Chapter 2: Mass Cytometry and CyTOF XT
CyTOF XT System Components Overview
28
CyTOF XT User Guide
Nebulizer Assembly
For liquid sample analysis, it is critical to remove as much water as possible from cell
droplets so that they can be efficiently ionized in the plasma. This is achieved first by
aerosolizing the sample in the nebulizer followed by delivery to the plasma through the
spray chamber.
The CyTOF XT instrument employs a concentric glass nebulizer consisting of an inner
capillary that carries the liquid sample and an outer chamber that carries argon gas (called
nebulizer gas). The nebulizer gas flows at a rate of approximately 0.15
–
0.3 L/min. Both liquid
and gas flows are directed toward the heater assembly through the tapered end of the
nebulizer. The sample within the capillary travels at atmospheric pressure, and as the
sample exits the tip, shear force exerted by the exiting nebulizer gas onto the liquid breaks
it up into fine aerosol droplets.
IMPORTANT
Ensure that the nebulizer gas line is disconnected from the nebulizer
whenever the nebulizer is stored in the nebulizer rest to prevent DIW from backing up and
entering the gas port. Store the nebulizer soaked in DIW when not in use.
Figure 17. The concentric nebulizer. Liquid sample enters from the sample inlet (left), and argon
(nebulizer gas) enters from the side arm (bottom). The sample chamber narrows into a capillary, pulling
liquid rapidly to the tip (enlarged, at right, with liquid sample indicated in red), where shear forces
exerted by accelerated nebulizer gas break the liquid into aerosol droplets.
IMPORTANT
If the CyTOF XT system is used continuously, alternate nebulizers daily to
minimize clogging. After removing it from the system, soak the nebulizer in 10% Contrad® 100
(for 16 to 24 hr between uses), then thoroughly rinse with
Type 1 ultrapure (18.2 MΩ) water
(DIW) and soak the nebulizer in DIW when not in use.
Summary of Contents for CyTOF XT
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