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GE AKTA ready, Operating Instructions Manual

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GE AKTA ready Operating Instructions Manual Download Page 170

11 Run a method

About this chapter

Running a method on ÄKTA ready is a largely automatic procedure. User interaction is
mainly required in the preparation phase, which includes connecting the column and
the required containers, and checking that all connections are correctly assigned and
that flow kit type and volumes are correct.

Precautions

WARNING

Before attempting to perform any of the procedures described in
this chapter, you must read and understand all contents of the
corresponding section(s) in the

Section 2.1 Safety precautions, on

page 15

as listed below:

•

General precautions, on page 15

•

Personal protection, on page 16

•

Operation, on page 19

In this chapter

This chapter contains the following sections:

See page

Section

171

11.1 Final preparations before a run

175

11.2 Starting a run

178

11.3 Viewing and printing results

180

11.4 After the run

170

ÄKTA ready Operating Instructions 28960345 AD

11 Run a method

Summary of Contents for AKTA ready

Page 1: ...ÄKTA ready Operating Instructions Original instructions ...

Page 2: ... Installation 57 4 1 Included in the delivery 58 4 2 Site requirements 60 4 3 Transport 65 4 4 Unpacking 66 4 5 Positioning the system 67 4 6 Connections 74 5 Starting ÄKTA ready 75 5 1 Start and log on to UNICORN 76 5 2 Start the system 77 6 Flow kit installation 78 6 1 ÄKTA ready flow kits 80 6 2 Unpacking the flow kit 81 6 3 Mounting the flow kit 97 7 ÄKTA ready gradient 98 7 1 ÄKTA ready gradi...

Page 3: ...2 4 Block 3 Setting PID parameters 158 10 2 5 Block 4 Filling the flow kit 160 10 2 6 Block 5 Equilibration 161 10 2 7 Block 6 Sample load 163 10 2 8 Block 7 Washing unbound sample 164 10 2 9 Block 8 Elution 166 10 2 10 Block 9 Regeneration 167 10 2 11 Saving the method 168 10 3 Creating a column efficiency test 170 11 Run a method 171 11 1 Final preparations before a run 175 11 2 Starting a run 1...

Page 4: ...214 14 5 PID control 216 14 6 Checklists 221 14 7 Health and Safety Declaration Form 223 14 8 More information 224 Index 4 ÄKTA ready Operating Instructions 28960345 AD Table of Contents ...

Page 5: ... You should have a general understanding of how a PC and Microsoft Windows works The user must have a working knowledge of UNICORN software A user account has been created according to the UNICORN Administration and Technical Manual The user must be acquainted with the use of general bioprocessing equipment and with handling of biological materials The user must read and understand Chapter 2 Safet...

Page 6: ...This chapter contains the following sections See page Section 7 1 1 Important user information 9 1 2 Regulatory information 12 1 3 Associated documentation 6 ÄKTA ready Operating Instructions 28960345 AD 1 Introduction ...

Page 7: ...nt Safety notices This user documentation contains WARNINGS CAUTIONS and NOTICES concerning the safe use of the product See definitions below Warnings WARNING WARNING indicates a hazardous situation which if not avoided could result in death or serious injury It is important not to proceed until all stated conditions are met and clearly understood Cautions CAUTION CAUTION indicates a hazardous sit...

Page 8: ...eful information that can improve or optimize your procedures Tip Typographical conventions Software items are identified in the text by bold italic text A colon separates items in a group for example Flowpath Injection valve refers to the Injection valve item in the Flowpath group Hardware items are identified in the text by bold text for example the Power button 8 ÄKTA ready Operating Instructio...

Page 9: ...rer Björkgatan 30 SE 751 84 Uppsala Sweden Conformity with EU Directives This product complies with the European directives listed in the table by fulfilling the corresponding harmonized standards A copy of the EU Declaration of Conformity is included in the documentation package Title Directive Restriction of Hazardous Substances RoHS Directive 2011 65 EU Machinery Directive MD 2006 42 EC Electro...

Page 10: ... Notes Description Standard EN ISO standard is har monized with EU direc tive 2006 42 EC Safety of machinery General principles for design Risk as sessment and risk reduction EN ISO 12100 EN standard is harmo nized with EU directive 2006 95 EC Safety requirements for electri cal equipment for measure ment control and laboratory use EN IEC 61010 1 UL61010 1 CAN CSA C22 2 No 61010 1 EN standard is h...

Page 11: ... manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be re quired to correct the interference at his own expense Environmental conformity This product conforms to the following environmental requirements Title Requirement Waste Electrical and Electronic Equipment WEEE Dir...

Page 12: ...KTA ready in a safe way this manual ÄKTA ready Operating Instructions Detailed instructions on how to use the UNICORN control system UNICORN User Manual package1 1 ÄKTA ready can be controlled by both UNICORN 5 and UNICORN 7 Third party component documentation Documentation for components produced by a third party are if existent also included in the document package 12 ÄKTA ready Operating Instru...

Page 13: ...p your ÄKTA ready operating in peak condition 18112156 Handbook Process chromatography A guide to op timization scale up and validation 28919821 Application note Efficiency test of ReadyToProcess columns 28995879 Application note Ready to use fluid management solu tions for chromatography systems 28919856 Application note Purification of a monoclonal antibody using ReadyToProcess columns ÄKTA read...

Page 14: ...ling operating or maintaining the product all users must read and understand the entire contents of this chapter to become aware of the hazards involved In this chapter This chapter contains the following sections See page Section 15 2 1 Safety precautions 24 2 2 Labels 27 2 3 Emergency procedures 30 2 4 Recycling information 31 2 5 Declaration of Hazardous Substances DoHS 14 ÄKTA ready Operating ...

Page 15: ... product and the operational processes may have on the classification of the hazardous area The process might cause the area to increase or the zone classification to change Implementtheriskreductionmeasuresneeded includ ing use of personal protective equipment The customer must make sure that all installation mainte nance operation and inspection is carried out by qualified personnel who are adeq...

Page 16: ...TICE Any computer used with the equipment must comply with IEC 60950 and be installed and used according to the manufacturer s instructions Personal protection WARNING Always use appropriate Personal Protective Equipment PPE during operation and maintenance of this product Hazardous substances and biological agents When using hazardous chemical and biological agents take all suitable protective me...

Page 17: ...one 0 to Zone 2 according to IEC 60079 10 2002 The product does not fulfill the requirements of the ATEX Directive Installing and moving WARNING Move transport crates Make sure that the lifting equipment has the capacity to safely lift the crate weight Make sure that the crate is properly balanced so that it will not accidentally tip when moved Heavy object Because of the significant weight of the...

Page 18: ... computer must be installed and used according to the instructions provided by the manufac turer of the computer CAUTION The wheels of ÄKTA ready should be locked during normal use The wheels should be unlocked only when moving the unit Make sure that all tubing hoses and cables are placed so that the risk of tripping accidents is minimized The product is designed for indoor use only Do not use th...

Page 19: ...ections are made ÄKTA ready shall be installed and prepared by personnel from GE Healthcare or third party authorized by GE Healthcare Contact GE Healthcare if you require re installation at a new site Operation WARNING Do not operate the ÄKTA ready instrument in any other way than described in the ÄKTA ready and UNICORN manuals ÄKTA ready Operating Instructions 28960345 AD 19 2 Safety instruction...

Page 20: ...ning Never put fingers or any objects other than the intended tubing in to the pinch valve opening Corrosive substance NaOH is corrosive and therefore danger ous to health When using hazardous chemicals avoid spillage and wear protective glasses and other suitable Personal Pro tective Equipment PPE Emergency stop Pressing the EMERGENCY STOP will not shut off mains power to the cabinet Power failur...

Page 21: ...erature as stated on the system la bel Check that the Pressure Control Regulator for the system air supply is set to the pressure stated in the specifications Keep UV flow cell clean Do not allow solutions containing dissolved salts proteins or other solid solutes to dry out in the flow cell Do not allow particles to enter the flow cell as dam age to the flow cell may occur The flowratemay underno...

Page 22: ...n before maintenance Before inspecting and maintaining the system shut down and depressurize the sys tem and disconnect the accessories If the system is powered and pressurized accidental pump start up or unexpected pressure release can cause human injury Disconnect power Always disconnect power from the instru ment before replacing fuses Only personnel authorized by GE Healthcare may perform serv...

Page 23: ...ble rinsing liquid to remove the NaOH Disconnect power To prevent equipment damage always disconnect the power from the product before an instrument module is removed or installed or a cable is connected or disconnected ÄKTA ready Operating Instructions 28960345 AD 23 2 Safety instructions 2 1 Safety precautions ...

Page 24: ...support of the ÄKTA ready cabinet The specific data shown on this system label is only an example Actual data is specific for each individual system and may vary from system to system Note ÄKTATM ready Conforms to ANSI UL Std 61010 1 Cert to CAN CSA Std C22 2 No 61010 1 CAN ICES 1 NMB 1 24 ÄKTA ready Operating Instructions 28960345 AD 2 Safety instructions 2 2 Labels ...

Page 25: ...ction Class This symbol indicates that electrical and electronic equipment must not be disposed of as unsorted mu nicipal waste and must be collected separately Please contact an authorized representative of the manufac turer for information concerning the decommissioning of equipment The system complies with applicable European direc tives The system complies with applicablerequirements for Austr...

Page 26: ...t Description Symbol text Warning Read the user documentation before using ÄKTA ready Do not open any covers or replace parts unless specifically stated in the user documentation Warning Pinch hazard This sign is located next to pinch valves and close to the pump Never put your fingers or any objects other than the intended tubing in the pinch valve openings Ensure that clothing or other equipment...

Page 27: ...GENCY STOP button is pressed the equipment may remain pressur ized Opening a line or vessel at this point could result in the release of potentially hazardous process or cleaning fluid and cause bodily harm Whenrecoveringfrom a power failure or emergencyshutdown make sure all lines and vessels are depressurized before opening Emergency stop Pressing the EMERGENCY STOP does not automatically depres...

Page 28: ... pressing the EMERGENCY STOP on the front of the system cabinet If required also switch off the mains power supply The EMERGENCY STOP immediately stops the pump motor and sets the system in pause mode 28 ÄKTA ready Operating Instructions 28960345 AD 2 Safety instructions 2 3 Emergency procedures ...

Page 29: ...lures by en abling the system to continue to operate for a limited time A UPS is not included with the system Contact your local GE Healthcare representative for more information about the options for your specific system NOTICE If the compressed air is lost all inlet and outlet valves will close and all valves at the system front will open The pressure is let out via the air trap There is no risk...

Page 30: ...ccording to national and local environmental regulations Recycling of hazardous substances The product contains hazardous substances Detailed information is available from your GE Healthcare representative Disposal of electrical components Waste electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized represe...

Page 31: ...ment friendly Use Period EFUP which indicates the period during which the hazardous substances contained in electrical and elec tronic products will not leak or mutate under normal operating conditions so that the use of such electrical and electronic products will not result in any severe environmen tal pollution any bodily injury or damage to any assets The unit of the period is Year In order to...

Page 32: ...nent name 部件名称 PBDE 多溴二苯醚 PBB 多溴联苯 Cr6 六价铬 Cd 镉 Hg 汞 Pb 铅 0 0 0 0 0 X ÄKTA ready 29 0320 381 1 The product has not been tested as per the Chinese standard SJ T11363 2006 Requirements for Concen tration Limits for Certain Hazardous Substances in Electronic Information Product 32 ÄKTA ready Operating Instructions 28960345 AD 2 Safety instructions 2 5 Declaration of Hazardous Substances DoHS ...

Page 33: ...he technical properties of ÄKTA ready In this chapter This chapter contains the following sections See page Section 34 3 1 Overview 36 3 2 Layout 45 3 3 Flow chart 48 3 4 Equipment 52 3 5 UNICORN control system ÄKTA ready Operating Instructions 28960345 AD 33 3 System description ...

Page 34: ...to 20 liter More information can be found in Chapter 7 ÄKTA ready gradient on page 97 ÄKTA ready is not suitable for operation in a potentially explosive atmosphere or for handling flammable liquids Flow kits and columns are ordered separately See Section 14 4 Ordering information on page 210 for ordering information and lists of available flow kits and columns WARNING Do not operate the ÄKTA read...

Page 35: ...is not approved to handle flammable liquids Explosive environment The product is not approved for work in a potentially explosive atmosphere in areas classi fied as Zone 0 to Zone 2 according to IEC 60079 10 2002 The product does not fulfill the requirements of the ATEX Directive What is included in the delivery See Section 4 1 Included in the delivery on page 57 for a complete list Associated pro...

Page 36: ... 3 4 Function Part ÄKTA ready cabinet unit 1 ÄKTA ready flow kit ordered separately 2 Column trolley 3 ReadyToProcess column ordered separately 4 36 ÄKTA ready Operating Instructions 28960345 AD 3 System description 3 2 Layout ...

Page 37: ...ÄKTA ready gradient 2 1 Function Part ÄKTA ready gradient pump 1 ÄKTA ready gradient flow section 2 ÄKTA ready Operating Instructions 28960345 AD 37 3 System description 3 2 Layout ...

Page 38: ...14 15 Function ID tag Component Part Press button to pause an ongoing process Press again to continue See Pinch valves on page 48 for valve positions PAUSE CON TINUE button andalarmindi cator lamp 1 Lamp shows steady yellow light during pause Flashing yellow light indicates UNICORN alarm Shows green light when system is running No light when system is idle i e between runs or during pause RUN indi...

Page 39: ...f up to 4 bar P 201 Pump 7 A peristaltic pump is the simplest type of pump with no valves or seals that can be clogged or corroded The liquid is in contact only with the bore of the tubing eliminating the risk of the pump contaminating the liquid or the liquid contaminating the pump Peri staltic pumps can run dry 3 valve arrangement for air trap bypass in line XV 021 to XV 023 Air trap valves 8 Ho...

Page 40: ...n airtrap outlet to column inlet Allows for re moval of air in tubing between column and air trap 6 valves located on the right side of the chromatography unit XV 051 to XV 056 Outlet valves 14 The air filter is located in a holder underneath the system cabinet Air filter 15 Components on system cabinet back Location of connectors Connectors for system power pressurized air customer I O and comput...

Page 41: ...sed air connector 2 SYSTEM COMPUTER Ethernet network connector 3 CUSTOMER I O User I O 4 Instructions for connecting the system For instructions on how to connect the system see Section 4 6 Connections on page67 ÄKTA ready Operating Instructions 28960345 AD 41 3 System description 3 2 Layout ...

Page 42: ...nts of an ÄKTA ready flow kit 5 4 3 2 1 10 12 13 15 14 8 9 11 7 6 The following table gives a brief description of the flow kit components Numbers in the table refer to the figure above Several flow kit components are flow cells for sensors located on the system cabinet In such cases the ID tag technically refers to the sensors rather than the flow cells adapters Here however the tags are used for...

Page 43: ... tubing 25 mm TC connector Column inlet connector 6 Connector to column outlet tubing 25 mm TC connector Column outlet connector 7 Flow cell for pressure sensor PT 112 The sensor measures the pressure between pump and column A pH electrode can be mounted in the flow cell A dummy is placed in the electrode holder when flow kit is delivered PT 112 Flow cell for pressure sen sor 8 Sensor measuring pH...

Page 44: ... for pressure sen sor 14 Manifold with 6 outlets corresponding to valves XV 051 to XV 056 25 mm TC connec tors XV 051 to XV 056 Outlet mani fold 15 Sanitization Except for the pH electrode all parts of the flow kit are sanitized at delivery either through gamma radiation inlet manifold and main part of flow kit or autoclaving pump tubing The pH electrode should be sanitized by the user 44 ÄKTA rea...

Page 45: ...operating pressure the flow path could be pressurized to with the pressure upgrade strategy in the different parts of the system Pressure Description Part 0 6 bar Low pressure over the Inlet Manifold A 5 0 bar High pressure over the air trap manifold and column B 2 0 bar Medium pressure C 0 95 bar Medium pressure over the outlet manifold D The table below describes the system components shown in t...

Page 46: ...ed in Chapter 7 ÄKTA ready gradient on page 97 Note From inlet to column Description Stage Inlet valves open the appropriate inlet XV 001 to XV 006 in chart for sample or buffer 1 The system pump P 201 and the gradient pump P 202 deliver the liquid to the column via a pressure sensor cell PT 111 and if preferred via the air trap AT 221 where air in the liquid is removed 2 There are two sets of val...

Page 47: ...ird pressure sensor PT 113 3 Downstream from the sensors the liquid continues via the outlet tubing to the outlet manifold where valves XV 051 to XV 056 divert the liquid to either waste or fraction collection 4 Maximum pressures At the outlet manifold the system works under a pressure of max 0 95 bar Between pump and column the pressure is max 5 bar Between column and outlet manifold the pressure...

Page 48: ...er than the intended tubing in to the pinch valve opening Default position Valves Closed Inlet valves XV 001 to XV 006 Inline Air trap valves XV 021 to XV 023 Bypass Column valves XV 031 to XV 033 Closed Outlet valves XV 051 to XV 056 Closed Air Vent Valve HV 301 During pause the air trap valves will remain in the same position as during the run The user can change this by setting the air trap val...

Page 49: ... from the system pump PT 111 Measures the total pressure in the flow path after the column The difference between pres sures at PT 111 and PT 112 is the pressure drop Delta p over the column and the packed bed The second is located in the medium pressure flow path downstream from the column PT 112 Pressure sensor PT 113 protects the low pressure outlet manifold from overpressure caused by failure ...

Page 50: ...ergy the contact surfaces should be coated with a thin layer of Vaseline delivered with the flow kit or similar lubricant before the transducers are connected Air sensor The air sensors AE 151 and AE 152 are high precision monitors designed for continuous monitoring of air bubbles in the flow path When air is detected the system is either paused or performs an action that is set in the method Temp...

Page 51: ...ening nut The standard setting of the cell constant is 12 5 cm 1 UV cell The UV cell AE 131 is located after the conductivity cell in the flow path The cell consists of a transparent rectangular flow cell The cell is mounted in a holder containing a UV LED emitting at 280 nm and two detectors measuring the UV absorbance of the liquid For correct measurements it is important that UV cell and sensor...

Page 52: ...ocess control System operators are responsible for designing methods which conform to standard operating procedures and Good Manufacturing Practice procedures UNICORN is compliant with FDA 21 CFR Part 11 Knowledge prerequisite At least basic knowledge of UNICORN is expected to operate ÄKTA ready in a safe manner Information on how to use UNICORN can be obtained from the UNICORN documentation and a...

Page 53: ...includes these general steps Action Step Program an ÄKTA ready method run using the UNICORN software It is pos sible to use an existing method or modify an existing method to meet your run objectives 1 Start the run using the method you created 2 Monitor the run s progress using the System Control module All the data about your run is displayed in the System Control module You have a choice of fou...

Page 54: ... UNICORN 5 and UNICORN 7 In UNICORN 5 you start the three installation wizards Flow Kit Installation with Compo nent Test Troubleshoot Failed Component Test and Column Installation by clicking Instant Run on the File menu in the System Control module In UNICORN 7 you start the same wizards by clicking New Method on the File menu in the Method Editor module The installation wizard will create a met...

Page 55: ... prerequisites for installation of ÄKTA ready include outlets for electrical power and compressed air and access to a computer Some tools are required for unpacking the instrument and test liquids must be prepared Precautions WARNING Before attempting to perform any of the procedures described in this chapter you must read and understand all contents of the corresponding section s in the Section 2...

Page 56: ...e following sections See page Section 57 4 1 Included in the delivery 58 4 2 Site requirements 60 4 3 Transport 65 4 4 Unpacking 66 4 5 Positioning the system 67 4 6 Connections 56 ÄKTA ready Operating Instructions 28960345 AD 4 Installation ...

Page 57: ...re documentation including UNICORN and ÄKTA ready manuals system log book labels for configured voltage A 10 mm wrench for the bolts holding the pump lid is also located on the inside of the front cabinet door Associated products ÄKTA ready gradient pump is optional and used together with the ÄKTA ready gradient flow section ÄKTA ready is intended for use with ÄKTA ready flow kits ÄKTA ready Low F...

Page 58: ...sty atmosphere or close to spraying water The product is designed for indoor use only Dimensions of ÄKTA ready ÄKTA ready gradient ÄKTA ready Property 116 x 80 x 165 cm 100 80 165 cm Width Depth Height 250 kg 230 kg Weight Ambient environment Relative air humidity 20 to 95 non condensing Particle free filtered air Clean and dust free environment Temperature 2ºC to 30ºC 25ºC to 60ºC for storage Atm...

Page 59: ... 7 5 mm is supplied with the system Electrical power Delivered with a permanent power cable equipped with an IEC 60309 connector Preconfigured for 230V 50 60 Hz AC power supply See Section 4 6 Connections on page67 for instruction on how to reconfigure the power supply ÄKTA ready Operating Instructions 28960345 AD 59 4 Installation 4 2 Site requirements ...

Page 60: ...90 cm 110 c 190 cm 110 cm 115 cm 190 cm 110 cm 135 cm 190 cm Moving the crate Use a pallet jack or forklift with a minimum capacity of 400 kg to move and lift ÄKTA ready in the crate Make sure that intended openings and apertures are large enough to allow passage of the crate when lifted from the floor for transport 400 kg 60 ÄKTA ready Operating Instructions 28960345 AD 4 Installation 4 3 Transpo...

Page 61: ...an be rolled on hard and level surface with brakes on wheels released Use the handle to move ÄKTA ready Use the handle on the left side of the cabinet to maneuver ÄKTA ready ÄKTA ready Operating Instructions 28960345 AD 61 4 Installation 4 3 Transport ...

Page 62: ...00 kg for lifting the system Appropriate safety precautions should be fol lowed CAUTION Do not attempt to lift the system by the handle on the left side of the system cabinet The handle is for maneuvring the cabinet on the floor not for lifting 62 ÄKTA ready Operating Instructions 28960345 AD 4 Installation 4 3 Transport ...

Page 63: ... a dashed and dotted vertical line in the drawings below The line is located slightly towards the back of the cabinet This must be taken into consideration when moving or lifting the cabinet Note ÄKTA ready Operating Instructions 28960345 AD 63 4 Installation 4 3 Transport ...

Page 64: ...Positioning forks EU US 64 ÄKTA ready Operating Instructions 28960345 AD 4 Installation 4 3 Transport ...

Page 65: ...h steel lining Working gloves protecting against sharp edges Protective glasses WARNING Because of the significant weight of ÄKTA ready great care must be taken not to cause squeezing or crushing injuries during movement It is recommended that two persons move the system WARNING At least two persons are required to unpack ÄKTA ready gradient Instructions for unpacking For a full description of how...

Page 66: ...the cabinet must furthermore be accessible for service WARNING Access to power switch and power cord The power switch must always be easy to access The power cord must always be easy to disconnect ÄKTA ready arrives preassembled in the transport crate No specific assembly prior to site installation is required After positioning the system at its designated location lock the wheels figure NOTICE Ch...

Page 67: ...page 40 Power WARNING High voltage The mains cable must only be connected by autho rized service personnel Faulty connectionmight result in live system parts that can give lethal electric shocks NOTICE Do not turn on the Mains power switch before all connections are made Power connection The mains power supply must be connected and configured by authorized service per sonnel Please contact your GE...

Page 68: ...lts and leakage Instruction Action Step Connect your compressed air supply to the air input connector on the back of the system labeled PNEUMATIC AIR SUPPLY Use the supplied 5 m air hose with inner diameter 7 5 mm 1 Switch on the air supply 2 Check for air leaks 3 68 ÄKTA ready Operating Instructions 28960345 AD 4 Installation 4 6 Connections ...

Page 69: ...n Two network cables are delivered with the system and should be connected according to the instruction below Action Step Connect the encapsulated end of the longer 10 m straight cable to to the connector of type RJ45 marked SYSTEM COMPUTER on the back of the system 1 Connect the other end of the long cable to 2 the controlling computer if a direct connection is used or the shorter 3 m crossover c...

Page 70: ...ir sensors as described below Connection for alarm or UPS Input voltage is 24 V acceptable range 15 to28 V and output signal is 24 V max 1 A The signals are Pin 1 Remote Alarm A in figure digital output 24 V Pin 4 GND Digital ground Pin 11 UPS On B in figure digital input 24 V Pin 15 24 V Digital Supply voltage Pins 1 and 4 provide an output for an alarm device e g a warning light a buzzer or simi...

Page 71: ... connections Finish installation of ÄKTA ready by verifying that the system is properly connected to mains power to compressed air supply and to the computer Next verify that commu nication between UNICORN and the system unit can be established see instruction below ÄKTA ready Operating Instructions 28960345 AD 71 4 Installation 4 6 Connections ...

Page 72: ...ablished Switch on the computer and computer screen and check that a booting process starts 2 After booting is finished check that the UNICORN software is installed and available UNICORN symbol visible on the computer s desktop 3 Start UNICORN by double clicking its icon Check that the green POWER lamp now shows a steady green light indicat ing that UNICORN recognizes and can communicate with the ...

Page 73: ... Start Guide and scan it using the barcode reader 2 Place the barcode reader in its holder The installation is complete when it beeps 3 To verify the barcode function open an empty Notepad document type notepad in the Start menu search field click the Notepad item that is dis played as the search result Scan any barcode A text string should appear in the Notepad document 4 The Notepad document may...

Page 74: ...d in this chapter you must read and understand all contents of the corresponding section s in the Section 2 1 Safety precautions on page 15 as listed below General precautions on page 15 In this chapter This chapter contains the following sections See page Section 75 5 1 Start and log on to UNICORN 76 5 2 Start the system 74 ÄKTA ready Operating Instructions 28960345 AD 5 Starting ÄKTA ready ...

Page 75: ...n on the Windows desktop 3 When the Logon dialog box appears select a user from the Users list and enter the password 4 If you log on for the very first time select the user default and enter the password default Click OK 5 In the System Control module on the System menu click Connect 6 In the dialog shown select the appropriate system name and click OK The system name is specified during installa...

Page 76: ... After this sequence is completed the following occurs The POWER indicator lamp flashes See Components on system cabinet front on page 38 for location When communication with UNICORN software is established the POWER indicator lamp shows a steady green light When power is switched on ÄKTA ready will perform a stray light calibration without the UV flow cell installed This will result in a very hig...

Page 77: ...9 Precautions WARNING Before attempting to perform any of the procedures described in this chapter you must read and understand all contents of the corresponding section s in the Section 2 1 Safety precautions on page 15 as listed below General precautions on page 15 Flammable liquids and explosive environment on page 17 Personal protection on page 16 Operation on page 19 In this chapter This chap...

Page 78: ...mp tubing for mounting in the peristaltic pump with connectors to inlet tubing and to the main part of the flow kit tubing Pump tubing 2 Tubing with all required flow cells pressure flow meter temperature conductivity and UV air trap 6 outlets and connectors to pump tubing and column Note The pH electrode is optional For ordering informa tion see Section 14 4 Ordering information on page 210 Main ...

Page 79: ...tended for 10 to 20 liter ReadyToProcess columns ÄKTA ready Low Flow Kit with tubing diameter 6 4 mm inlet manifold 9 5 mm pump tubing 8 mm Intended for columns with a volume of less than 10 liters Both flow kits use 25 mm TC connectors for connecting to inlet and outlet containers pump tubing and column NOTICE Only use flow kits supplied by GE Healthcare Specifications for ÄKTA ready flow kits ar...

Page 80: ...e plastic bags and move them together with the other flow kit equipment to the system cabinet Open the bags immediately before mounting the flow kit onto the system cabinet 2 In addition to the plastic wrappings ÄKTA ready flow kits are equipped with plastic end caps protecting the connectors from contamination Do not remove any of the end caps until it is time to connect the flow kit to buffer ba...

Page 81: ...g of the steps in the wizard and the numbering used here You are recommended to always use the wizard when installing a new flow kit It helps to ensure that all the steps are performed and performed in the right order The wizard thereby provides a simple way of securing the correctness of a flow kit installation The wizard also includes a component test and provides the option of producing a print...

Page 82: ... is to be measured the correct flow kit size the LowFlow_Kit checkbox default or the HighFlow_Kit checkbox type of air sensor the Air_AE151 or the Air_AE152 checkbox By selecting the Service checkbox you will have the possibility to display UV_131_S and UV_131_R in RunData TC clamps Two TC clamps are provided with the flow kit An additional three clamps are required in order to mount the flow kit ...

Page 83: ...g should be prepared after mounting a new flow kit It is important that the liquids which contain acetone are freshly prepared For details about volumes and concentrations required see description in Prepare a test on page109 A Test solutionpreparationprotocol templateis availableon the ÄKTA ready CD pH calibration If pH is to be measured the pH electrode should be calibrated and sanitized before ...

Page 84: ...lation with Component Test Installation of a new flow kit normally includes a component test 2 Select the Print Report Automatically checkbox if you want to automatically print a report with installation information and test results Note A printed report can also be produced later In the Evaluation module on the File menu click Report 3 In UNICORN 7 the installationwizard creates a new method Save...

Page 85: ...the installation by turning the Installation switch in the upper right corner of the sys tem cabinet to its left position FLOW KIT INSTALL UNICORN must be in END mode 1 Open the safety locks on all pinch valves The valve safety locks are opened by turning the black handles 180 degrees There are 19 valves in total 2 ÄKTA ready Operating Instructions 28960345 AD 85 6 Flow kit installation 6 3 Mounti...

Page 86: ...ounter clockwise and the left hand bolt clockwise until both are fully open It will be neces sary to reposition the wrench 1 Check the position of the two pegs at the back of the lid The pegs indicatewhen the bolts are fully open closed 2 Lift the pump lid and remove it completely 3 86 ÄKTA ready Operating Instructions 28960345 AD 6 Flow kit installation 6 3 Mounting the flow kit ...

Page 87: ...cate the three pressure cell holders for pressure sensors PT 111 PT 112 and PT 113 1 Release but do not remove the screws on the latches and turn the latches open 2 ÄKTA ready Operating Instructions 28960345 AD 87 6 Flow kit installation 6 3 Mounting the flow kit ...

Page 88: ...e main part tubing Action Step Lift the main part of the flow kit see image using your left hand to hold the tubing by the air trap your right hand to hold the UV cell Avoid touching the window 1 Place the air trap in its holder and insert the UV cell in the slot to the right of the air trap Push the UV cell all the way in A soft click indicates that it is properly positioned 2 Place the outlet ma...

Page 89: ...it to the right 1 Push the pump tubing over the mounting knob on the right hand side of the pump 2 Stretch the tubing and push it over the left hand mounting knob 3 Using TC clamps connect the right hand connector of the pump tubing to the main part of the flow kit and the left hand connector and the sealings to the inlet manifold 4 ÄKTA ready Operating Instructions 28960345 AD 89 6 Flow kit insta...

Page 90: ... turned all the way The pegs should be in their lowermost position when the lid is closed Note that the system will not work if the lid is not properly secured 5 Note Do not turn the pump lid the wrong way around The arrow label on the lid indicates the flow direction and should point to the right The pegs should be located at the back of the lid on the side closest to the instrument 90 ÄKTA ready...

Page 91: ...ng The cable should be attached to its holder on the cabinet Pressure cells Attach the three pressure cells for sensors PT 111 PT 112 and PT 113 by pushing them onto their respective pressure cell adapters on the cabinet 2 A Secure the cells by turning the latch over the pressure cell body and fasten the screws 2 B Before firmly fastening the screws check that the cell is correctly positioned and ...

Page 92: ...ers to the connectors at the top and bottom of the flow cell see arrows in figure 3 C On the back of the flow meter cell is a window for the temperature sensor 3 in figure The window is automatically aligned with the temperature sensor when the flow meter cell is mounted No further action is necessary to pre pare for temperature measurements Note Make sure the flow meter cell is properly pushed on...

Page 93: ...onnector 2 onto the connector on the system cabinet 4 B Note Before inserting the connector make sure it is correctly positioned There is a small ridge on the male connector and a corresponding groove on the female connector Do not use force when inserting the connector ÄKTA ready Operating Instructions 28960345 AD 93 6 Flow kit installation 6 3 Mounting the flow kit ...

Page 94: ...the O ring 3 is in place in PT 112 5 D Push the pH cable through the fastening nut and connect the pH electrode AE 121 to the ca ble 5 E Insert the pH electrode in its holder Secure the electrode with the plastic fastening nut Use finger force only 5 F UV cell 6 The UV cell is mounted when the main part of the tubing is attached to the system cabinet No further actions are required to connect the ...

Page 95: ... the end of the air vent tubing 3 Note Remember to remove the protective end cap from the air vent tubing The air trap will not work properly if the cap is left in place Finishing flow kit installation Make sure that the safety locks on all pinch valves have been properly closed There are 19 safety locks in total Make sure that the three pressure flow cells are in place and properly connected Set ...

Page 96: ... a flow kit in for instance a LAF cabinet before it is mounted onto the system cabinet However if you decide to preassemble a flow kit be careful to check that the angles between the different parts are correct If they are not the flow kit will not fit properly on the cabinet possibly causing kinked tubing To adjust the different parts it is necessary to loosen the TC con nectors Connecting the fl...

Page 97: ...s chapter you must read and understand all contents of the corresponding section s in the Section 2 1 Safety precautions on page 15 as listed below General precautions on page 15 Flammable liquids and explosive environment on page 17 Personal protection on page 16 Operation on page 19 In this chapter This chapter contains the following sections See page Section 98 7 1 ÄKTA ready gradient pump 99 7...

Page 98: ...el box containing a pump motor a pump head and electrical cables It also includes a motor driver installed inside the ÄKTA ready cabinet The gradient pump is mounted on the left foot of ÄKTA ready 98 ÄKTA ready Operating Instructions 28960345 AD 7 ÄKTA ready gradient 7 1 ÄKTA ready gradient pump ...

Page 99: ...stituting the flow parts are autoclaved and ready for use with ÄKTA ready 1 2 Function Part No Doublepumptubingformountingtosystempump P 201 Pump tubing 1 Tubing to be connected to ÄKTA ready gradient pump P 202 Jumper tubing 2 ÄKTA ready Operating Instructions 28960345 AD 99 7 ÄKTA ready gradient 7 2 ÄKTA ready gradient flow section ...

Page 100: ... cations on page 204 Ordering information is in ÄKTA ready gradient flow section on page 210 Installation of gradient flow section If you have selected the ÄKTA ready gradient checkboxes in the component list see System setup on page 82 you can follow the wizard to install the ÄKTA ready gradient flow section You may also follow the instruction below More information about mounting the flow kits c...

Page 101: ...Two parts on page 99 3 Place the ÄKTA ready gradient pump tub ing from the ÄKTA ready gradient flow section package on the the ÄKTA ready system pump P 201 4 Take the jumper tubing from the ÄKTA ready gradient flow section package 5 Connect the jumper tubing to the right side of the ÄKTA ready pump tubing placed on the gradient pump using a TC clamp 6 ÄKTA ready Operating Instructions 28960345 AD ...

Page 102: ...he left side of the ÄKTA ready gradient pump tubing placed on the ÄKTA ready system pump P 201 using a TC clamp 8 Connect the B buffer tubing to the left side of the ÄKTA ready system pump tubing placed on the ÄKTA ready gradient pump P 202 inlet 7 using a TC clamp 9 102 ÄKTA ready Operating Instructions 28960345 AD 7 ÄKTA ready gradient 7 2 ÄKTA ready gradient flow section ...

Page 103: ...stable click P202Gain and shift to Gradient 0 and activate Flow_Compensation 3 Calibration procedure Pump A P 201 is ramped up to a target flow rate and the rpm value is recorded by execution of the instruction P201Gain In a second step pump B P 202 is ramped up to the same target flow rate and the actual rpm value is recorded by execution of the instruction P202Gain Then a compensation factor P20...

Page 104: ...he instructions box under FlowPath Inlet Outlet Air Trap Column 2 Use an appropriate inlet and outlet set AirTrap to Bypass and Column to Bypass 3 Connect the inlet tubing to appropriate buffer vessels 4 Start pump A B 0 with a ManFlow of 50 5 Run the pump until the flow meter signal is stable 6 Start pump B B 100 with a ManFlow of 50 7 Run the pump until the flow meter signal is stable 8 104 ÄKTA...

Page 105: ...Column under FlowPath to Inline 2 When flow signal is stable at set flow rate select P201Gain under Pump and click Execute 3 Set Gradient under Pump to 100 B and wait for a stable flow signal 4 ÄKTA ready Operating Instructions 28960345 AD 105 7 ÄKTA ready gradient 7 3 Calibrate and operate the ÄKTA ready gradient pump ...

Page 106: ...p and click ON and let the calibration procedure compensate for differences between the capacity of pumps A P 201 and B P 202 Note FlowCompensation is default set to OFF and has to be activated in each run 6 106 ÄKTA ready Operating Instructions 28960345 AD 7 ÄKTA ready gradient 7 3 Calibrate and operate the ÄKTA ready gradient pump ...

Page 107: ...re attempting to perform any of the procedures described in this chapter you must read and understand all contents of the corresponding section s in the Section 2 1 Safety precautions on page 15 as listed below General precautions on page 15 Flammable liquids and explosive environment on page 17 Personal protection on page 16 Operation on page 19 In this chapter This chapter contains the following...

Page 108: ...hange anything on the system or in UNICORN during the time ap proximately 10 seconds stray light is being measured Also note that if stray light calibration is included in a user defined method a delay of at least 0 3 minutes is required before the next instruction 2 After the air trap has been filled with the chosen liquid the function of the flow meter cell is tested if the test fails the test p...

Page 109: ...e on the system CD The volumes depend on the flow kit used ÄKTA ready High Flow Kit 25 liters of wash liquid and 10 liters of test solution ÄKTA ready Low Flow Kit 10 liters of wash liquid and 5 liters of test solution Test solution for the High Flow Kit is prepared as follows 10 liters of 1 0 M NaCl with 1 acetone in purified water i e 584 g NaCl 100 ml acetone in 9 90 liters purified water For t...

Page 110: ...inlet tube 7 2 Remove the protective end cap from outlet 6 arrow and connect a waste hose to this outlet 3 WARNING Over pressure Never block the outlet tubing with for instance stop plugs since this will create over pressure and might result in injury 110 ÄKTA ready Operating Instructions 28960345 AD 8 Component test 8 1 Run a component test ...

Page 111: ...here is also a field for the test solution protocol number Much of the information can be conveniently scanned in using the supplied bar code scanner It can also be manually entered The information supplied will be included in the report Result name The panel Result Name contains fields for the name of and path to the result file where results will be stored Enter a proper name for the result file...

Page 112: ...cking Acknowledge or not The warning message can be disabled see Manual calibration of UV stray light on page 148 A few minutes into the test a message will appear prompting the user to fill the air trap This message requires user action Press the AIR VENT button to open the air vent valve HV 301 allowing for evacuation of air from the air trap You must respond to the message regarding evacuation ...

Page 113: ...er containing basic information is shown at the top of each report page The in formation includes the UNICORN version number the name of the person who ran the test and the time and date for the test Also the path for the result file is shown in the header Questions The Questions section contains identification information This is the same information as that entered in the Questions panel in UNIC...

Page 114: ...uation module click View click Run Record and then click the Notes tab In the UNICORN 5 Evaluation module or the UNICORN 7 Evaluation Classic module on the View menu click Documentation and then click the Notes tab 114 ÄKTA ready Operating Instructions 28960345 AD 8 Component test 8 2 The test report ...

Page 115: ...are replaced by text describing the result of the test In both UNICORN 5 and UNICORN 7 chromatograms can be found in the result file Note A vertical text on each chart shows the acceptance interval used and also whether the component in question passed the test or not If the test for a component fails troubleshooting should be done see Section 8 3 Trou bleshooting a failed component test on page 1...

Page 116: ...which there is room for comments on the paper copy of the report The page also contains the signature fields Performed by and Approved by where date name and position can be specified 116 ÄKTA ready Operating Instructions 28960345 AD 8 Component test 8 2 The test report ...

Page 117: ...iously described Open the installation wizard Action Step 1 UNICORN 5 In the ÄKTA ready System Control module on the File menu click Instant Run and then click Run in the dialog box shown UNICORN 7 In the ÄKTA ready Method Editor module on the File menu click New Method Click Method Wizard in the New Method dialog box and then click OK In the wizard click Troubleshoot Failed Component Test Select ...

Page 118: ...ces of the transducer connectors see Mount the main part tubing on page 91 Transducersurfacesnot treated with Vaseline Check that the pump tubing has been correctly mounted and that the pump lid is properly secured Make sure the pegs on the pump lid are turned all the way down see Connect and mount the pump tubing on page 89 Incorrectly mounted pump tubing Make sure the TC clamps connecting the pu...

Page 119: ...meter cell see Mount the main part tubing on page 91 Possible problems with temperature measurement are Errors in temperature readings Loose flow meter cell Check that the flow meter cell is properly snapped onto its mounting knobs see Mount the main part tubing on page 91 A gap be tween the temperature window and the sensor may cause incorrect measurements Dirty or damp temperature window Wipe cl...

Page 120: ...quid is connected to Inlet 6 and the test liquid to Inlet 5 Wrong inlets used Make sure that the liquids used are fresh and correctly prepared see Prepare a test on page 109 Wrong composition of wash liquid and or test solution Make sure the temperature of the process liquid not is lower than 10ºC from the ambient temperature Condensation of mois ture on the flow cell window 120 ÄKTA ready Operati...

Page 121: ...uid from the air trap also inlet and outlets that are cur rently not used To avoid spillage of liquid do not remove the end caps from connections that are not used If part of the tubing has not been properly inserted in a valve Tubing not properly in serted 1 Turn the Installation switch to position FLOW KIT INSTALL 2 Open the valve lock 3 Insert the tubing 4 Close the valve lock 5 Turn the instal...

Page 122: ...be performed in connection with installing a new column Note that ReadyToProcess columns are factory tested and are supplied with a certificate stating the results from an efficiency test performed on the individual column Precautions WARNING Before attempting to perform any of the procedures described in this chapter you must read and understand all contents of the corresponding section s in the ...

Page 123: ...wing sections See page Section 124 9 1 Columns 127 9 2 Installing a column 135 9 3 Optional column test 138 9 4 Optional column rinsing 139 9 5 Adding columns to the column list ÄKTA ready Operating Instructions 28960345 AD 123 9 Column installation ...

Page 124: ...r types of columns may be used but may require special safety precautions More information about columns and separation media can be found in the Product catalog on the GE Healthcare web page address on back cover 124 ÄKTA ready Operating Instructions 28960345 AD 9 Column installation 9 1 Columns ...

Page 125: ...anitized MabSelect SuRe MabSelect SuRe LX Butyl S Sepharose 6 Fast Flow Blue Sepharose 6FF CM Sepharose FF DEAE Sepharose FF Phenyl Sepharose 6 Fast Flow low and high sub Q Sepharose FF SP Sepharose FF ReadyToProcess columns are available in the following sizes 1 l i d 80 mm bed height 200 mm 2 5 l i d 126 mm bed height 200 mm 10 l i d 251 mm bed height 200 mm 20 l i d 359 mm bed height 200 mm ÄKT...

Page 126: ...ith ÄKTA ready other columns may be used provided they are capable of withstanding maximum system pressure If not separate pressure safety devices must be used WARNING High pressure The flow rate may under no circumstances exceed the specified column maximum flow rate High flows might affect the packed medium causing the pressure to ex ceed the specified column maximum pressure Over pressure Never...

Page 127: ...struc tions for the chosen column Preparation Before installing a column the flow kit must be filled with liquid e g distilled water from inlet 6 to the column valves including the air trap If the column is installed after a component test the flow kit is automaticallyfilled and no further preparation is necessary ÄKTA ready Operating Instructions 28960345 AD 127 9 Column installation 9 2 Installi...

Page 128: ...test on page135 and rinsing in Section 9 4 Optional column rinsing on page 138 3 Select the Print Report Automatically checkbox if you want to automatically produce a printed report with installation information and test results Note A printed report can also be produced later In the Evaluation module on the File menu click Report 4 Similar to the wizard for installing a new flow kit the column in...

Page 129: ...type specific values found in the user documentation for the column See Edit column parameters in UNICORN 7 on page 130 Note Action Step From the Technique list in the Initial Settings pane select a chromatographic technique from the alternatives All Affinity Anion exchange Cation ex change and Hydrophobic Interaction The selection limits the list of columns available for selection in next step De...

Page 130: ...Roll the trolley into position between the system cabinet leg supports and lock the trolley wheels Note Be careful when transporting smaller columns e g 2 5 l on the trolley Small columns are light and have a narrow base and may therefore be unstable 3 Click Next in the column installation wizard This will open a checklist of ac tions for connecting the column 4 Edit column parameters in UNICORN 7...

Page 131: ... fit the column and media used See PID control in ÄKTA ready on page 215 and the user documentation for the column Tip Enter values for Flow_Efficiency_Test l hour and Rinsing_Flow_Rate l hour corresponding to a linear flow of 100 cm h for the column used 3 Tip Column parameter values can also be found in ReadyToProcess columns User Manual ÄKTA ready Operating Instructions 28960345 AD 131 9 Column...

Page 132: ...e tubing ends for when the column shall be sealed 1 If necessary fill the inlet tubing with 20 ethanol or 2 benzyl alcohol using e g a squirt bottle 2 1 2 Remove the protective end cap from the column inlet tubing on the flow kit 1 in figures and using a TC clamp connect it to the Bottom Inlet tubing on the column 3 Repeat the steps above for the Column outlet connector 2 in figures Connect this t...

Page 133: ...rn the Installation switch in the upper right corner of the system cabinet to the RUN posi tion 3 After the column installation wizard has finished UNICORN displays the start protocol with information about the installation and input fields for parameters specifying the current setup The input fields are the same as those for a flow kit installation as discussed in Enter installation information o...

Page 134: ...lly produced or manually created by se lecting the report format AKTA ready_Column in the Report dialog box in the UNICORN Evaluation module The report format AKTA ready_Column_T also contains results from an efficiency test see next section 134 ÄKTA ready Operating Instructions 28960345 AD 9 Column installation 9 2 Installing a column ...

Page 135: ...n 10 3 Creating a column efficiency test on page 168 Note Preparations When selecting the Column Installation alternative in the column installation wizard the user is given the choice of also selecting the Include Efficiency Test of Column checkbox see Section 9 2 Installing a column on page127 By doing so additional items concerning preparation and connection of wash and test liquids will be inc...

Page 136: ...mately 30 minutes If column conditioning is included in the test it will take longer time than 30 minutes Tip Similar to a component test it is possible to enter notes during the test see Running the component test on page 112 For more information about efficiency testing of columns see the application note Effi ciency test of ReadyToProcess columns see Section 1 3 Associated documentation on page...

Page 137: ...est report also contains detailed infor mation about the column used e g volume technique max and default flow rates etc A column test report contains a chart showing the peak in UV response caused by the progressing pulse of tracer solution together with suggested acceptance criteria Unlike a component test report a column test report does not state whether a column has passed or failed a test In...

Page 138: ... include a field for selecting the inlet used for the rinsing liquid The rinsing liquid may consist of for instance purified water or a low salt buffer see column documentation for details The volume required depends on the column used Note that the liquid used for rinsing may be the same as that used as wash liquid in a previous efficiency test see Section 9 3 Optional column test on page135 In s...

Page 139: ...lect a column supported by the ÄKTA ready software i e a ReadyTo Process column with the prefix RTP in the list that has the same column volume and average particle diame ter as the column to be added marked items in figure This will set the advanced parameters that are compatible with the software 2 Click Edit for the selected column 3 Enter appropriate parameter values for the column to be added...

Page 140: ...Action Step The added column is now be available in the ÄKTA ready wizard 6 140 ÄKTA ready Operating Instructions 28960345 AD 9 Column installation 9 5 Adding columns to the column list ...

Page 141: ...ethods for efficiency testing of columns Note that the discussion in this chapter applies to methods written for the ÄKTA ready strategy For information about methods in general and for detailed information about the Method Editor please see the UNICORN manuals Courses in UNICORN system control including method design are available from Fast Trak Training Education Contact your local GE Healthcare...

Page 142: ...ains the following subsections See page Section 143 10 1 1 Introduction to the Method Editor 145 10 1 2 Method Editor layout 147 10 1 3 Other Method Editor features 142 ÄKTA ready Operating Instructions 28960345 AD 10 Creating a method 10 1 The Method Editor ...

Page 143: ...g methods and also allow for reuse of existing sets of instructions Individual blocks can be moved and used in any method within the same strategy as required Creating a block The general procedure to create a block in the Method Editor is to 1 Click New on the Block menu 2 In the dialog box shown give the block a name and select a base Time Volume or Column volumes for the block The choice of bas...

Page 144: ...ethod Editor window now shows the Text pane and the lower part the Instructions box It is possible to also show an op tional flow diagram or a block dia gram in the Method Editor by select ing the appropriate items in the Cus tomize panes dialog box These dia grams can help when programming advanced methods but are not re quired for building or editing a method Theyarethereforenotinclud ed in the ...

Page 145: ...s box The parameters for the block or instruction that is currently selected in the Text pane are displayed and available for editing in the Instructions box in the lower part of the Method Editor window The Instructions box is divided into four areas These are from left to right Breakpoint Contains a field for setting the breakpoint for the instruction or block in the unit currently selected as b...

Page 146: ...ion Note Instructions placed at the same breakpoint will execute simultaneously while blocks will execute in the order they occur in the Text pane Changing valve positions or changing the speed of the pump does not take place mo mentarily but requires a small amount of time A small time lag such as 0 10 min should therefore be introduced after time based instructions affecting mechanical component...

Page 147: ...eate variables representing breakpoints or parameters Variables enables re use of defined breakpoints and instructions in other blocks UV stray light calibration There is an instruction to perform a stray light calibration When a new flow kit is mounted the instruction is activated automatically via the installation wizard It may also be included in the methods if so needed The instruction Strayli...

Page 148: ...f you click Execute the stray light calibra tion will be performed and a warning message is displayed Stray light reduction calibration Do not perform any other instruction during the next 15 seconds Click Acknowledge when 15 seconds have passed If you click Disabled and after that Execute the stray light calibration will be performed without the warning message on the screen and without entry in ...

Page 149: ...tem menu Click Alarms Mon and scroll down to Fine_Tune_Fail_Report 1 If you click Enable and after that Execute the Fine_tuning_flowme ter_failed warning will be displayed if the system fails to perform a fine tuning If you click Disable and after that Execute the Fine_tuning_flowme ter_failed warning will not be displayed on the screen and there will be no entry in the logbook ÄKTA ready Operatin...

Page 150: ...ng of needed actions triggered by specific events To define an action based on the totalizer for for instance Inlet 1 1 Enable the totalizer by inserting instruction Inlet1_Totalizer under FlowPath and setting it to Enabled 2 Insert watch instruction Watch_Inlet1_Totalizer under Watch and set values for Test e g Greater_Than Value volume in liters and Action e g END_BLOCK To activate an alarm for ...

Page 151: ...ressureFlowLimit 1 Select the pressure and the pressure sensor that should control the pressure 2 Select the flow rate and the pressure flow control will work as expected 3 Deactivate the function and select a flow to change system status to flow mode 4 ÄKTA ready Operating Instructions 28960345 AD 151 10 Creating a method 10 1 The Method Editor 10 1 3 Other Method Editor features ...

Page 152: ...n This section contains the following subsections See page Section 153 10 2 1 Creating the method and selecting a column 154 10 2 2 Block 1 Opening valves 156 10 2 3 Block 2 Auto zeroing pressure sensors 157 10 2 4 Block 3 Setting PID parameters 158 10 2 5 Block 4 Filling the flow kit 160 10 2 6 Block 5 Equilibration 161 10 2 7 Block 6 Sample load 163 10 2 8 Block 7 Washing unbound sample 164 10 2...

Page 153: ...that ReadyToProcess columns start with RTP in the list 3 Set the Flow_Warning instruction to Disable and click Insert This action will avoid repeated display of the warning message No flow in begin ning of method The flow warning will pop up since inlet and outlet must be set before flow can be started It is also crucial to use Time as base in this type of block because if Volume is chosen and no ...

Page 154: ... new block will show up in the Text pane with the specified name and base Insert an instruction that opens the valve for inlet 6 In the Text pane select the Base instruction and in the Instruction Box set the Inlet instruction under FlowPath to Inlet 6 The Breakpoint time should be 0 00 Insert the instruction into the block by clciking Insert 3 The new instruction will show up in the Text pane bel...

Page 155: ...set instruction Outlet to Outlet6 The block is now finished All the in structions are available for reviewing in the Text pane see figure If you want to modify an instruction select it and make the changes in the Instructions box ÄKTA ready Operating Instructions 28960345 AD 155 10 Creating a method 10 2 An example of using the Method Editor 10 2 2 Block 1 Opening valves ...

Page 156: ..._PT112 and AutoZero_PT113 in group Alarms Mon at time 0 10 2 Note This requires that the system is filled with air If it is filled with liquid the air trap valve has to be opened by pressing the AIR TRAP VENT button with outlet closed and air trap inline The MyAutozeroPressure block now contains three Autozero instructions one for each pressure sensor 156 ÄKTA ready Operating Instructions 28960345...

Page 157: ...ction Step Create a new block for setting of PID parameters Name the block MyPIDPa rameters and set Time as its base 1 Insert the FlowTune instruction into the block This instruction has the re quired fields for setting PID parameters 2 There are six parameters in total three each for the High Flow Kit and the Low Flow Kit When running the method only those parameters relevant for the flow kit ins...

Page 158: ... noise level for flow 2 Insert the instruction Man_Flow in group Pump at time 0 00 The flow is set to 30 The Man_Flow instruction will start pumping liquid through the flow path at a steady rate 3 Define a Hold_Until instruction in group Watch at time 0 10 Set the Signal parameter to Flow_FT141 refers to the flow meter set the Test criterion to Stable_Baseline and set Minutes to 0 5 which means th...

Page 159: ...on with the flow set to 50 at volume 0 0 4 Insert a Message instruction containing a message to the user that the AIR VENT button must be pressed in order to fill the air trap Set the message to appear on Screen and add a Sound signal if so desired 5 Set the End_Block instruction for the nested block at volume 2 50 which means that the block will finish when the pump has delivered 2 5 liters of li...

Page 160: ...e alarm is Enabled 2 Add a Column instruction and set the column Inline 3 Set a Flow instruction to 300 cm h by first selecting Linear flow to change unit and then typing 300 In sert the instruction at 0 0 column volumes 4 Set End_Block to 3 00 which means that the equilibration will finish after 3 column volumes To achieve reasonable equilibration at least 1 5 column volumes should be pumped thro...

Page 161: ... set the time and volume to zero when the sample inlet valve is opened 4 In order to fill the flow path with sample liquid start a manual flow which is stopped when the sample reaches the UV sensor i e when the UV readings reach above a defined limit The two instructions required for this are 5 ManFlow in group Pump which starts the flow and maintains it at a set level in this case 50 ManFlow is n...

Page 162: ...ers of sample after first having au to zeroed UV set an injection mark and filled the flow kit with sample liquid 162 ÄKTA ready Operating Instructions 28960345 AD 10 Creating a method 10 2 An example of using the Method Editor 10 2 7 Block 6 Sample load ...

Page 163: ...et to for instance Inlet6 2 Insert a Hold_Until instruction that is triggered when UV readings are Less_Than a set limit e g 100 mAU At this point the flow path is consid ered to be clean and the block will finish 3 The MyWashOutSample block first switches to the inlet of the washing liquid and then washes the flow path until the UV readings indicate it is clean ÄKTA ready Operating Instructions 2...

Page 164: ... 8 liters and set the fraction outlet to Outlet2 3 Once defining the Fraction block is complete right click the block and click Delete A di alog box is displayed in which you can choose to Delete or Move the block click Move The block will be stored as Unused in the location you select and will be available for insertion in any method The Fraction_Stop block The procedure for creating the Fraction...

Page 165: ...etting the Test parameter to Greater_Than and the Value parameter to 100 mAU In the popup menu under Action select the Fraction block that you previously created 3 Using the same procedure as in the previous step create another watch instruction for stopping fractionation when UV goes below 90 mAU Set the parameter Test to Less_Than and Value to 90 mAU and select the previously created Fraction_St...

Page 166: ...lumn volumes and is defined in a nested block The procedure is very similar to that of defining a block for washing unbound sample see Section 10 2 8 Block 7 Washing unbound sample on page163 When as in this case a similar block already exists time can be saved by copying the existing block renaming it and changing the parameter values as required 166 ÄKTA ready Operating Instructions 28960345 AD ...

Page 167: ...Method Editor to open the Save as dialog box In this dialog box you specify the folder in which to save the method and also the system and technique it is intended for ÄKTA ready Operating Instructions 28960345 AD 167 10 Creating a method 10 2 An example of using the Method Editor 10 2 11 Saving the method ...

Page 168: ...ORN Method Editor module by clicking Method Wizard on the File menu Running the wizard In the method wizard dialog box select col umn and the inlets to be used for wash liquid and tracer solution The method variables will be set according to the choice of column Click Finish to leave the method wizard 168 ÄKTA ready Operating Instructions 28960345 AD 10 Creating a method 10 3 Creating a column eff...

Page 169: ...default values of variables are Flow velocity 100 cm h Equilibration volume 1 30 column volumes Tracer volume loaded on the column 2 5 of the column volume For the method to work correctly changing of the flow velocity requires changing of the tracer volume and vice versa Note Only advanced users should change variables shown in the detailed section only displayed when Show details is checked Note...

Page 170: ...ttempting to perform any of the procedures described in this chapter you must read and understand all contents of the corresponding section s in the Section 2 1 Safety precautions on page 15 as listed below General precautions on page 15 Personal protection on page 16 Operation on page 19 In this chapter This chapter contains the following sections See page Section 171 11 1 Final preparations befo...

Page 171: ...ed Follow local and or national regulations for safe operation and maintenance of ÄKTA ready Corrosive substance NaOH is corrosive and therefore danger ous to health When using hazardous chemicals avoid spillage and wear protective glasses and other suitable Personal Pro tective Equipment PPE CAUTION Do not use chemicals at temperatures above the specified limits ÄKTA ready Operating Instructions ...

Page 172: ...t during the run Set valve switch time for inlet and outlet valves The UNICORN control strategy features a short overlap in valve switching at the inlet and outlet valves This overlap allows for continuous operation of the pump without the risk for blockage of the flow path due to a closed inlet or outlet manifold When operating the system and switching from one inlet to another from one outlet to...

Page 173: ...at the column has been equilibrated if this is not included in the method Check flow kit Check that the flow kit is of the same type as that entered in the System setup dialog box in UNICORN Manager in UNICORN 5 or in the Edit System dialog box in UNICORN 7 see Before starting on page 82 Calibrate flow meter It is recommended that the flow meter is always calibrated when the flow kit has been chan...

Page 174: ...ap can only remove small amounts of air If air reaches the flow meter during a run the alarm Uncertain flow will be displayed and the system will enter pause mode Priming should be done for each inlet starting with inlet 1 Use the ManFlow command to control the pump during priming 174 ÄKTA ready Operating Instructions 28960345 AD 11 Run a method 11 1 Final preparations before a run ...

Page 175: ...ocol is displayed consisting of a number of dialog boxes On the Variables page it is possible to fine tune the method before proceeding Selecting the Show details checkbox will display more detailed information Check that the sample volume is correct 3 Click Next or Back to navigate through the dialog pages adding the infor mation required as well as your own comments 4 Click START in the Result N...

Page 176: ...ayout of a panel right click and then click Properties By clicking Documentation and then Run notes it is possible to add notes during a run Entered notes will be included in the results More information regarding the monitoring possibilities that the UNICORN user interface offers is available in the UNICORN User Manual 176 ÄKTA ready Operating Instructions 28960345 AD 11 Run a method 11 2 Startin...

Page 177: ...ep running In the dialog box you can choose to save the partial results from the run so far If the run is part of a scouting run you are also given the choice of ending this If you do not the next run will start automatically Emergency stop In case of emergency stop the run by pressing the EMERGENCY STOP button This will immediately stop the pump and close all inlet and outlet valves To restart th...

Page 178: ...ble click the result file The result file opens in a Chromatogram panel 2 To change the layout of the Chromatogram panel right click and and then click Properties Select which curves to display by marking them in the Curve panel Click OK 3 Refer to the UNICORN manuals for more information about viewing results and customiz ing layout 178 ÄKTA ready Operating Instructions 28960345 AD 11 Run a metho...

Page 179: ...ect print format and layout op tions 3 Click Preview 4 In the Customise Report window verify that the layout is correct 5 Click Exit to return to the Print Chromatograms dialog box 6 Click OK 7 Refer to the UNICORN User Manual for more information about printing results ÄKTA ready Operating Instructions 28960345 AD 179 11 Run a method 11 3 Viewing and printing results ...

Page 180: ...umn with water This is done by connecting water to one or more of the inlets and running a washing method from the ÄKTA ready 1 Fill the flow kit and column with air by pumping air from an empty inlet 2 Note Use ManFlow when filling the system with air The flow meter which is used to control the pump does not work with air in the system Disconnect all inlet and outlet containers and the column and...

Page 181: ...oclave the flow kit contained in for example an autoclave bag Remove the protective caps before autoclaving 10 Dispose of the empty flow kit and column in accordance with local regula tions Also consult the instructions in the column documentation 11 ÄKTA ready Operating Instructions 28960345 AD 181 11 Run a method 11 4 After the run ...

Page 182: ...ures described in this chapter you must read and understand all contents of the corresponding section s in the Section 2 1 Safety precautions on page 15 as listed below General precautions on page 15 Flammable liquids and explosive environment on page 17 Personal protection on page 16 Operation on page 19 Maintenance on page 22 WARNING Only personnel authorized by GE Healthcare may perform service...

Page 183: ... before replacing fuses NOTICE Disconnect power To prevent equipment damage always discon nect the power from the product before an instrument module is removed or installed or a cable is connected or disconnected In this chapter This chapter contains the following sections See page Section 184 12 1 Daily maintenance 187 12 2 Yearly maintenance 194 12 3 Spare parts 195 12 4 Storage ÄKTA ready Oper...

Page 184: ...ludes cleaning and calibration of the pH electrode WARNING Electrical shock hazard All installation service and maintenance of components inside the electronics cabinet should be done by service personnel authorized by GE Healthcare Do not open any covers or replace parts unless specifically stated in the Operating Instructions 184 ÄKTA ready Operating Instructions 28960345 AD 12 Maintenance 12 1 ...

Page 185: ...fore service or maintenance Make sure that the cleaning procedure flushes all possible flow paths in the system If liquid should have accidentally spilled into the pump clean the inside of the pump lid and the rollers with water To avoid corrosion make sure all surfaces are wiped dry The interior of the pump is particularly sensitive to NaOH which corrodes the aluminum After cleaning make sure the...

Page 186: ...he System menu click Cali brate 3 Place the pH electrode in one of the buffers enter the pH value for the buffer in the Reference value 1 field wait for the value to stabilize and click Read value 1 4 Rinse the electrode in distilled water and place it in the other buffer solution Enter the pH value for the buffer in the Reference value 2 field wait for the value to stabilize and click Read value ...

Page 187: ...l contact your local GE Healthcare representa tive Sensors Sensors are checked and if necessary changed by service personnel as part of the yearly service Should a warning be displayed in UNICORN concerning one of the sensors please contact GE Healthcare contact information on back cover Calibrating flow meter Flow readings that differ from zero under no flow conditions e g when system is in its E...

Page 188: ...ressure calibration cell article 28 9329 42 in Section 14 4 Ordering information on page210 and a suitable pump The recommended pump is DPI 603 from GE Sensing Calibration is best performed with no flow kit mounted Calibration includes the following steps Action Step Mount the calibration cell on pressure meter PT 111 figure shows calibra tion cell with pump tubing removed 1 Cycle the pressure cel...

Page 189: ...tion of the conductivity sensor is normally not necessary in UNICORN because a standard setting of the cell constant is used Note The procedure for calibrating the conductivity sensor is shown in the following steps Action Step Prepare a calibration solution of 1 00 M NaCl 58 44 g 1000 ml Let the solu tion reach room temperature This is important for exact measurements 1 Settemperaturecompensation...

Page 190: ...displayed value and the theoretical value correspond no further action is required If the values differ proceed with step 7 6 Enter the theoretical conductivity value according to the graph in the Reference value 1 field 7 Click Read value 1 The new cell constant is automatically calculated and updated 8 Testing the temperature sensor The accuracy of the temperature sensor can be checked using a T...

Page 191: ...ed in UNICORN Temp_161 Calculate the deviation 4 If the temperature deviation is greater than that given in the specification Section 14 4 Ordering information on page210 contact you local GE repre sentative 5 Testing the UV LED Action Step Prepare three acetone solutions with deionized water 1 0 5 weight percent 1 2 Connect a flow kit or use the UV cell test tool part of ÄKTA ready UV Cond test t...

Page 192: ...efore check the condition of the air filter regularly If the filter is dirty change it as follows Action Step Locate the filter beneath the cabinet front panel arrow in figure 1 Remove the filter cover and filter by unscrewing the six knurled nuts see lower figure No tools should be nec essary 2 Wipe clean the bottom surface of the cabinet and the filter cover 3 Place the new filter in the cover a...

Page 193: ...pump or of vibrations as these may indicate that the pump rotor needs to be replaced Normally the pump rotor is checked and replaced in connection with yearly maintenance ÄKTA ready Operating Instructions 28960345 AD 193 12 Maintenance 12 2 Yearly maintenance ...

Page 194: ... with other components when opened Use only finger force to tighten the screws Air filter nuts and cover The knurled nuts holding the air filter cover are mounted as described on Air filter on page 192 Wheels The wheels are screwed onto the system cabinet support from below This requires that the cabinet is lifted slightly Before attempting to replace a wheel carefully read the in structions in th...

Page 195: ... 25 C and 60 C room temperature is preferable Note Flow kits should not be stored at temperatures below 5 C Do not leave a flow kit mounted on the system for an excessively long time The tubing walls may stick together where the tubing is squeezed possibly causing high pressure alarms when running the system NOTICE Do not use 0 5 M NaOH or higher for more than 2 hours Flush afterwards with a suita...

Page 196: ... by a failing power supply or pressurized air If the suggested actions in this guide do not solve the problem or if the problem is not covered by this guide contact your GE Healthcare representative Precautions WARNING Before attempting to perform any of the procedures described in this chapter you must read and understand all contents of the corresponding section s in the Section 2 1 Safety preca...

Page 197: ...e during a run Contactbetweencomput er and system is broken Check circuit breaker both in system and for external supply as applicable Note If the power failure is local affecting only the computer the system will continue to run while locally storing the data Stored data are transferred to the computer as soon as communication with the computer is restored Power failure during a run Power Note If...

Page 198: ...e Note that small amounts of air are handled by the air trap Larger amounts reaching the flow meter cell cause Uncertain flow alarm The warning message means that the inaccuracy of the flow meter is maximum 1 unit higher than speci fied Warning Fine tuning flow meter failed is displayed Flow meter The lubricant between the flow meter cell and the transducers may be insufficient Lubricate according...

Page 199: ... kit Kinks or blockages in the flow kit Remount or re place flow kit See Section 6 3 Mounting the flow kit on page 81 for mounting instructions Pump rotor slipping on the drive shaft contact GE Healthcare service personnel Little or no flow Liquid too viscous and or pump speed too slow Check that viscosity of liquid is within specified range see Section 14 1 Specifications on page202 Increase pump...

Page 200: ... 2 Press CONTINUE in UNICORN or on the system cabinet 3 Press the AIR VENT button on the system cabinet Condensation of moisture on the flow cell window The process liquid is too cold must not be lower than 10ºC from ambient temperature Drifting UV signal UV UV signal does not return to zero Stray light calibration performed without cell UV absorption values 5 AU even without flow kit installed In...

Page 201: ...dering infor mation In this chapter This chapter contains the following sections See page Section 202 14 1 Specifications 206 14 2 Process wetted materials 207 14 3 Chemical resistance 210 14 4 Ordering information 214 14 5 PID control 216 14 6 Checklists 221 14 7 Health and Safety Declaration Form 223 14 8 More information ÄKTA ready Operating Instructions 28960345 AD 201 14 Reference information...

Page 202: ...240 V 10 1x100 120 200 208 220 230 240 V 10 AC voltage 50 60Hz 50 60Hz Frequency 1 kVA 1 kVA Max power consumption IP45 pump IP25 IP45 pump IP25 Ingress protection 5 5 to 10 bar 50 Nl min oil and particle free 5 5 to 10 bar 50 Nl min oil and particle free Compressed air interface Recommended operating conditions Value Property 2ºC to 30ºC Ambient temperature 2ºC to 40ºC Liquid temperature 950 to 1...

Page 203: ... 8 Inlet manifold diame ter i d 12 mm 8 mm Pumptubingdiameter i d 7 5 to 510 l h 3 to 175 l h Flow rate range 2 145 l 0 762 l Total volume 1 619 l 0 502 l Air trap volume TC 25 TC 25 Connectors type size 6 6 6 6 No of inlets outlets 340 rpm 225 rpm Max pump speed Pressure rating 0 6 bar low pressure inlet manifold 5 0 bar high pressure flow path upstream of col umn 2 0 bar high pressure flow path ...

Page 204: ... h 3 to 175 l h Flow rate range 0 116 l 0 055 l Total volume TC 25 TC 25 Connector i d 2 C to 30 C 2 C to 30 C Ambient temperature 2 C to 40 C 2 C to 40 C Liquid temperature 3 years 3 years Estimated shelf life 5 bar g 72 5 psi 0 5 MPa 5 bar g 72 5 psi 0 5 MPa Maximum operating pres sure 204 ÄKTA ready Operating Instructions 28960345 AD 14 Reference information 14 1 Specifications ...

Page 205: ...n earity 5 7 8 0 01 to 1 0 AU 7 to 7 AU UV 3ºC 2ºC to 40ºC 0 to 47 C Temperature 1 Max error valid within acceptance range under operation conditions 2 Flow meter cells for High Flow Kit and Low Flow Kit are designed with different flow path ge ometries 3 Valid for max temperature difference of 10 ºC between liquid and ambient temperature 4 Temperature compensation optional The standard setting of...

Page 206: ...aterials Materials Component Silicone platinum cured reinforced tubing in high pressure flow path Tubing Composite of expanded Polytetrafluoroethylene PTFE and platinum cured silicone Pump tubing Polypropylene PP polyether ether ketone PEEK Connectors Polymethylpentene TPX Flow meter body Polyamide PA transparent Polypropylene PP Air trap Polyoxymethylene POM ceramics junction glass pH electrode P...

Page 207: ...lutions for adsorption elution and washing and to solutions effective in cleaning and storage The following table lists chemicals that may be used with ÄKTA ready The concentrations listed are not normally exceeded during an operating cycle The information in the table below is valid for a maximum exposure time of 48 hours The table should be used only as a guide The effects of a chemical will gen...

Page 208: ...l PEG 500 1 Polyethylene glycol PEG 1000 1 Polyethylene glycol PEG 5000 1 M Potassium phosphate 5 1 propanol 30 2 propanol 1 SDS 1 M Sodium acetate 4 M Sodium chloride 1 M Sodium citrate 1 M Sodium sulfate 0 5 M Maximum exposure time 2 hours Sodium hydroxide 1 M Sorbitol 1 M Sucrose 1 Triton X 100 1 Tween 20 1 Tween 80 208 ÄKTA ready Operating Instructions 28960345 AD 14 Reference information 14 3...

Page 209: ...Concentration Chemical allowed 8 M Urea ÄKTA ready Operating Instructions 28960345 AD 209 14 Reference information 14 3 Chemical resistance ...

Page 210: ...w Flow Kit ÄKTA ready gradient flow section Product code Inner diameter mm Product 29021086 9 5 ÄKTA ready gradient High Flow section 29021085 6 4 ÄKTA ready gradient Low Flow section ÄKTA ready accessories spare parts etc Product code Product 18116877 pH electrode 28931880 Column trolley 18116958 Air filter 28930915 Pump track assembly pump lid 28929887 Pressure cell holder kit latch and screws 2...

Page 211: ...10 RTP Capto adhere 10 28901716 20 RTP Capto adhere 20 28951118 1 RTP Capto MMC 1 Capto MMC 28929120 2 5 RTP Capto MMC 2 5 28929121 10 RTP Capto MMC 10 28929122 20 RTP Capto MMC 20 28951090 1 RTP Capto Q 1 Capto Q 28901723 2 5 RTP Capto Q 2 5 28901724 10 RTP Capto Q 10 28901725 20 RTP Capto Q 20 28951093 1 RTP Capto S 1 Capto S 28901729 2 5 RTP Capto S 2 5 28901730 10 RTP Capto S 10 28901731 20 RT...

Page 212: ...01737 20 RTP Phenyl Sepharose 6 FF low sub 20 28951197 1 RTP Phenyl Sepharose 6 FF high sub 1 Phenyl Sepharose 6 FF high sub 28929108 2 5 RTP Phenyl Sepharose 6 FF high sub 2 5 28929109 10 RTP Phenyl Sepharose 6 FF high sub 10 28929110 20 RTP Phenyl Sepharose 6 FF high sub 20 28951208 1 RTP CM Sepharose FF 1 CM Sepharose FF 28929117 2 5 RTP CM Sepharose FF 2 5 28929118 10 RTP CM Sepharose FF 10 28...

Page 213: ... 10 RTP SP Sepharose FF 10 28929107 20 RTP SP Sepharose FF 20 RTP columns packed with Capto and Sepharose 6FF media can be requested by GE Healthcare even if not listed in ordering list above ÄKTA ready IQ OQ tools Product code Product 28933680 Low flow test kit ÄKTA ready 28933688 UV Cond test tools ÄKTA ready 28932942 Pressure calib tool ÄKTA ready 28938864 Temp test tool ÄKTA ready ÄKTA ready O...

Page 214: ... value and a desired setpoint to compute the adjustment required in order to keep a process within defined boundaries A typical PID controller is tuned using the following three parameters P The weight of the current error Proportional to the error I The weight of the stationary or long term change in the error Reduces effects of error trends D The weight of the current rate of change of the error...

Page 215: ...lowTune one for each flow kit size Default values for the PID parameters are D I P Column volume 0 25 0 3 1 l 0 25 0 3 2 5 l 0 15 0 3 10 l1 0 10 0 5 20 l1 1 ÄKTA ready High Flow Kits should be used for columns of 10 l or more It is usually not necessary to tune the PID parameters the default values can be used unchanged If tuning is required however use large adjustments for the I parameter To slo...

Page 216: ...ly accessible see Section 4 5 Positioning the system on page 66 2 Installation system unit Wheels of system unit locked see Section 4 5 Posi tioning the system on page 66 Connectedtocomputer see Computer on page 69 Compressed air connected see Compressed air on page 67 Mains power correctly con figured see Power on page67 andcableconnect ed to source 216 ÄKTA ready Operating Instructions 28960345 ...

Page 217: ... available Flow kit for testing avail able Performed installation test details below 5 Installation test Installation test checklist Calibration pressure sensors Perform pressure sensor calibration as described in Calibrating pressure sensors on page 188 Remark Sign Perform Pressure sensor Calibration at 0 bar g PT 111 Calibration at 5 bar g Calibration at 0 bar g PT 112 Calibration at 5 bar g Cal...

Page 218: ... 112 Calibration at 5 bar g Calibration at 0 bar g PT 113 Calibration at 5 bar g Temperature sensor check Verify that the temperature sensor is working and gives a realistic value This can be done by filling a plastic test tube with tempered water and holding it in front of the temperature sensor Then check that the temperature Temp_161 in UNICORN is re sponding Remark Sign Component TE 161 218 ÄK...

Page 219: ...the system cabinet Check that the flow diagram in UNICORN is updated and that the valve is in its correct state In the record table mark checked function with OK Not OK Remark Closed Open Valve XV 001 XV 002 XV 003 XV 004 XV 005 XV 006 XV 021 XV 022 XV 023 XV 031 XV 032 XV 033 XV 051 XV 052 XV 053 XV 054 XV 055 XV 056 HV 301 ÄKTA ready Operating Instructions 28960345 AD 219 14 Reference informatio...

Page 220: ...performance Perform a component test as described in Chapter 8 Component test on page 107 The test result printed report is attached to the ITR and filed in the customer log book Remark OK Not OK Component UV Conductivity Flow Pressure sen sors System serial number Installation preformed by Date Installation accepted 220 ÄKTA ready Operating Instructions 28960345 AD 14 Reference information 14 6 C...

Page 221: ... testorothersuitablesurvey Adequate space andclearance is providedto allowsafe access forinstrumentservice repairor installation Insome cases this mayrequirecustomerto move equipmentfrom normaloperatinglocation prior to GE arrival Consumables suchas columns orgels have beenremovedor isolatedfromthe instrument andfrom any areathat may impede access tothe instrument All buffer waste vessels are labe...

Page 222: ...us orhazardous biological substances pleasespecify OtherHazardous Chemicals please specify Equipment must be decontaminatedpriortoservice return Pleaseprovide atelephone number whereGE cancontact youfor additionalinformationconcerningthe system equipment Telephone No Liquid and orgas in equipment is Water Ethanol None empty Argon Helium Nitrogen Liquid Nitrogen Other please specify Equipment type ...

Page 223: ...not covered by this manual Please contact your local GE Healthcare representative for advice Contact information is found on the back cover of this manual Training GE Healthcare recommends that all new operators attend the training courses available from GE Healthcare The courses will provide all information necessary for inexperienced operators to safely operate ÄKTA ready Courses in the field of...

Page 224: ...ormity 9 CE marking 10 Center of gravity 63 Checklists 217 Chemical resistance 207 Chemicals 209 Column Adding to column list 139 Installation 122 127 Optional column rins ing 138 Optional column test 135 ReadyToProcess 125 Types 124 Components Drawing back 40 Drawing front 38 Component test 107 Run a test 108 Test report 113 Troubleshooting 117 Compressed air Connecting 59 Computer 69 Conductivit...

Page 225: ...on 100 Ordering information 210 Types 100 Gradient pump 46 98 Calibration 103 Layout 37 Gravity 63 I Installation 55 Finishing 71 Positioning 66 Installation test Checklist 220 Installing and moving precau tions 17 Instructions method edi tor 145 L Labels Safety labels 26 system label 24 System label 24 Layout 36 Layout gradient pump 37 Lifting 62 M Maintenance Daily 184 Spare parts 194 Yearly 187...

Page 226: ...ions 205 Service 223 Spare parts 210 223 Specifications 58 202 Starting the system 74 Storage 195 Stray light calibration 108 112 System Capacity 203 System label 24 System setup 82 T Temperature Changes 50 Recommended operating conditions 202 Sensor 50 Sensors checklist 218 Training 223 Troubleshooting 196 Typographical conventions 8 U UNICORN Alarms 54 Column efficiency test 168 Control system 5...

Page 227: ... reserved First published Sep 2009 All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare repre sentative for the most current information Any use of UNICORN is subject to GE Healthcare Standard Software End User License Agreemen...

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