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FOR 112PP USERS CONTINUE AS FOLLOWS
12. Let the pressure regulator run and open the valve into the P1 Pulsation Chamber (see figure below).
As soon as the bottom part of P1 pulsation chamber is filled with buffer, quickly close the valve again.
NOTE: Do not fill the pulsation chamber completely, only the bottom area otherwise, the
pulsation will not work properly
13. Let the pressure regulator run until no air bubbles are visible in the perfusion circuit.
NOTE: Never fill the buffer bottle with more than 300 ml as higher volumes makes precise
control of the pressure more difficult
IMPORTANT:
NEVER RUN AIR BUBBLES THROUGH A VESSEL AS THIS MAY DAMAGE THE ENDOTHELIUM
14. When no air is seen in, the tubing turn of the flow and close the P1 3-way valve to the chamber as
shown below.
Connected to P1 Reservoir Bottle
15. Fill the 114P chamber with 6ml pre-heated buffer.
16. Detach the short silicone tubing from the short steel pipe connecting the P1 glass cannula to the steel
pipe inside the chamber.
