25
Detach the silicone from
the short steel pipe here
and attach a 5ml syringe
with an 18-gauge needle.
17. Attach a 5ml syringe with an 18-gauge needle to the short silicone tubing on the P1 glass cannula and
use the syringe to drag buffer into the P1 glass cannula and silicone tube. When all air is removed,
re-connect the short silicone tube to the steel pipe inside the chamber again.
18. Close the P2 3-valve towards the chamber as shown below.
Connected to port of Pressure
Interface marked P2
19. Detach the silicone tube on the top of the chamber at the P2 side at the position shown in the figure
below with a red circle. Attach a 5ml syringe with an 18-gauge needle to the silicone tube and use the
syringe to drag buffer into the P2 glass cannula and silicone tube. When all air is removed, re-connect
the silicone tube to the steel pipe on the 114P chamber (red circle on the picture below).
