Fluidigm CyTOF XT, User Manual

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CyTOF XT User Guide
3
Contents
About This User Guide 5
Safety Alert Conventions 5
Safety Alerts for Chemicals 5
Safety Alerts for Instruments 6
Safety Data Sheets 6
Chapter 1: Product Overview 7
CyTOF XT Site Requirement Specifications 8
CyTOF XT Specification 8
Instrument Specifications 8
Workstation Specifications 9
Data File Size Specifications 9
Instrument and Computer Connections 10
CyTOF XT Equipment and Consumables 11
Standard Equipment 11
Standard Consumables 12
Standard Reagents Consumables 12
CyTOF XT Special Tools and Kits 13
CyTOF XT Customer-Orderable Parts 13
Required Consumables from Other Suppliers 14
Consumables Ordering 14
Regulatory Compliance 15
General Regulations and Requirements 15
Harmonized Standards 15
Conformity Symbols on the Instrument 15
Chapter 2: Mass Cytometry and CyTOF XT
16
CyTOF XT Instrument Overview 16
CyTOF XT System Components Overview 17
Autosampler Module 17
Autosampler Module Functions 25
Nebulizer Assembly 28
Heater Assembly 30
Ionization 31
Vacuum Interface 34
Event Detection and Acquisition Data File Format 36
Data Acquisition and Analysis 37
Chapter 3: Annotated Figures of CyTOF XT
Components 38
Main Components of the CyTOF XT Instrument 38
CyTOF XT Status Panel 39
Autosampler Module 40
Nebulizer Assembly 44
Heater Assembly 45
Torch Assembly 46
Interior View of CyTOF XT 47
Other Components of CyTOF XT 48
Chapter 4: Maintenance 52
CyTOF XT Routine Cleaning 52
Clean the Nebulizer 53
Remove the Nebulizer 54
Materials Required for Cleaning 56
Daily Cleaning Method: 10% Contrad/Decon 56
Cleaning Method for Manual Clog Removal: Hot DIW
58
Clean the Nebulizer Rest 60
Clean the Injector and Spray Chamber 60
Remove the Heater Assembly 61
Remove the Injector Cap and Injector 62
Clean the Injector 63
Remove the Spray Chamber and Injector Holder 64
Clean the Spray Chamber and Injector Holder 66
Clean the Vacuum Interface Cones 66
Remove Cones 67
Clean Cones 70
Reinstall Cones 72
Reassemble the Injector and Spray Chamber 74
Replace the Nebulizer Port O-Ring 79
Check the Nebulizer Holder O-Ring 81
Reassemble the Nebulizer Assembly 83
Clean the Autosampler Fluidic Lines and Nebulizer
with 10% Bleach
85
Cleaning Summary 88
Maintenance Checklist 89
Nebulizer 89
Heater Assembly and Spray Chamber 89
Injector 89