Chapter 2: Mass Cytometry and CyTOF XT
CyTOF XT System Components Overview
CyTOF XT User Guide
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Spray Chamber
The spray chamber is housed within the heater assembly. Aerosolized sample exits the
nebulizer directly into the spray chamber. Makeup gas (argon gas) is pumped into the spray
chamber, which heats the sample to 200 ˚C as it passes through the spray chamber
. This
high flow of heated gas partially evaporates the droplets to reduce their size as it directs the
aerosol to the plasma torch.
Figure 21. The spray chamber
Ionization
The aerosol cell droplets that exit the spray chamber are transmitted to the inductively
coupled plasma (ICP) source, where they are vaporized, atomized, and ionized in the plasma
for subsequent mass analysis. This results in the formation of an ion cloud containing the
ionized metal tags.
Summary of Contents for CyTOF XT
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