Fluidigm CyTOF XT, User Manual

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Contents
4
CyTOF XT User Guide
Cones 89
Periodic Maintenance 90
Autosampler Module Maintenance 91
Replace the Sample Loop 91
Replace the Probe Line 92
Recalibrate the Probe Line 93
Replace A Bottle Line 97
Clean the Carousel 98
Clean the Bottle Tray 101
Replace Syringes 102
Nebulizer Assembly Maintenance 108
Replace the Nebulizer Line 108
Replace the Nebulizer Gas Line 111
Clean the Nebulizer Mount 111
Remove and Clean the Torch Assembly 116
Remove and Disassemble Torch Assembly 116
Clean the Inner and Outer Torch 121
Reassemble the Torch Assembly 122
Fill the Chiller with Coolant 124
Check the Cooling System Air Filter 127
Check the Autosampler Air Filter 129
Appendix A: Troubleshooting 132
Appendix B: Recommendations for
Extended Shutdown
137
Expected Power Outages 137
CyTOF XT Shutdown Procedure 137
CyTOF XT Startup Procedure 138
Appendix C: Preventive Maintenance,
Decontamination, and Disposal
139
Preventive Maintenance Recommendations 139
Clean and Decontaminate the CyTOF XT System 139
Clean the System 139
Dispose of Products 140
Appendix D: Related Documents 141
Appendix E: Revision History 142
Appendix F: Safety 143
General Safety 143
Instrument Safety 143
Symbols Used in Documentations and on the
Instrument
144
Electrical Safety 145
Chemical Safety 146
Laboratory Ventilation Safety 146
Pressurized Gas Safety 147
Safe Handling of Gas Cylinders 147
Sample Handling and Preparation Safety 147
Radio Frequency Radiation Safety 147