Chapter 2: Mass Cytometry and CyTOF XT
CyTOF XT System Components Overview
18
CyTOF XT User Guide
Carousel
Figure 5. The Autosampler carousel
The carousel housing contains a removable carousel, which can hold 16 tubes.
•
Locations 1
–
12 are for polypropylene sample tubes (5 mL).
•
Location 13 is for a large-volume polypropylene sample tube (15 mL).
•
Location 14 is for EQ
™
Six Element Calibration Beads (EQ6) (15 mL).
•
Location 15 is for EQ Four Element Calibration Beads (EQ4) (15 mL).
•
Location 16 is reserved for the Autosampler wash tube.
NOTE
Locations 13
–
15 require 15 mL polypropylene screw cap tubes with a skirted bottom
[20 X 76 mm Conical Bottom Skirted Polypropylene Sterile, Sarstedt, Inc. (60.732.001)].
The carousel is insulated and chilled to 4
–
8 °C.
NOTE
For instructions on turning on or off the carousel chiller, refer to the CyTOF Software
Help content. To view the
Help
content, click the
icon in the upper right corner of the
CyTOF Software window.
NOTE
We recommend adding barcoded samples to the carousel in one of the following
ways:
•
As a suspension in a 15 mL tube at Location 13
•
As an aliquot of multiple samples in 5 mL tubes at Locations 1
–
12 in pelleted form with
concatenation of the acquired FCS files
The carousel lid has 2 parts: the main carousel lid and the corner lid. The main carousel lid is
hinged and can be opened to allow access to the carousel for inserting and removing tubes.
Summary of Contents for CyTOF XT
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