboratory wipe to clean both the sample windows on
pedestal and mirrors in lid arm prior to applying the next sample.
Note:
It can be helpful to apply the blank a second time and read it as a
sample to ensure a proper blank.
10. Apply samples to the illuminated sample windows on pedestal and press
the sample button to initiate the measurement.
If auto sample is active the sample measurement is automatically started
after closing the lid arm.
Note
: Auto sample function is only available NanoVolume methods and
new NanoPhotometer
®
versions it may not be available after updating an
older firmware version.
C
ALCULATIONS
No calculations necessary: values are reported based on 10 mm path length.
The results show prominent peaks with wavelength and absorbance values. For cuvette
measurements it is possible to change to the %Transmittance mode. If a peak of interest is
not shown in the results the peak can be selected by pushing on the graph. The peak can
then be added to the results by tapping on the Add Peak button in the pop-up window.