8.1
Run a component test
General
The component test consists of a number of steps to assure correct function of the
components of the ÄKTA ready, in particular the sensors. The points being tested are:
•
Function of flow meter and size of flow kit.
•
Function of UV and conductivity sensors.
•
Function of pressure sensors.
A component test should always be performed after mounting a new flow kit.
Note:
The component test is included in the flow kit installation wizard.
What does the test do?
Description
Stage
Initially in the component test, a number of settings are made. Inlets are
opened and primed and pressures are set to zero.
1
Stray light calibration is performed. Stray light calibration shuts off the UV
LED lamp for approximately 10 seconds. During this time, stray light from
the outside is measured. The measured light is later subtracted from the UV
measurements made during the run.
Note:
Do not change anything on the system or in UNICORN during the time (ap-
proximately 10 seconds) stray light is being measured. Also note that if stray
light calibration is included in a user-defined method, a delay of at least 0.3
minutes is required before the next instruction.
2
After the air trap has been filled with the chosen liquid, the function of the
flow meter cell is tested — if the test fails, the test procedure will stop.
3
Next, the size of the flow meter cell is tested. If the selected component does
not match the actual component mounted on the system, the test procedure
will stop.
4
UV and conductivity test is performed by pumping a solution of 1% (v/v)
acetone and 1.0 M sodium chloride through the flow kit and the actual flow
cells. If the response is within specifications for both flow cells the test will
pass, otherwise the test will fail for one or both flow cells.
5
Finally, the component test checks that the three pressure sensors are
working and that they produce acceptable pressure readings.
6
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ÄKTA ready Operating Instructions 28960345 AD
8 Component test
8.1 Run a component test
