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Step 3 - This step initiates the heating process for the chamber.
In order for the calibration to be accurate, the transducer
must be heated to the same temperature as used in your
experiments. This allows for heat-induced expansion of the
electronic parts in the transducer. Otherwise, inaccurate
readings and transducer drift may occur. To start heating,
press HEAT ON.
Covering the chamber with the chamber cover will expedite
the heating. Place the temperature probe into the chamber
for the first calibration to monitor when the chamber has
reached the target temperature. After approximately 20-
30 minutes, the whole system will have reached the target
temperature (normally 37° C).
Press NEXT STEP when the chamber are heated and have
reached the target temperature.
Step 4 - This is the first step in the actual force calibration
process. A four- digit number will be displayed in blue at the
bottom of the screen. If nothing has been disturbed during
the heating process, the zero/0 gram calibration should be
stable. After 30 to 45 seconds, the four-digit number will not
fluctuate. If the four-digit number is not stable, please wait.
Press NEXT STEP
Step 5 - At this step, carefully place the 2 gram weight in
the pan closest to the transducer (over the transducer) to
simulate the stretch created by the contraction of a mounted
muscle preparation.
Remember, a 2 gram weight in a 90° vector is divided, and
the transducer will only detect 1 gram or 9.81 mN of force.
The weight placement should cause a positive increase in
the four-digit number. Wait at least 30 to 45 seconds for the
applied force to stabilize. Press NEXT STEP
